##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632207.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	837011
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.833500396052145	34.0	31.0	34.0	31.0	34.0
2	33.004522043318424	34.0	33.0	34.0	31.0	34.0
3	33.07562744097748	34.0	33.0	34.0	31.0	34.0
4	36.44652818182796	37.0	37.0	37.0	35.0	37.0
5	36.32074488865738	37.0	37.0	37.0	35.0	37.0
6	36.35386153825935	37.0	37.0	37.0	35.0	37.0
7	36.332748315135646	37.0	37.0	37.0	35.0	37.0
8	36.31750956677989	37.0	37.0	37.0	35.0	37.0
9	38.1202839628153	39.0	39.0	39.0	37.0	39.0
10	38.101363064523646	39.0	38.0	39.0	37.0	39.0
11	38.16270156545135	39.0	39.0	39.0	37.0	39.0
12	38.13708541464808	39.0	38.0	39.0	37.0	39.0
13	38.149917981962005	39.0	39.0	39.0	37.0	39.0
14	39.5811536526999	41.0	39.0	41.0	37.0	41.0
15	39.58753110771543	41.0	39.0	41.0	37.0	41.0
16	39.58383223159552	41.0	39.0	41.0	37.0	41.0
17	39.56481694983698	41.0	39.0	41.0	37.0	41.0
18	39.52759760624412	41.0	39.0	41.0	37.0	41.0
19	39.55119825187482	41.0	39.0	41.0	37.0	41.0
20	39.5171951145206	41.0	39.0	41.0	37.0	41.0
21	39.504706628706195	41.0	39.0	41.0	37.0	41.0
22	39.462513634826784	41.0	39.0	41.0	37.0	41.0
23	39.41844730833884	40.0	39.0	41.0	37.0	41.0
24	39.37395087997649	41.0	39.0	41.0	36.0	41.0
25	39.31265658396365	40.0	39.0	41.0	36.0	41.0
26	39.22359921195779	40.0	39.0	41.0	36.0	41.0
27	39.15657619792332	40.0	39.0	41.0	36.0	41.0
28	39.086267683459354	40.0	39.0	41.0	35.0	41.0
29	39.03102946078367	40.0	39.0	41.0	35.0	41.0
30	38.96968259676396	40.0	39.0	41.0	35.0	41.0
31	38.913795637094374	40.0	38.0	41.0	35.0	41.0
32	38.830243569080935	40.0	38.0	41.0	35.0	41.0
33	38.74760307809575	40.0	38.0	41.0	35.0	41.0
34	38.66572840739249	40.0	38.0	41.0	35.0	41.0
35	38.62665365210254	40.0	38.0	41.0	35.0	41.0
36	38.598438969141384	40.0	38.0	41.0	35.0	41.0
37	38.53843736820663	40.0	38.0	41.0	35.0	41.0
38	38.49629216342438	40.0	38.0	41.0	35.0	41.0
39	38.43235274088393	40.0	38.0	41.0	34.0	41.0
40	38.349209269651176	40.0	38.0	41.0	34.0	41.0
41	38.28936537273704	40.0	38.0	41.0	34.0	41.0
42	38.23238404274257	40.0	38.0	41.0	34.0	41.0
43	37.35655564861155	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	0.0
13	0.0
14	1.0
15	0.0
16	2.0
17	4.0
18	15.0
19	13.0
20	36.0
21	81.0
22	156.0
23	282.0
24	475.0
25	726.0
26	1132.0
27	1727.0
28	2405.0
29	3474.0
30	4811.0
31	6645.0
32	9015.0
33	12766.0
34	19480.0
35	30696.0
36	47034.0
37	85470.0
38	207643.0
39	402919.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.228583614791205	17.102045253885553	11.774994593858384	27.89437653746486
2	20.47440236747187	18.621858016202893	33.1388715321543	27.764868084170935
3	21.76781428201063	20.19674771299302	27.643125359164934	30.392312645831414
4	16.54852803607121	14.536845991271322	31.08095353585556	37.833672436801905
5	17.678501238334977	33.43528340726705	31.20580255217673	17.680412802221237
6	37.69508405504826	30.95395401016235	14.705302558747736	16.645659376041653
7	31.632320244297862	27.43966327802144	19.44633941489419	21.48167706278651
8	28.58863264640489	31.14116779827266	18.685775933649616	21.584423621672833
9	27.657701033797643	13.682257461371474	18.45125093935444	40.208790565476434
10	19.510735223312476	24.73217197862394	30.478094075227208	25.278998722836377
11	37.804162669307814	20.17775154687334	19.43737895917736	22.580706824641492
12	24.232059076881903	23.24401949317273	26.521515248903537	26.002406181041827
13	32.049877480702165	17.8174480383173	22.62383648482517	27.508837996155368
14	24.469331944263576	19.337858164349093	22.799222471389267	33.39358741999806
15	27.84216694882146	24.84722423002804	20.15672434412451	27.15388447702599
16	27.020672368702442	23.778779490353173	21.507722120736762	27.69282602020762
17	25.84255165105357	24.493465438327572	23.26301565929241	26.400967251326446
18	25.527143609821135	22.797908271217462	24.270887718321504	27.404060400639896
19	26.64803688362519	23.281414461697636	23.75930543326193	26.311243221415246
20	26.205987734928215	23.236014819399028	24.026685431852147	26.531312013820607
21	28.38481214703272	22.6381732139721	22.873773462953295	26.10324117604189
22	28.024721299959023	23.018693900080166	22.709856859706743	26.246727940254072
23	27.100719106439463	23.201009305731944	22.635664286371384	27.06260730145721
24	27.134290947191854	23.307459519647892	22.74856602840345	26.80968350475681
25	26.91231059090024	22.771504795038535	23.274604515352845	27.04158009870838
26	27.093550741865997	23.137330333771004	23.463968812835194	26.3051501115278
27	27.36989119617305	22.88691546467131	22.77437214086792	26.968821198287717
28	26.292724946267132	22.56935691406684	23.97220586109382	27.165712278572208
29	26.225820210248134	22.77532792281105	24.24567896957149	26.753172897369332
30	25.97719743229181	23.016423917965238	24.13456931868279	26.871809331060163
31	26.438242747108458	23.070784015980674	23.49419541678664	26.99677782012423
32	25.836458541166124	22.994201987787495	23.92871778268147	27.240621688364907
33	26.437525910651118	23.057283596033983	23.545090805258234	26.960099688056665
34	26.751619751711747	23.006029789333713	23.56145857103431	26.680891887920232
35	26.666316213287523	22.760513302692555	24.406847699731546	26.166322784288376
36	26.229284919791972	22.65728885283467	23.641146890542657	27.472279336830695
37	25.432282251965628	21.915960483195562	24.897044363813617	27.754712901025197
38	25.862384126373488	21.6049729334501	25.787116298352114	26.745526641824302
39	24.702542738386953	21.579525239214302	26.498815427754234	27.219116594644515
40	24.886650235182096	20.896738513591817	27.63165597584739	26.584955275378697
41	23.703631135074687	21.21202708208136	27.859132078311994	27.22520970453196
42	23.58391944669783	21.80449241407819	27.442889042079493	27.168699097144483
43	22.979626313154785	20.863047200096535	28.214563488412935	27.942762998335745
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	64.0
1	49.5
2	35.0
3	101.5
4	168.0
5	168.0
6	204.0
7	240.0
8	240.5
9	241.0
10	344.0
11	447.0
12	447.0
13	854.5
14	1262.0
15	1732.5
16	2203.0
17	2181.0
18	2159.0
19	2159.0
20	2728.5
21	3298.0
22	3333.5
23	3369.0
24	4103.5
25	4838.0
26	4838.0
27	5913.5
28	6989.0
29	8568.5
30	10148.0
31	12334.0
32	14520.0
33	14520.0
34	17402.5
35	20285.0
36	23498.5
37	26712.0
38	29291.0
39	31870.0
40	31870.0
41	34629.0
42	37388.0
43	40089.5
44	42791.0
45	44915.5
46	47040.0
47	47040.0
48	49108.5
49	51177.0
50	55144.0
51	59111.0
52	62562.0
53	66013.0
54	66013.0
55	66810.5
56	67608.0
57	65928.5
58	64249.0
59	60404.0
60	56559.0
61	56559.0
62	55833.0
63	55107.0
64	47149.0
65	39191.0
66	35262.0
67	31333.0
68	31333.0
69	27325.5
70	23318.0
71	21336.5
72	19355.0
73	19807.5
74	20260.0
75	20260.0
76	18522.5
77	16785.0
78	11429.0
79	6073.0
80	4155.5
81	2238.0
82	2238.0
83	1703.5
84	1169.0
85	956.5
86	744.0
87	573.5
88	403.0
89	403.0
90	292.5
91	182.0
92	111.5
93	41.0
94	30.5
95	20.0
96	20.0
97	13.0
98	6.0
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	837011.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.814615226335604
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.1107441919026	56.86567853138684
2	6.4703425570140904	8.25804841316877
3	1.6192696051344408	3.099992003980641
4	0.7269651896856179	1.855640154509111
5	0.3970376474552008	1.2668402351361563
6	0.2603213649142916	0.9967384642320012
7	0.1944295847627674	0.8685214398176544
8	0.14069719114373822	0.7182829693011077
9	0.11463398234895815	0.6583791127915181
>10	0.8034996606566863	10.107466311505224
>50	0.08797075851694595	3.864415989697111
>100	0.06789861383190039	8.382891137593496
>500	0.005626956938973117	2.541942128954603
>1k	5.626956938973117E-4	0.5151631079258188
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	2174	0.259733743045193	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1088	0.12998634426548755	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	1042	0.12449059809249818	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	969	0.11576908786144985	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	957	0.11433541494675697	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT	932	0.11134859637448014	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	918	0.10967597797400513	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	895	0.10692810488751044	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	886	0.10585285020149077	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	878	0.1048970682583622	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	855	0.10214919517186752	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	845	0.10095446774295677	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1947274289107312E-4	0.0
2	0.0	0.0	0.0	1.1947274289107312E-4	0.0
3	0.0	0.0	0.0	1.1947274289107312E-4	0.0
4	0.0	0.0	0.0	1.1947274289107312E-4	0.0
5	1.1947274289107312E-4	0.0	0.0	1.1947274289107312E-4	0.0
6	2.3894548578214624E-4	0.0	0.0	1.1947274289107312E-4	0.0
7	3.5841822867321935E-4	0.0	0.0	1.1947274289107312E-4	0.0
8	3.5841822867321935E-4	0.0	0.0	1.1947274289107312E-4	0.0
9	3.5841822867321935E-4	0.0	0.0	1.1947274289107312E-4	0.0
10	3.5841822867321935E-4	0.0	0.0	1.1947274289107312E-4	0.0
11	3.5841822867321935E-4	0.0	0.0	1.1947274289107312E-4	0.0
12	4.7789097156429247E-4	0.0	0.0	1.1947274289107312E-4	0.0
13	4.7789097156429247E-4	0.0	0.0	1.1947274289107312E-4	0.0
14	4.7789097156429247E-4	0.0	0.0	1.1947274289107312E-4	0.0
15	7.168364573464387E-4	0.0	0.0	2.3894548578214624E-4	0.0
16	7.168364573464387E-4	0.0	0.0	4.7789097156429247E-4	0.0
17	8.363092002375118E-4	0.0	0.0	5.973637144553656E-4	0.0
18	8.363092002375118E-4	1.1947274289107312E-4	0.0	7.168364573464387E-4	0.0
19	0.0011947274289107312	3.5841822867321935E-4	0.0	9.557819431285849E-4	0.0
20	0.0011947274289107312	3.5841822867321935E-4	0.0	0.001075254686019658	0.0
21	0.0011947274289107312	3.5841822867321935E-4	0.0	0.0014336729146928774	0.0
22	0.0011947274289107312	3.5841822867321935E-4	0.0	0.002150509372039316	0.0
23	0.0011947274289107312	3.5841822867321935E-4	0.0	0.002986818572276828	0.0
24	0.0013142001718018043	3.5841822867321935E-4	0.0	0.004898382458533998	0.0
25	0.0013142001718018043	3.5841822867321935E-4	0.0	0.007048891830573314	0.0
26	0.0013142001718018043	3.5841822867321935E-4	0.0	0.01338094720380019	0.0
27	0.0016726184004750237	3.5841822867321935E-4	0.0	0.04205440549765774	0.0
28	0.0019115638862571699	3.5841822867321935E-4	0.0	0.1261632164929732	0.0
29	0.0019115638862571699	3.5841822867321935E-4	0.0	0.22998503006531576	0.0
30	0.0019115638862571699	3.5841822867321935E-4	0.0	0.3622413564457337	0.0
31	0.0019115638862571699	3.5841822867321935E-4	0.0	0.6946145271686991	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTTA	25	0.0054955003	29.6	8
CCGTTAT	25	0.0054955003	29.6	9
TCGTATG	45	1.3227922E-4	24.666668	30
GGTATCA	720	0.0	22.611113	1
CTCGTAT	50	2.7012517E-4	22.2	29
CTACTAG	45	0.0038249518	20.555557	1
TCTCGTA	55	5.14149E-4	20.181818	28
GTATCAA	875	0.0	18.605715	2
CTAAACG	60	9.2339556E-4	18.5	26
CAGGACA	725	0.0	17.351725	4
TGCATCA	655	0.0	17.229008	14
ACAGGCT	735	0.0	17.115646	8
TATGCCG	65	0.0015794493	17.076923	33
TCGGAAC	120	1.0401709E-7	16.958334	32
GTTAACG	80	3.381191E-4	16.1875	13
GCTGCAT	705	0.0	16.007092	12
CTGCATC	705	0.0	16.007092	13
CCAGGAC	890	0.0	16.00562	3
GTTTTCG	105	9.336947E-6	15.857144	28
CGTATGC	70	0.002591704	15.857143	31
>>END_MODULE