##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632210.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22185
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.66229434302456	34.0	31.0	34.0	31.0	34.0
2	32.80996168582376	34.0	31.0	34.0	31.0	34.0
3	32.75122830741492	34.0	31.0	34.0	31.0	34.0
4	36.26950642325896	37.0	37.0	37.0	35.0	37.0
5	36.19981969799414	37.0	35.0	37.0	35.0	37.0
6	36.25143114717151	37.0	36.0	37.0	35.0	37.0
7	36.22573811133649	37.0	36.0	37.0	35.0	37.0
8	36.23826910074374	37.0	36.0	37.0	35.0	37.0
9	37.99490646833446	39.0	38.0	39.0	35.0	39.0
10	37.960243407707914	39.0	38.0	39.0	35.0	39.0
11	38.02483660130719	39.0	38.0	39.0	35.0	39.0
12	37.97146720757269	39.0	38.0	39.0	35.0	39.0
13	38.054856885282845	39.0	38.0	39.0	35.0	39.0
14	39.27955826008564	40.0	39.0	41.0	36.0	41.0
15	39.308136128014425	40.0	39.0	41.0	36.0	41.0
16	39.12138832544512	40.0	39.0	41.0	36.0	41.0
17	39.19441063781834	40.0	39.0	41.0	36.0	41.0
18	39.32161370295245	40.0	39.0	41.0	37.0	41.0
19	39.389858012170386	40.0	39.0	41.0	37.0	41.0
20	39.42425061978815	40.0	39.0	41.0	37.0	41.0
21	39.45720081135902	40.0	39.0	41.0	37.0	41.0
22	39.35388776200135	40.0	39.0	41.0	36.0	41.0
23	39.346270002253775	40.0	39.0	41.0	36.0	41.0
24	39.284832093757046	40.0	39.0	41.0	36.0	41.0
25	39.173089925625426	40.0	39.0	41.0	36.0	41.0
26	39.09952670723462	40.0	39.0	41.0	36.0	41.0
27	38.977687626774845	40.0	38.0	41.0	35.0	41.0
28	38.915618661257604	40.0	38.0	41.0	35.0	41.0
29	38.75361730899256	40.0	38.0	41.0	35.0	41.0
30	38.67185034933514	40.0	38.0	41.0	35.0	41.0
31	38.572999774622495	40.0	38.0	41.0	35.0	41.0
32	38.44922244759973	40.0	38.0	41.0	35.0	41.0
33	38.264548118097814	40.0	38.0	41.0	34.0	41.0
34	38.235654721658776	40.0	38.0	41.0	34.0	41.0
35	38.23921568627451	40.0	38.0	41.0	34.0	41.0
36	38.18435880099166	40.0	38.0	41.0	34.0	41.0
37	38.0675231011945	40.0	37.0	41.0	34.0	41.0
38	37.976200135226506	40.0	37.0	41.0	34.0	41.0
39	37.75131845841785	40.0	37.0	41.0	33.0	41.0
40	37.6920892494929	40.0	37.0	41.0	33.0	41.0
41	37.62686499887311	40.0	37.0	41.0	33.0	41.0
42	37.394500788821276	40.0	36.0	41.0	33.0	41.0
43	36.438359251746675	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	2.0
21	7.0
22	5.0
23	9.0
24	17.0
25	32.0
26	36.0
27	62.0
28	92.0
29	137.0
30	170.0
31	224.0
32	300.0
33	396.0
34	639.0
35	896.0
36	1457.0
37	2964.0
38	5946.0
39	8791.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.83389677710165	16.614829839981972	17.696641875140863	24.854631507775522
2	19.2201938246563	20.5859815190444	33.739012846517916	26.454811809781383
3	18.318683795357224	21.92923146270002	37.02501690331305	22.727067838629704
4	12.990759522199685	18.381789497408157	30.453008789722784	38.174442190669374
5	13.657876943881	34.67207572684246	33.635339193148525	18.034708136128014
6	30.813612801442414	33.25670498084291	15.587108406581024	20.34257381113365
7	24.187514086094207	33.680414694613475	17.556907820599505	24.57516339869281
8	31.679062429569527	29.046653144016226	19.039891818796484	20.23439260761776
9	25.422582826233942	12.301104349785891	15.672751859364437	46.60356096461573
10	20.229885057471265	24.863646608068514	25.476673427991887	29.42979490646833
11	33.657876943881	21.099842235744873	17.372098264593195	27.870182555780932
12	20.766283524904214	31.39508677034032	22.844264142438583	24.99436556231688
13	35.235519495154385	16.736533693937343	22.79918864097363	25.22875816993464
14	25.066486364660808	22.059950416948386	22.289835474419654	30.58372774397115
15	30.21410863195853	24.192021636240703	19.436556231688076	26.15731350011269
16	26.716249718278117	22.835249042145595	23.051611449177372	27.39688979039892
17	20.238900157764256	32.756366914581925	21.410863195853054	25.593869731800766
18	24.119900833896775	20.84741942754113	24.76898805499211	30.26369168356998
19	24.23258958755916	27.040793328825785	26.481857110660357	22.2447599729547
20	26.33310795582601	22.416046878521524	25.729096236195627	25.521748929456837
21	32.25602884832094	19.008338967771017	22.81721884155961	25.918413342348433
22	29.87153482082488	25.40906017579446	21.78498985801217	22.93441514536849
23	25.895875591615958	21.42438584629254	22.943430245661485	29.736308316430023
24	25.99954924498535	24.737435203966644	26.558485463150777	22.70453008789723
25	24.701374802794682	24.91773720982646	22.54676583276989	27.83412215460897
26	25.83276988956502	21.343249943655625	24.421906693711968	28.402073473067386
27	25.711066035609647	26.16182105025918	22.55578093306288	25.57133198106829
28	22.22222222222222	21.487491548343478	27.031778228532794	29.25850800090151
29	24.759972954699123	22.005859815190444	28.226279017354067	25.00788821275637
30	23.777327022763128	23.281496506648637	28.740139734054544	24.201036736533695
31	23.331079558260086	22.528735632183906	24.782510705431598	29.35767410412441
32	26.39621365787694	23.55194951543836	27.703403200360604	22.348433626324095
33	21.320712192923146	22.307865675005633	30.047329276538203	26.324092855533017
34	23.493351363533918	24.64277665089024	27.180527383367142	24.6833446022087
35	22.772143340094658	22.59634888438134	31.020960108181207	23.6105476673428
36	23.58800991661032	24.187514086094207	23.772819472616632	28.451656524678835
37	20.79783637592968	25.188190218616178	27.351814288933962	26.662159116520172
38	22.280820374126662	21.721884155961234	28.67252648185711	27.32476898805499
39	23.89452332657201	22.03741266621591	27.4149199909849	26.65314401622718
40	20.608519269776878	20.076628352490424	33.46855983772819	25.846292540004505
41	21.23056118999324	21.969799414018482	28.929456840207347	27.870182555780932
42	20.77079107505071	20.13522650439486	33.639846743295024	25.45413567725941
43	19.33288257831868	20.86995717827361	32.63917061077304	27.15798963263466
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.5
2	2.0
3	7.0
4	12.0
5	12.0
6	17.0
7	22.0
8	22.0
9	22.0
10	38.0
11	54.0
12	54.0
13	97.0
14	140.0
15	221.5
16	303.0
17	258.5
18	214.0
19	214.0
20	229.5
21	245.0
22	168.0
23	91.0
24	103.0
25	115.0
26	115.0
27	132.5
28	150.0
29	160.0
30	170.0
31	204.5
32	239.0
33	239.0
34	244.0
35	249.0
36	300.0
37	351.0
38	464.5
39	578.0
40	578.0
41	696.0
42	814.0
43	1011.5
44	1209.0
45	1477.0
46	1745.0
47	1745.0
48	2077.0
49	2409.0
50	2282.5
51	2156.0
52	2271.5
53	2387.0
54	2387.0
55	1928.5
56	1470.0
57	1884.0
58	2298.0
59	1971.0
60	1644.0
61	1644.0
62	1228.5
63	813.0
64	731.5
65	650.0
66	590.0
67	530.0
68	530.0
69	460.5
70	391.0
71	352.0
72	313.0
73	245.5
74	178.0
75	178.0
76	143.5
77	109.0
78	90.5
79	72.0
80	47.0
81	22.0
82	22.0
83	15.5
84	9.0
85	7.0
86	5.0
87	3.0
88	1.0
89	1.0
90	1.5
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	22185.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.59544737435205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.80306967188349	67.73495605138608
2	5.855338691159587	8.735632183908045
3	1.4562813463049127	3.2589587559161597
4	0.5921807964227446	1.7669596574261888
5	0.38673031603118013	1.4424160468785217
6	0.223578463955526	1.0006761325219742
7	0.08459725663182066	0.4417399143565472
8	0.09063991781980785	0.5409060175794456
9	0.06042661187987189	0.4056795131845842
>10	0.38068765484319295	4.282172639170611
>50	0.01812798356396157	1.0412440838404327
>100	0.04229862831591033	7.000225377507324
>500	0.00604266118798719	2.3484336263240926
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT	521	2.3484336263240926	Illumina PCR Primer Index 11 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT	388	1.7489294568402074	No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC	279	1.2576064908722109	No Hit
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC	277	1.24859139057922	No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATG	211	0.951093080910525	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	164	0.7392382240252422	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	126	0.5679513184584178	No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTC	108	0.486815415821501	Illumina PCR Primer Index 11 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	91	0.41018706333107957	No Hit
CACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCG	84	0.3786342123056119	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56	0.2524228082037413	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	36	0.16227180527383367	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	31	0.1397340545413568	No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC	27	0.12170385395537525	No Hit
TTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTA	23	0.10367365336939373	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	23	0.10367365336939373	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.00450755014649538	0.0
4	0.0	0.0	0.0	0.00450755014649538	0.0
5	0.0	0.0	0.0	0.00450755014649538	0.0
6	0.0	0.0	0.0	0.00450755014649538	0.0
7	0.0	0.0	0.0	0.00450755014649538	0.0
8	0.0	0.0	0.0	0.00450755014649538	0.0
9	0.0	0.0	0.0	0.00450755014649538	0.0
10	0.0	0.0	0.0	0.00901510029299076	0.0
11	0.0	0.0	0.0	0.00901510029299076	0.0
12	0.0	0.0	0.0	0.00901510029299076	0.0
13	0.0	0.0	0.0	0.00901510029299076	0.0
14	0.0	0.0	0.0	0.00901510029299076	0.0
15	0.0	0.0	0.0	0.00901510029299076	0.0
16	0.0	0.0	0.0	0.013522650439486139	0.0
17	0.0	0.0	0.0	0.013522650439486139	0.0
18	0.0	0.0	0.0	0.01803020058598152	0.0
19	0.0	0.0	0.0	0.01803020058598152	0.0
20	0.0	0.0	0.0	0.01803020058598152	0.0
21	0.0	0.0	0.0	0.022537750732476897	0.0
22	0.0	0.0	0.0	0.04056795131845842	0.0
23	0.0	0.0	0.0	0.054090601757944556	0.0
24	0.0	0.0	0.0	0.08564345278341222	0.0
25	0.0	0.0	0.0	0.09465855307640297	0.0
26	0.0	0.0	0.0	0.1126887536623845	0.0
27	0.0	0.0	0.0	0.13071895424836602	0.0
28	0.0	0.0	0.0	0.19382465629930132	0.0
29	0.0	0.0	0.0	0.4011719630380888	0.0
30	0.0	0.0	0.0	0.8158665765156637	0.0
31	0.0	0.0	0.0	1.3838178949740816	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTC	30	7.949797E-6	37.0	37
TTATACA	50	8.577363E-6	25.900002	2
TATACAC	55	1.7958771E-5	23.545454	3
CCGTCTT	70	4.739968E-6	21.142857	37
GCCGTCT	70	4.739968E-6	21.142857	36
CAGTCGG	45	0.0037103663	20.555555	10
GATTCCT	45	0.0037103663	20.555555	18
ATTCCTC	45	0.0037103663	20.555555	19
TCTTATA	45	0.0037103663	20.555555	36
GGGCAGT	50	0.0068246108	18.5	7
TGATTCC	50	0.0068246108	18.5	17
GCAGTCG	50	0.0068246108	18.5	9
CTTATTG	50	0.0068246108	18.5	28
AGTCGGT	50	0.0068246108	18.5	11
GTGATTC	50	0.0068246108	18.5	16
GGCAGTC	50	0.0068246108	18.5	8
GGTATCA	240	0.0	18.5	1
CTTATAC	75	1.954416E-4	17.266666	1
TACACAT	90	4.1381492E-5	16.444445	2
ATACACA	90	7.831746E-4	14.388888	1
>>END_MODULE