FastQCFastQC Report
Fri 10 Feb 2017
ERR1632207.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632207.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences837011
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC21740.259733743045193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10880.12998634426548755No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT10420.12449059809249818No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG9690.11576908786144985No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9570.11433541494675697No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT9320.11134859637448014No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG9180.10967597797400513No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG8950.10692810488751044No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC8860.10585285020149077No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT8780.1048970682583622No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT8550.10214919517186752No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT8450.10095446774295677No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTA250.005495500329.68
CCGTTAT250.005495500329.69
TCGTATG451.3227922E-424.66666830
GGTATCA7200.022.6111131
CTCGTAT502.7012517E-422.229
CTACTAG450.003824951820.5555571
TCTCGTA555.14149E-420.18181828
GTATCAA8750.018.6057152
CTAAACG609.2339556E-418.526
CAGGACA7250.017.3517254
TGCATCA6550.017.22900814
ACAGGCT7350.017.1156468
TATGCCG650.001579449317.07692333
TCGGAAC1201.0401709E-716.95833432
GTTAACG803.381191E-416.187513
GCTGCAT7050.016.00709212
CTGCATC7050.016.00709213
CCAGGAC8900.016.005623
GTTTTCG1059.336947E-615.85714428
CGTATGC700.00259170415.85714331
Produced by FastQC (version 0.11.5)