FastQCFastQC Report
Fri 10 Feb 2017
ERR1632210.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632210.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22185
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT5212.3484336263240926Illumina PCR Primer Index 11 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT3881.7489294568402074No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC2791.2576064908722109No Hit
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC2771.24859139057922No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATG2110.951093080910525No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1640.7392382240252422No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1260.5679513184584178No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTC1080.486815415821501Illumina PCR Primer Index 11 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT910.41018706333107957No Hit
CACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCG840.3786342123056119No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.2524228082037413No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA360.16227180527383367No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC310.1397340545413568No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC270.12170385395537525No Hit
TTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTA230.10367365336939373No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT230.10367365336939373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC307.949797E-637.037
TTATACA508.577363E-625.9000022
TATACAC551.7958771E-523.5454543
CCGTCTT704.739968E-621.14285737
GCCGTCT704.739968E-621.14285736
CAGTCGG450.003710366320.55555510
GATTCCT450.003710366320.55555518
ATTCCTC450.003710366320.55555519
TCTTATA450.003710366320.55555536
GGGCAGT500.006824610818.57
TGATTCC500.006824610818.517
GCAGTCG500.006824610818.59
CTTATTG500.006824610818.528
AGTCGGT500.006824610818.511
GTGATTC500.006824610818.516
GGCAGTC500.006824610818.58
GGTATCA2400.018.51
CTTATAC751.954416E-417.2666661
TACACAT904.1381492E-516.4444452
ATACACA907.831746E-414.3888881