Basic Statistics
Measure | Value |
---|---|
Filename | ERR1632210.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 22185 |
Sequences flagged as poor quality | 0 |
Sequence length | 43 |
%GC | 51 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT | 521 | 2.3484336263240926 | Illumina PCR Primer Index 11 (95% over 22bp) |
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT | 388 | 1.7489294568402074 | No Hit |
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC | 279 | 1.2576064908722109 | No Hit |
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC | 277 | 1.24859139057922 | No Hit |
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATG | 211 | 0.951093080910525 | No Hit |
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT | 164 | 0.7392382240252422 | No Hit |
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT | 126 | 0.5679513184584178 | No Hit |
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTC | 108 | 0.486815415821501 | Illumina PCR Primer Index 11 (95% over 23bp) |
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT | 91 | 0.41018706333107957 | No Hit |
CACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCG | 84 | 0.3786342123056119 | No Hit |
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 56 | 0.2524228082037413 | No Hit |
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA | 36 | 0.16227180527383367 | No Hit |
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC | 31 | 0.1397340545413568 | No Hit |
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC | 27 | 0.12170385395537525 | No Hit |
TTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTA | 23 | 0.10367365336939373 | No Hit |
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT | 23 | 0.10367365336939373 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTCTTC | 30 | 7.949797E-6 | 37.0 | 37 |
TTATACA | 50 | 8.577363E-6 | 25.900002 | 2 |
TATACAC | 55 | 1.7958771E-5 | 23.545454 | 3 |
CCGTCTT | 70 | 4.739968E-6 | 21.142857 | 37 |
GCCGTCT | 70 | 4.739968E-6 | 21.142857 | 36 |
CAGTCGG | 45 | 0.0037103663 | 20.555555 | 10 |
GATTCCT | 45 | 0.0037103663 | 20.555555 | 18 |
ATTCCTC | 45 | 0.0037103663 | 20.555555 | 19 |
TCTTATA | 45 | 0.0037103663 | 20.555555 | 36 |
GGGCAGT | 50 | 0.0068246108 | 18.5 | 7 |
TGATTCC | 50 | 0.0068246108 | 18.5 | 17 |
GCAGTCG | 50 | 0.0068246108 | 18.5 | 9 |
CTTATTG | 50 | 0.0068246108 | 18.5 | 28 |
AGTCGGT | 50 | 0.0068246108 | 18.5 | 11 |
GTGATTC | 50 | 0.0068246108 | 18.5 | 16 |
GGCAGTC | 50 | 0.0068246108 | 18.5 | 8 |
GGTATCA | 240 | 0.0 | 18.5 | 1 |
CTTATAC | 75 | 1.954416E-4 | 17.266666 | 1 |
TACACAT | 90 | 4.1381492E-5 | 16.444445 | 2 |
ATACACA | 90 | 7.831746E-4 | 14.388888 | 1 |