##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632211.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1892229
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.002364407267834	33.0	31.0	34.0	30.0	34.0
2	32.188126807061934	33.0	31.0	34.0	30.0	34.0
3	32.213414972500686	34.0	31.0	34.0	30.0	34.0
4	35.91667340475175	37.0	35.0	37.0	35.0	37.0
5	35.54787871869631	37.0	35.0	37.0	33.0	37.0
6	35.52849153035917	37.0	35.0	37.0	33.0	37.0
7	35.51844782000487	37.0	35.0	37.0	33.0	37.0
8	35.48117748961674	37.0	35.0	37.0	33.0	37.0
9	37.162037470094795	39.0	37.0	39.0	34.0	39.0
10	36.97931751389499	39.0	37.0	39.0	33.0	39.0
11	37.115057426981615	39.0	37.0	39.0	33.0	39.0
12	37.05929462026002	39.0	37.0	39.0	33.0	39.0
13	37.11766281988068	39.0	37.0	39.0	33.0	39.0
14	38.295747502020106	40.0	38.0	41.0	34.0	41.0
15	38.27289138893865	40.0	38.0	41.0	34.0	41.0
16	38.16944830673243	40.0	38.0	41.0	33.0	41.0
17	38.19208087393228	40.0	38.0	41.0	33.0	41.0
18	38.18996485097734	40.0	38.0	41.0	33.0	41.0
19	38.239578296284435	40.0	38.0	41.0	34.0	41.0
20	38.20276721263652	40.0	38.0	41.0	34.0	41.0
21	38.16006255056867	40.0	38.0	41.0	34.0	41.0
22	38.13573462831401	40.0	38.0	41.0	34.0	41.0
23	38.073788637633186	40.0	38.0	41.0	34.0	41.0
24	38.00581166444442	40.0	37.0	41.0	33.0	41.0
25	37.921956063457436	40.0	37.0	41.0	33.0	41.0
26	37.79235652767186	39.0	37.0	41.0	33.0	41.0
27	37.701766540941925	39.0	37.0	41.0	33.0	41.0
28	37.598446065460365	39.0	37.0	40.0	33.0	41.0
29	37.449234210024265	39.0	37.0	40.0	32.0	41.0
30	37.382636562487946	39.0	37.0	40.0	32.0	41.0
31	37.28448142376002	39.0	36.0	40.0	32.0	41.0
32	37.185927813176946	39.0	36.0	40.0	31.0	41.0
33	37.03537626788301	39.0	36.0	40.0	31.0	41.0
34	37.01319502026446	39.0	36.0	40.0	31.0	41.0
35	36.96324969123716	39.0	36.0	40.0	31.0	41.0
36	36.93162614038787	39.0	36.0	40.0	31.0	41.0
37	36.893005550596676	39.0	36.0	40.0	31.0	41.0
38	36.80725060233196	39.0	35.0	40.0	31.0	41.0
39	36.631745946183045	39.0	35.0	40.0	30.0	41.0
40	36.609876500148765	39.0	35.0	40.0	30.0	41.0
41	36.585183928583696	38.0	35.0	40.0	30.0	41.0
42	36.457084211266185	38.0	35.0	40.0	30.0	41.0
43	35.522861662092694	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	2.0
12	2.0
13	3.0
14	5.0
15	10.0
16	19.0
17	44.0
18	108.0
19	213.0
20	381.0
21	670.0
22	1254.0
23	2076.0
24	3255.0
25	4942.0
26	7264.0
27	10379.0
28	14754.0
29	20335.0
30	27523.0
31	36926.0
32	49544.0
33	67570.0
34	96164.0
35	140283.0
36	219211.0
37	355680.0
38	533480.0
39	300130.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.89364553656032	18.099236403204895	12.155399795690691	26.85171826454409
2	20.832943581352996	19.894209421798312	32.00643262522665	27.266414371622037
3	21.57101492472634	21.088092403192213	28.19066825421236	29.150224417869087
4	16.329735988614484	15.303168908202972	31.906444727355936	36.4606503758266
5	17.379027591269345	33.56554624202462	32.08417163038935	16.971254536316692
6	37.043825033862184	32.380647374075764	14.736429892999208	15.839097699062851
7	31.185231808623588	28.214185492347916	19.929353159686276	20.671229539342225
8	28.582322752690082	31.006765037424117	19.001505631717936	21.409406578167864
9	27.388334075843883	13.471783806294058	18.644572089319	40.49531002854306
10	18.499082299235454	25.02308124439484	31.526099642273742	24.951736814095966
11	38.50892254584408	20.05639909334441	19.745020290884455	21.689658069927052
12	23.474484325100185	23.809486061147993	27.12906313136518	25.586966482386647
13	32.45389432251593	18.032595420533138	22.477617666783463	27.03589259016747
14	24.354610356357504	19.42360042045651	22.868215210738235	33.35357401244775
15	27.37591486019927	25.736367004205096	20.227942812418583	26.65977532317706
16	27.290407239292918	23.974318119001452	22.172210657378148	26.563063984327478
17	25.896759853062186	24.36549698794385	23.354784225376527	26.382958933617445
18	26.095625846554515	23.238677771030886	24.298168984832174	26.36752739758243
19	26.567344650145408	23.649410298647787	24.045292615217292	25.737952435989513
20	26.905041620226726	23.124843768909578	23.777618882281164	26.192495728582532
21	27.447153595045844	23.296968812971368	23.20554224673652	26.050335345246268
22	27.475797062617684	23.23064491665649	23.089118706033997	26.204439314691825
23	26.655706048263717	23.052019602278584	23.46121954583721	26.83105480362049
24	26.513809903558183	23.714518697261273	23.154174256921333	26.61749714225921
25	26.77012137537264	23.02924223230909	23.390509288252108	26.810127104066154
26	26.643498223523686	23.567866257202486	23.42153090350058	26.36710461577325
27	26.64783173706777	23.203639728595217	23.4202097103469	26.728318823990122
28	26.312724305567663	23.288196090430915	23.88611526406159	26.512964339939828
29	25.948709167864987	23.58958667264903	24.137089115535172	26.32461504395081
30	25.935232997697426	23.789668163842748	23.821218256352694	26.453880582107136
31	26.75379142799312	23.529815894376423	23.309440876342133	26.40695180128832
32	25.884921962405183	23.343421964254855	23.5370560328586	27.23460004048136
33	25.622691545262228	23.13102695286881	24.214246795710245	27.032034706158715
34	26.39342278339461	22.706342625549023	24.13613785646452	26.764096734591845
35	26.16570193142585	22.91350571204648	24.62978846640655	26.29100389012112
36	25.69614988460699	22.976341658435633	24.386847469307362	26.940660987650016
37	25.682937953070162	22.141241889855827	25.15530625521541	27.0205139018586
38	25.11165403341773	21.99691474974752	26.043940770382445	26.847490446452305
39	25.057220875486003	21.752599711768504	26.373710581541665	26.81646883120383
40	24.757679963683042	21.753075341303827	26.990654936585372	26.49858975842776
41	24.123243011284575	21.77500714765496	27.475479976260804	26.626269864799664
42	24.015909279479388	22.079198659358884	27.51030662779188	26.394585433369848
43	23.318002207977997	21.50675208973121	27.8783910404079	27.29685466188289
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	52.0
1	59.0
2	66.0
3	164.0
4	262.0
5	262.0
6	349.5
7	437.0
8	441.0
9	445.0
10	683.5
11	922.0
12	922.0
13	1660.0
14	2398.0
15	3388.0
16	4378.0
17	4323.5
18	4269.0
19	4269.0
20	5281.5
21	6294.0
22	6245.0
23	6196.0
24	7685.5
25	9175.0
26	9175.0
27	11677.0
28	14179.0
29	17789.0
30	21399.0
31	26087.5
32	30776.0
33	30776.0
34	37736.0
35	44696.0
36	51979.0
37	59262.0
38	67394.0
39	75526.0
40	75526.0
41	83633.5
42	91741.0
43	98592.0
44	105443.0
45	113388.5
46	121334.0
47	121334.0
48	127901.0
49	134468.0
50	138682.5
51	142897.0
52	146189.0
53	149481.0
54	149481.0
55	149406.0
56	149331.0
57	147228.5
58	145126.0
59	141089.5
60	137053.0
61	137053.0
62	126131.0
63	115209.0
64	104500.5
65	93792.0
66	83511.5
67	73231.0
68	73231.0
69	63348.0
70	53465.0
71	45515.0
72	37565.0
73	30272.0
74	22979.0
75	22979.0
76	18783.0
77	14587.0
78	12148.0
79	9709.0
80	8145.5
81	6582.0
82	6582.0
83	5064.5
84	3547.0
85	2813.0
86	2079.0
87	1616.5
88	1154.0
89	1154.0
90	860.0
91	566.0
92	342.0
93	118.0
94	73.5
95	29.0
96	29.0
97	18.5
98	8.0
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1892229.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.40930344453143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.45991341253468	56.55282712188393
2	7.92214554524774	10.363640438017045
3	2.1767049493687147	4.27130263627415
4	1.026310000521612	2.685208890091013
5	0.5362127381617726	1.753665085062323
6	0.37964187415253814	1.4899266328016392
7	0.2568876850843443	1.1761991181391573
8	0.18159780169675943	0.9502548572834547
9	0.13285855752204395	0.7821167133745897
>10	0.810740034042046	9.925175539942197
>50	0.07024290093752919	3.1646086705854968
>100	0.04431468985676572	5.607894842192347
>500	0.002024842394471467	0.8743921890696308
>1k	4.049684788942931E-4	0.40278726528302944
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1983	0.10479704095011756	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1973	0.10426856368864444	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	5.2847726147310925E-5	0.0
13	0.0	0.0	0.0	5.2847726147310925E-5	0.0
14	5.2847726147310925E-5	0.0	0.0	1.0569545229462185E-4	0.0
15	5.2847726147310925E-5	0.0	0.0	1.5854317844193276E-4	0.0
16	5.2847726147310925E-5	0.0	0.0	6.87020439915042E-4	0.0
17	5.2847726147310925E-5	0.0	0.0	7.927158922096638E-4	0.0
18	5.2847726147310925E-5	5.2847726147310925E-5	0.0	8.455636183569748E-4	0.0
19	5.2847726147310925E-5	5.2847726147310925E-5	0.0	0.0011098022490935295	0.0
20	5.2847726147310925E-5	5.2847726147310925E-5	0.0	0.001374040879830084	0.0
21	5.2847726147310925E-5	5.2847726147310925E-5	0.0	0.0017439749628612605	0.0
22	5.2847726147310925E-5	5.2847726147310925E-5	0.0	0.002536690855070924	0.0
23	5.2847726147310925E-5	5.2847726147310925E-5	0.0	0.0038050362826063866	0.0
24	5.2847726147310925E-5	5.2847726147310925E-5	0.0	0.006077488506940756	0.0
25	5.2847726147310925E-5	5.2847726147310925E-5	0.0	0.008402788457422436	0.0
26	5.2847726147310925E-5	5.2847726147310925E-5	0.0	0.012207824740028824	0.0
27	5.2847726147310925E-5	5.2847726147310925E-5	0.0	0.032871285663627396	0.0
28	5.2847726147310925E-5	1.0569545229462185E-4	0.0	0.08846709357059848	0.0
29	5.2847726147310925E-5	1.5854317844193276E-4	0.0	0.1640393419612531	0.0
30	5.2847726147310925E-5	1.5854317844193276E-4	0.0	0.2703689669696427	0.0
31	5.2847726147310925E-5	1.5854317844193276E-4	0.0	0.5753003468396267	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1255	0.0	23.733068	1
GTATCAA	1775	0.0	16.780283	2
CTTATAC	1385	0.0	15.895307	37
ATACCGT	205	5.456968E-12	15.341464	6
TCTTATA	2340	0.0	15.021368	37
TTAGACT	125	2.9610092E-6	14.799999	4
TATATCG	75	0.004105711	14.799999	5
ATAAGAC	125	2.9610092E-6	14.799999	3
GTCTAAT	115	2.2112263E-5	14.478261	1
ATATCGC	90	8.2774984E-4	14.388889	6
GTAGCAC	225	2.7284841E-11	13.977777	3
TACCGTC	215	1.9826984E-10	13.767442	7
TGTAATA	150	1.3066601E-6	13.566666	2
GGACCGT	125	4.8476177E-5	13.32	6
CTCTTAT	3630	0.0	13.301653	37
TACTCGA	85	0.009407668	13.058823	2
TATACTG	100	0.0018332412	12.950001	5
GTACTAT	230	5.7661964E-10	12.869565	1
ACCGTCG	230	5.7661964E-10	12.869565	8
CGAATCG	105	0.0026446576	12.333333	16
>>END_MODULE