##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632209.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2167128
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.487316392940336	34.0	31.0	34.0	31.0	34.0
2	32.652378631995894	34.0	31.0	34.0	31.0	34.0
3	32.66206333912902	34.0	31.0	34.0	31.0	34.0
4	36.207274328050765	37.0	37.0	37.0	35.0	37.0
5	36.05931167886715	37.0	35.0	37.0	35.0	37.0
6	36.08268547127812	37.0	35.0	37.0	35.0	37.0
7	36.07301645311214	37.0	35.0	37.0	35.0	37.0
8	36.04982123806254	37.0	35.0	37.0	35.0	37.0
9	37.779485106555775	39.0	38.0	39.0	35.0	39.0
10	37.68134138823364	39.0	37.0	39.0	35.0	39.0
11	37.79724363304798	39.0	37.0	39.0	35.0	39.0
12	37.757877245829505	39.0	37.0	39.0	35.0	39.0
13	37.78631534454818	39.0	37.0	39.0	35.0	39.0
14	39.122458387321835	40.0	38.0	41.0	36.0	41.0
15	39.13732506801629	40.0	39.0	41.0	36.0	41.0
16	39.01730308500467	40.0	38.0	41.0	36.0	41.0
17	39.06539023075702	40.0	38.0	41.0	36.0	41.0
18	39.07076047192413	40.0	38.0	41.0	36.0	41.0
19	39.121614413177255	40.0	39.0	41.0	36.0	41.0
20	39.09314262932323	40.0	39.0	41.0	36.0	41.0
21	39.06739149694896	40.0	39.0	41.0	36.0	41.0
22	39.04958959507699	40.0	39.0	41.0	36.0	41.0
23	39.005950271511416	40.0	39.0	41.0	36.0	41.0
24	38.95051838193222	40.0	38.0	41.0	35.0	41.0
25	38.883253319600875	40.0	38.0	41.0	35.0	41.0
26	38.78428777626426	40.0	38.0	41.0	35.0	41.0
27	38.70961059983536	40.0	38.0	41.0	35.0	41.0
28	38.64056253253153	40.0	38.0	41.0	35.0	41.0
29	38.50460701905933	40.0	38.0	41.0	34.0	41.0
30	38.45642758526492	40.0	38.0	41.0	34.0	41.0
31	38.38014090538261	40.0	38.0	41.0	34.0	41.0
32	38.304949684559475	40.0	38.0	41.0	34.0	41.0
33	38.17110710581009	40.0	38.0	41.0	34.0	41.0
34	38.16506823777829	40.0	38.0	41.0	34.0	41.0
35	38.136088869692976	40.0	38.0	41.0	34.0	41.0
36	38.1120635237051	40.0	38.0	41.0	34.0	41.0
37	38.07596874757744	40.0	38.0	41.0	33.0	41.0
38	38.00069262175561	40.0	37.0	41.0	33.0	41.0
39	37.81046758659387	40.0	37.0	41.0	33.0	41.0
40	37.82107517414754	40.0	37.0	41.0	33.0	41.0
41	37.81602517248635	40.0	37.0	41.0	33.0	41.0
42	37.68793075443629	40.0	37.0	41.0	33.0	41.0
43	36.813530626709635	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	1.0
13	1.0
14	1.0
15	3.0
16	13.0
17	36.0
18	62.0
19	115.0
20	211.0
21	424.0
22	777.0
23	1259.0
24	2048.0
25	3202.0
26	4745.0
27	6905.0
28	9910.0
29	13579.0
30	19046.0
31	25252.0
32	34015.0
33	46537.0
34	66768.0
35	97883.0
36	155268.0
37	280412.0
38	634646.0
39	764004.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.025884950035255	17.982140418101746	12.145198622324108	26.846776009538893
2	19.98991291700352	20.19059326444954	32.817350890210456	27.002142928336493
3	20.92276044608348	21.18628895016815	29.150423971265194	28.740526632483178
4	15.950742180434197	15.26162737041836	32.71772594881336	36.06990450033408
5	16.561642874809426	34.35385450236442	32.83271684921241	16.25178577361374
6	36.23754572872484	33.019830854476524	15.065653713117083	15.676969703681554
7	30.58144235135165	28.949282183608904	20.12017748836248	20.349097976676966
8	27.98311867134752	31.772096525908943	19.547484043397528	20.69730075934601
9	27.043257251071463	13.795724110435561	18.869812950596366	40.29120568789661
10	17.8843150935247	25.866077130654023	32.200589905164804	24.049017870656463
11	37.11183649512165	20.52513741689462	20.440601570373325	21.922424517610402
12	22.791085713441937	24.21379816974355	27.62914788605011	25.365968230764402
13	31.724014455998905	18.52622457003001	23.28676478731298	26.4629961866581
14	24.008595708236893	19.83574574275262	23.304391803345258	32.85126674566523
15	26.911839079186834	26.184378587697637	20.865218851862927	26.03856348125261
16	26.669998264984805	24.850401083830768	22.485104710012514	25.99449594117191
17	25.314148495151183	25.074937890147698	23.983862512966468	25.627051101734644
18	25.171009741925722	23.9489314890491	24.962069614715883	25.917989154309296
19	26.074832681779757	24.207430294841835	24.77915471536522	24.938582308013185
20	26.325025563787648	23.745390212299412	24.549911218903546	25.379673005009394
21	26.39401087522287	24.211398680650152	23.66722224067983	25.727368203447142
22	26.719003215315386	23.80616188799185	23.5999442580226	25.874890638670166
23	26.04040924209368	24.032267591023697	23.811606882473026	26.115716284409597
24	26.365955310438515	24.029268229656946	23.746543812825085	25.858232647079454
25	26.422574024238532	23.592653502700347	23.934672986551785	26.050099486509332
26	26.052360543539656	24.051832655939105	23.863934202317537	26.031872598203705
27	26.07838577139883	23.805146719529258	23.846768626495525	26.269698882576385
28	25.680439734062777	23.92641320678797	24.30659379602866	26.086553263120592
29	25.49014179134781	24.21601308275284	24.46625210878176	25.827593017117582
30	25.49018793536884	24.790690720621946	24.095161891683368	25.623959452325845
31	26.097535540124994	24.147443067506856	24.00001292032589	25.755008472042263
32	25.323423443377596	24.016624767895575	24.390437482234553	26.26951430649228
33	25.421479488059774	23.81372950744026	24.54308190379156	26.221709100708402
34	25.939446124086814	23.484030477203007	24.43090578867515	26.145617610035032
35	25.662074413694068	23.61138797523727	24.871719621545196	25.85481798952346
36	25.288769283586387	23.713227829643657	24.822806959256674	26.17519592751328
37	25.67951685364224	22.578361776507894	25.239533613150677	26.502587756699185
38	24.776709082250793	23.067349967330035	26.10016574932353	26.055775201095642
39	24.804672358993102	22.74360351580525	26.42294317640675	26.028780948794903
40	24.36062844465117	22.35280980172837	27.246429375652937	26.040132377967524
41	23.77423022544123	22.46161740330982	27.677875972254522	26.08627639899443
42	24.074812378410503	22.276533734970894	27.380939196946375	26.26771468967223
43	23.25128003514329	22.13311811761926	27.896506343880013	26.719095503357437
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	30.0
1	47.0
2	64.0
3	172.5
4	281.0
5	281.0
6	358.0
7	435.0
8	478.5
9	522.0
10	749.0
11	976.0
12	976.0
13	1640.0
14	2304.0
15	3514.5
16	4725.0
17	5053.5
18	5382.0
19	5382.0
20	7057.5
21	8733.0
22	9983.5
23	11234.0
24	14123.0
25	17012.0
26	17012.0
27	20041.0
28	23070.0
29	29076.0
30	35082.0
31	40931.0
32	46780.0
33	46780.0
34	54092.5
35	61405.0
36	68896.5
37	76388.0
38	85785.5
39	95183.0
40	95183.0
41	104132.5
42	113082.0
43	116275.0
44	119468.0
45	127738.0
46	136008.0
47	136008.0
48	143168.0
49	150328.0
50	158818.0
51	167308.0
52	175757.0
53	184206.0
54	184206.0
55	174223.0
56	164240.0
57	158342.0
58	152444.0
59	143127.0
60	133810.0
61	133810.0
62	126120.5
63	118431.0
64	107505.5
65	96580.0
66	85444.5
67	74309.0
68	74309.0
69	64849.0
70	55389.0
71	48399.5
72	41410.0
73	34289.0
74	27168.0
75	27168.0
76	21889.0
77	16610.0
78	14099.5
79	11589.0
80	9303.5
81	7018.0
82	7018.0
83	5558.0
84	4098.0
85	3138.0
86	2178.0
87	1627.5
88	1077.0
89	1077.0
90	815.0
91	553.0
92	338.0
93	123.0
94	94.0
95	65.0
96	65.0
97	43.0
98	21.0
99	15.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2167128.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.4204658414647
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.90671410527429	52.15009933414009
2	9.088863878169153	11.164845067336216
3	2.4514969766453882	4.517162589435062
4	1.0610986009827512	2.606926815043482
5	0.59540009742716	1.8284875673014824
6	0.3752129409109242	1.3827452172296961
7	0.2525400693309471	1.0857790091359794
8	0.17010404795517312	0.8358295893540467
9	0.13661835720940546	0.755204682806758
>10	0.7954872500677236	9.301469077666297
>50	0.08723247745954349	3.764855870027156
>100	0.07509091075307178	8.708650584245127
>500	0.0036133420926431595	1.434890316473272
>1k	5.269457218437848E-4	0.46305427980536246
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	4.61440210269075E-5	0.0
11	0.0	0.0	0.0	4.61440210269075E-5	0.0
12	0.0	0.0	0.0	1.384320630807225E-4	0.0
13	0.0	0.0	0.0	1.384320630807225E-4	0.0
14	0.0	0.0	0.0	1.384320630807225E-4	0.0
15	0.0	0.0	0.0	1.8457608410763E-4	0.0
16	0.0	0.0	0.0	4.61440210269075E-4	0.0
17	0.0	0.0	0.0	5.5372825232289E-4	0.0
18	0.0	0.0	0.0	6.46016294376705E-4	0.0
19	0.0	0.0	0.0	8.30592378484335E-4	0.0
20	0.0	0.0	0.0	8.767363995112425E-4	0.0
21	0.0	0.0	0.0	0.00129203258875341	4.61440210269075E-5
22	0.0	0.0	0.0	0.0019841929041570228	4.61440210269075E-5
23	0.0	0.0	0.0	0.0027686412616144502	4.61440210269075E-5
24	0.0	0.0	0.0	0.0041068178713947676	4.61440210269075E-5
25	0.0	0.0	0.0	0.005444994481175085	4.61440210269075E-5
26	0.0	0.0	0.0	0.00904422812127387	9.2288042053815E-5
27	0.0	0.0	0.0	0.02750183653203687	1.384320630807225E-4
28	0.0	0.0	0.0	0.08158262917557246	1.384320630807225E-4
29	0.0	0.0	0.0	0.15093709277901443	1.384320630807225E-4
30	0.0	0.0	0.0	0.24410187123234067	1.384320630807225E-4
31	0.0	0.0	0.0	0.5114603290622427	1.384320630807225E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1135	0.0	24.449339	1
TATACCG	50	0.007036843	18.5	5
TACGTTA	170	8.54925E-11	16.32353	19
CTTATAC	1355	0.0	16.1107	37
GTATCAA	1750	0.0	15.751429	2
TCTATAC	165	9.749783E-10	15.69697	3
ATAACGC	220	1.8189894E-12	15.136364	3
ATTACGT	200	6.184564E-11	14.8	17
CGGTCGA	75	0.0041059647	14.8	20
TAGAACA	635	0.0	14.275591	4
TCTTATA	2165	0.0	14.270208	37
TAACGCT	195	6.730261E-10	14.23077	32
AACGCTA	195	6.730261E-10	14.23077	33
ACGCTAA	195	6.730261E-10	14.23077	34
AATTACG	195	6.730261E-10	14.23077	16
TCGTTTA	170	2.4398105E-8	14.147059	30
TTAACGG	105	1.6566986E-4	14.095238	35
ATCGTTT	185	4.9039954E-9	14.0	29
CGCTAAG	200	9.840733E-10	13.875001	35
CTCTTAT	3350	0.0	13.861194	37
>>END_MODULE