FastQCFastQC Report
Fri 10 Feb 2017
ERR1632208.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632208.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26497
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5632.1247688417556705No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4811.8152998452655018No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3271.2341019738083556No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2260.8529267464241234No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.47929954334452957No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT1050.39627127599350875RNA PCR Primer, Index 27 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT920.34720911801336No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.2113446805298713No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.20379665622523307No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC470.17737857115899913RNA PCR Primer, Index 27 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC430.1622825225497226No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.13963844963580782No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.13209042533116958No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA310.11699437672189304No Hit
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGC300.1132203645695739No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.1132203645695739No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.10944635241725478No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG280.10567234026493565No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA280.10567234026493565No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT270.10189832811261654No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC270.10189832811261654RNA PCR Primer, Index 27 (95% over 24bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCCC500.00685915718.4999985
TCTCCGA500.00685915718.4999981
TCCGAGC500.00685915718.4999983
CTCCGAG500.00685915718.4999982
GGTATCA3050.017.5901641
TCTTATA700.002503849815.85714237
GTATCAA3750.014.3066662
AACGCAG4150.012.4819287
TATCAAC4500.011.9222223
TCTCTTA1401.3019193E-411.89285737
ATCAACG4600.011.6630444
CAACGCA4600.011.6630446
CAGAGTA4450.011.640450511
AGAGTAC4450.011.640450512
CGCAGAG4450.011.64045059
GCAGAGT4500.011.51111110
TCAACGC4800.011.1770835
AGTACAT2553.0089723E-810.88235314
GAGTACA2553.0089723E-810.88235313
ACGCAGA4850.010.6804128
Produced by FastQC (version 0.11.5)