FastQCFastQC Report
Fri 10 Feb 2017
ERR1632209.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632209.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2167128
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11350.024.4493391
TATACCG500.00703684318.55
TACGTTA1708.54925E-1116.3235319
CTTATAC13550.016.110737
GTATCAA17500.015.7514292
TCTATAC1659.749783E-1015.696973
ATAACGC2201.8189894E-1215.1363643
ATTACGT2006.184564E-1114.817
CGGTCGA750.004105964714.820
TAGAACA6350.014.2755914
TCTTATA21650.014.27020837
TAACGCT1956.730261E-1014.2307732
AACGCTA1956.730261E-1014.2307733
ACGCTAA1956.730261E-1014.2307734
AATTACG1956.730261E-1014.2307716
TCGTTTA1702.4398105E-814.14705930
TTAACGG1051.6566986E-414.09523835
ATCGTTT1854.9039954E-914.029
CGCTAAG2009.840733E-1013.87500135
CTCTTAT33500.013.86119437