##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632208.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26497
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70898592293467	34.0	31.0	34.0	31.0	34.0
2	32.81714911122014	34.0	31.0	34.0	31.0	34.0
3	32.83005623278107	34.0	31.0	34.0	31.0	34.0
4	36.27825791599049	37.0	37.0	37.0	35.0	37.0
5	36.184964335585164	37.0	35.0	37.0	35.0	37.0
6	36.27576706796996	37.0	37.0	37.0	35.0	37.0
7	36.21919462580669	37.0	36.0	37.0	35.0	37.0
8	36.2660678567385	37.0	37.0	37.0	35.0	37.0
9	38.049930180775185	39.0	38.0	39.0	35.0	39.0
10	37.997848813073176	39.0	38.0	39.0	35.0	39.0
11	38.068083179227834	39.0	38.0	39.0	37.0	39.0
12	38.03883458504736	39.0	38.0	39.0	35.0	39.0
13	38.091746235422875	39.0	38.0	39.0	37.0	39.0
14	39.35302109672793	40.0	39.0	41.0	37.0	41.0
15	39.37996754349549	40.0	39.0	41.0	37.0	41.0
16	39.31648865909348	40.0	39.0	41.0	36.0	41.0
17	39.36090878212628	40.0	39.0	41.0	37.0	41.0
18	39.41359399177265	40.0	39.0	41.0	37.0	41.0
19	39.45065479110843	40.0	39.0	41.0	37.0	41.0
20	39.473902705966715	41.0	39.0	41.0	37.0	41.0
21	39.43770992942597	41.0	39.0	41.0	37.0	41.0
22	39.364531833792505	41.0	39.0	41.0	36.0	41.0
23	39.29716571687361	40.0	39.0	41.0	36.0	41.0
24	39.2154583537759	40.0	39.0	41.0	35.0	41.0
25	39.14077065328151	40.0	39.0	41.0	35.0	41.0
26	38.94331433747217	40.0	38.0	41.0	35.0	41.0
27	38.781862097595955	40.0	38.0	41.0	35.0	41.0
28	38.65981054458995	40.0	38.0	41.0	35.0	41.0
29	38.50828395667434	40.0	38.0	41.0	35.0	41.0
30	38.312488206212024	40.0	37.0	41.0	35.0	41.0
31	38.03834396346756	40.0	37.0	41.0	34.0	41.0
32	37.81394120089067	40.0	36.0	41.0	34.0	41.0
33	37.581990414009134	40.0	35.0	41.0	33.0	41.0
34	37.35913499641469	40.0	35.0	41.0	33.0	41.0
35	37.237158923651734	40.0	35.0	41.0	33.0	41.0
36	37.055213797788426	40.0	35.0	41.0	33.0	41.0
37	36.843906857380084	39.0	35.0	41.0	33.0	41.0
38	36.67830320413632	39.0	35.0	41.0	32.0	41.0
39	36.40555534588822	39.0	35.0	41.0	31.0	41.0
40	36.14265765935766	39.0	35.0	41.0	30.0	41.0
41	35.81375250028305	39.0	35.0	41.0	28.0	41.0
42	35.50647243084123	39.0	35.0	41.0	25.0	41.0
43	34.55640261161641	38.0	35.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	5.0
21	6.0
22	7.0
23	16.0
24	39.0
25	40.0
26	71.0
27	145.0
28	186.0
29	271.0
30	367.0
31	397.0
32	492.0
33	644.0
34	943.0
35	1240.0
36	1886.0
37	4113.0
38	6036.0
39	9592.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.93338868551157	18.719100275502885	14.88847794089897	21.459033098086575
2	20.240027172887494	21.91946258066951	32.9848662112692	24.855644035173793
3	21.953428690040383	23.65928218288863	31.78473034683172	22.602558780239274
4	14.594104993018078	18.232252707853718	30.82613126014266	36.347511038985544
5	13.903460769143678	36.97399705627052	31.550741593387933	17.57180058119787
6	30.263803449447106	32.271577914480886	17.549156508283957	19.915462127788054
7	25.429293882326302	33.55096803411707	20.255123221496774	20.764614862059855
8	30.42231195984451	31.04502396497717	19.590897082688606	18.94176699248972
9	27.018152998452656	12.782579159904895	20.8325470808016	39.36672076084085
10	20.53440012076839	27.03702305921425	28.633430199645243	23.79514662037212
11	32.0375891610371	23.006378080537417	19.039891308450013	25.91614144997547
12	20.198513039211985	30.9921877948447	26.52375740649885	22.285541759444467
13	32.96599615050761	18.998377174774504	26.621881722459147	21.413744952258746
14	23.01015209268974	22.961089934709587	27.614446918519075	26.414311054081598
15	28.769294637128734	24.923576253915538	24.587689172359134	21.719439936596597
16	22.576140695173038	24.591463184511454	29.074989621466578	23.757406498848926
17	20.466467902026643	29.63731743216213	27.62576895497604	22.270445710835187
18	20.700456655470433	22.806355436464507	31.309204815639507	25.18398309242556
19	22.3119598445107	26.459599199909423	32.49801864362003	18.730422311959842
20	22.908253764577122	22.5685926708684	33.501905876136924	21.021247688417557
21	25.78782503679662	22.859191606596973	30.63365664037438	20.719326716232025
22	24.100841604709967	23.74985847454429	30.69026682265917	21.459033098086575
23	22.379892063252445	24.542401026531305	31.460165301732275	21.61754160848398
24	21.74585802166283	25.572706344114426	31.192210438917613	21.489225195305128
25	21.198626259576557	25.704796769445597	31.10540815941427	21.991168811563576
26	22.327055893119976	24.323508321696796	31.603577763520395	21.74585802166283
27	21.91568856851719	24.980186436200324	31.060120013586445	22.04400498169604
28	21.111823980073215	25.119824885836135	32.67162320262671	21.096727931463942
29	21.534513341132957	24.919802241763218	32.68671925123599	20.858965165867836
30	20.587236290900858	25.863305279843	33.0641204664679	20.485337962788243
31	20.179642978450392	26.587915613088274	31.9583349058384	21.27410650262294
32	20.485337962788243	26.74265011133336	32.10552137977884	20.66649054609956
33	20.04755255311922	25.90859342567083	33.2226289768653	20.821225044344644
34	20.13812884477488	26.30486470166434	31.414877155904442	22.14212929765634
35	19.609767143450203	26.535079442955805	31.875306638487377	21.979846775106616
36	19.63241121636412	26.897384609578445	30.69026682265917	22.77993735139827
37	18.862512737291016	26.935124731101634	31.143148280937467	23.05921425066989
38	19.74940559308601	25.289655432690495	32.486696607163076	22.47424236706042
39	19.62486319205948	24.844321998716836	33.34339736573952	22.18741744348417
40	19.024795259840737	24.70468354908103	33.5585160584217	22.712005132656525
41	17.80578933464166	25.542514246895877	33.283013171302414	23.368683247160053
42	17.937879759972827	24.938672302524814	33.962335358719855	23.161112578782504
43	17.496320338151488	26.07464996037287	33.07166849077254	23.357361210703097
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	4.0
2	7.0
3	35.5
4	64.0
5	64.0
6	95.5
7	127.0
8	121.5
9	116.0
10	183.0
11	250.0
12	250.0
13	469.0
14	688.0
15	898.0
16	1108.0
17	957.5
18	807.0
19	807.0
20	883.5
21	960.0
22	672.5
23	385.0
24	353.5
25	322.0
26	322.0
27	341.0
28	360.0
29	369.5
30	379.0
31	421.0
32	463.0
33	463.0
34	482.0
35	501.0
36	575.0
37	649.0
38	744.5
39	840.0
40	840.0
41	967.5
42	1095.0
43	1291.5
44	1488.0
45	1603.5
46	1719.0
47	1719.0
48	2018.5
49	2318.0
50	2182.0
51	2046.0
52	2045.5
53	2045.0
54	2045.0
55	1785.0
56	1525.0
57	1562.0
58	1599.0
59	1391.5
60	1184.0
61	1184.0
62	1042.0
63	900.0
64	795.5
65	691.0
66	623.0
67	555.0
68	555.0
69	505.5
70	456.0
71	403.0
72	350.0
73	282.5
74	215.0
75	215.0
76	177.5
77	140.0
78	112.0
79	84.0
80	59.5
81	35.0
82	35.0
83	25.5
84	16.0
85	11.0
86	6.0
87	4.0
88	2.0
89	2.0
90	1.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	26497.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.23504547684644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.53465346534654	69.78148469638073
2	5.01980198019802	7.653696644903196
3	1.3613861386138615	3.1135600256632827
4	0.6089108910891089	1.856813978941012
5	0.4158415841584159	1.585085103974035
6	0.2871287128712871	1.3133562290070575
7	0.15841584158415842	0.8453787221194852
8	0.12376237623762376	0.7548024304638261
9	0.07920792079207921	0.5434577499339548
>10	0.36633663366336633	4.887345737253274
>50	0.01485148514851485	0.7623504547684644
>100	0.024752475247524754	4.77789938483602
>500	0.0049504950495049506	2.1247688417556705
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	563	2.1247688417556705	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	481	1.8152998452655018	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	327	1.2341019738083556	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	226	0.8529267464241234	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	127	0.47929954334452957	No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT	105	0.39627127599350875	RNA PCR Primer, Index 27 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT	92	0.34720911801336	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56	0.2113446805298713	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54	0.20379665622523307	No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC	47	0.17737857115899913	RNA PCR Primer, Index 27 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC	43	0.1622825225497226	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37	0.13963844963580782	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35	0.13209042533116958	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	31	0.11699437672189304	No Hit
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGC	30	0.1132203645695739	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30	0.1132203645695739	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29	0.10944635241725478	No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG	28	0.10567234026493565	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	28	0.10567234026493565	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	27	0.10189832811261654	No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC	27	0.10189832811261654	RNA PCR Primer, Index 27 (95% over 24bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.007548024304638261	0.0
23	0.0	0.0	0.0	0.015096048609276522	0.0
24	0.0	0.0	0.0	0.022644072913914782	0.0
25	0.0	0.0	0.0	0.030192097218553045	0.0
26	0.0	0.0	0.0	0.045288145827829564	0.0
27	0.0	0.0	0.0	0.08680227950334	0.0
28	0.0	0.0	0.0	0.17360455900668	0.0
29	0.0	0.0	0.0	0.317017020794807	0.0
30	0.0	0.0	0.0	0.5811978714571461	0.0
31	0.0	0.0	0.0	1.1208816092387817	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGCCC	50	0.006859157	18.499998	5
TCTCCGA	50	0.006859157	18.499998	1
TCCGAGC	50	0.006859157	18.499998	3
CTCCGAG	50	0.006859157	18.499998	2
GGTATCA	305	0.0	17.590164	1
TCTTATA	70	0.0025038498	15.857142	37
GTATCAA	375	0.0	14.306666	2
AACGCAG	415	0.0	12.481928	7
TATCAAC	450	0.0	11.922222	3
TCTCTTA	140	1.3019193E-4	11.892857	37
ATCAACG	460	0.0	11.663044	4
CAACGCA	460	0.0	11.663044	6
CAGAGTA	445	0.0	11.6404505	11
AGAGTAC	445	0.0	11.6404505	12
CGCAGAG	445	0.0	11.6404505	9
GCAGAGT	450	0.0	11.511111	10
TCAACGC	480	0.0	11.177083	5
AGTACAT	255	3.0089723E-8	10.882353	14
GAGTACA	255	3.0089723E-8	10.882353	13
ACGCAGA	485	0.0	10.680412	8
>>END_MODULE