FastQCFastQC Report
Fri 10 Feb 2017
ERR1632206.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632206.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257935
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT3960.15352705138891581No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2850.11049295365111364No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2630.1019636730183961No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCAT250.00548853929.65
TATATGC250.00548853929.63
GTTACAT250.00548853929.63
ATGACTT604.2955435E-827.74999821
GGTATCA1650.025.7878781
AATGTCT701.9109211E-723.78571329
AGACTGT603.7118803E-521.5833326
CTTATAC1301.8189894E-1221.34615337
AAGTGAG705.0766503E-621.1428577
TCAATGT806.917144E-720.812527
CTAGACT450.003817887320.5555554
TCTTATA2750.020.18181837
GCATTTT656.8756344E-519.92307713
GATGACT851.2376604E-619.58823620
GATTGGC851.2376604E-619.58823637
CAATGTC851.2376604E-619.58823628
ACTTCAA951.6635568E-719.47368424
TCGTTCG500.00702045618.537
TACCCTA609.210362E-418.55
GTTCTAG500.00702045618.51
Produced by FastQC (version 0.11.5)