##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632206.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	257935
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.633132378312364	31.0	30.0	31.0	26.0	34.0
2	29.867338670595306	31.0	30.0	31.0	26.0	34.0
3	29.907135518638416	31.0	30.0	31.0	26.0	34.0
4	33.52704363502433	35.0	33.0	37.0	28.0	37.0
5	31.763630371992942	35.0	30.0	37.0	22.0	37.0
6	31.770721305755327	35.0	30.0	36.0	23.0	37.0
7	31.739007889584585	35.0	30.0	35.0	23.0	37.0
8	31.631864617054685	35.0	30.0	35.0	21.0	37.0
9	32.79398685715393	35.0	30.0	38.0	23.0	39.0
10	32.32075135208483	35.0	30.0	37.0	19.0	39.0
11	32.63964564715917	35.0	30.0	38.0	23.0	39.0
12	32.45537441603505	35.0	30.0	37.0	21.0	39.0
13	32.66559792195708	35.0	30.0	37.0	23.0	39.0
14	33.1858607788784	36.0	31.0	38.0	23.0	40.0
15	33.16730959350224	36.0	31.0	38.0	23.0	40.0
16	33.10368891387365	36.0	31.0	38.0	22.0	40.0
17	33.05302110996956	36.0	31.0	38.0	21.0	40.0
18	33.047500339232755	36.0	31.0	38.0	21.0	40.0
19	33.04581774478066	36.0	30.0	39.0	20.0	40.0
20	32.98843507085118	36.0	30.0	39.0	19.0	40.0
21	32.91364878748522	36.0	30.0	39.0	19.0	40.0
22	32.83399306026712	36.0	30.0	39.0	19.0	40.0
23	32.72086378351135	36.0	30.0	39.0	19.0	40.0
24	32.57716478957877	35.0	30.0	39.0	19.0	40.0
25	32.434400139570045	35.0	30.0	38.0	18.0	40.0
26	32.144776784848894	35.0	29.0	38.0	18.0	40.0
27	31.9222943764902	35.0	29.0	38.0	17.0	40.0
28	31.75391474596313	35.0	28.0	38.0	17.0	40.0
29	31.574206679977515	35.0	27.0	38.0	16.0	40.0
30	31.370190164188653	34.0	27.0	38.0	16.0	40.0
31	31.239696047453815	34.0	27.0	38.0	16.0	40.0
32	31.019004788027992	34.0	27.0	38.0	16.0	40.0
33	30.861546513656542	34.0	27.0	38.0	15.0	40.0
34	30.718002597553646	34.0	26.0	38.0	15.0	40.0
35	30.66022447515847	34.0	26.0	38.0	15.0	40.0
36	30.54697113613895	34.0	26.0	38.0	15.0	40.0
37	30.417322193575902	33.0	26.0	38.0	15.0	40.0
38	30.300052338767518	33.0	25.0	38.0	15.0	40.0
39	30.218632601236745	33.0	25.0	38.0	14.0	40.0
40	30.01755481032043	33.0	25.0	38.0	13.0	40.0
41	29.927198712854015	33.0	25.0	38.0	13.0	40.0
42	29.792416694128367	33.0	25.0	38.0	13.0	40.0
43	28.77452071258263	32.0	24.0	37.0	9.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	9.0
11	23.0
12	53.0
13	118.0
14	265.0
15	458.0
16	730.0
17	1076.0
18	1538.0
19	2170.0
20	2939.0
21	3812.0
22	4845.0
23	5699.0
24	7115.0
25	8409.0
26	9874.0
27	11292.0
28	12728.0
29	14307.0
30	15833.0
31	17242.0
32	18184.0
33	19476.0
34	20092.0
35	19867.0
36	19214.0
37	17319.0
38	15529.0
39	7718.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.26402000504003	21.48758408126078	13.99383565626999	24.254560257429198
2	22.065636691414504	24.40963808711497	29.709035222052066	23.815689999418456
3	23.031771570356874	23.906022835210422	28.222614224513926	24.83959136991878
4	19.560742047415047	17.600170585612656	30.800007753891485	32.039079613080816
5	17.151607963246555	36.09048791362165	30.92988543625332	15.828018686878478
6	36.55843526469847	34.63314400914959	13.971349370965552	14.837071355186385
7	31.41993137806036	30.029270940353193	19.23236474305542	19.318432938531025
8	29.007695737298157	32.77841316610774	18.682613836819353	19.531277259774747
9	28.33892259677826	13.951576947680616	18.180937057785876	39.52856339775525
10	18.198771008199742	27.32006125574273	30.948882470389826	23.532285265667706
11	39.543295791575396	20.721887297187276	19.36418089828833	20.370636012949
12	23.907573613507278	25.15633783705197	27.00990559637118	23.92618295306957
13	32.92302324228972	18.55467462732859	23.09186422936011	25.430437901021573
14	25.05398646945936	19.81584507724814	22.90111849884661	32.229049954445884
15	27.62129993990734	27.10799232364743	19.91431949909861	25.356388237346618
16	27.545699497935527	25.245119894547074	21.876054044623647	25.333126562893753
17	26.2422703394266	25.60645123771493	22.648729331033014	25.50254909182546
18	26.582666175586873	23.974644774846375	24.130110299106363	25.312578750460386
19	26.91181886909493	24.30108360633493	23.86841646151162	24.91868106305852
20	27.561982670052533	23.871130323531123	23.486925000484618	25.079962005931726
21	28.16174617636226	24.361176265338166	23.154283055808634	24.32279450249094
22	28.08110570492566	24.269292651249348	22.893752301936534	24.75584934188846
23	27.000213232015817	24.410801170837615	23.23957586213581	25.34940973501076
24	26.964157636613876	24.38443793979103	23.224068079167232	25.427336344427857
25	27.179328125302888	23.80289607846938	23.62959660379553	25.388179192432204
26	27.361932269757883	24.02388198577161	22.922829395002616	25.69135634946789
27	26.86490782561498	24.091728536259136	23.56834086106965	25.475022777056232
28	26.59584779111016	23.824219280051175	24.465078411227633	25.114854517611025
29	26.782716575881523	23.868804156085837	23.823831585476963	25.524647682555685
30	26.44619768546339	24.58216217264039	23.869579545234267	25.10206059666195
31	27.398763254308257	24.253397173706553	23.132572159652625	25.215267412332565
32	26.784655048752594	23.89981972202299	23.334561032818346	25.98096419640607
33	26.44697307461182	24.168879756527808	24.07544536414213	25.308701804718243
34	27.586407428228043	23.412487642235448	23.669529144939617	25.331575784596893
35	26.783879659604164	23.99519258727974	24.318142167600364	24.90278558551573
36	27.084730649194565	23.224068079167232	24.549208133832167	25.141993137806036
37	26.80171361001803	23.24577897532324	24.53718960203152	25.415317812627215
38	26.262042762711534	22.95384496093977	25.202473491383486	25.581638784965204
39	27.03355496539826	22.837924283249656	25.109814488146238	25.018706263205846
40	26.137205109814488	23.50824820206641	25.355225153623973	24.999321534495124
41	26.512105763079845	22.616162986798997	25.636691414503655	25.2350398356175
42	26.372148021788433	22.75340686607091	25.812317056622792	25.062128055517864
43	25.24977222943765	22.241650028107856	26.18450384786865	26.324073894585847
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	7.0
2	7.0
3	23.0
4	39.0
5	39.0
6	53.0
7	67.0
8	63.0
9	59.0
10	70.5
11	82.0
12	82.0
13	165.0
14	248.0
15	386.0
16	524.0
17	521.0
18	518.0
19	518.0
20	603.5
21	689.0
22	654.0
23	619.0
24	784.0
25	949.0
26	949.0
27	1243.0
28	1537.0
29	1995.5
30	2454.0
31	3005.0
32	3556.0
33	3556.0
34	4619.0
35	5682.0
36	7171.5
37	8661.0
38	9562.0
39	10463.0
40	10463.0
41	11830.0
42	13197.0
43	14488.5
44	15780.0
45	17407.5
46	19035.0
47	19035.0
48	20043.0
49	21051.0
50	21799.5
51	22548.0
52	22580.0
53	22612.0
54	22612.0
55	22014.5
56	21417.0
57	20886.0
58	20355.0
59	19057.0
60	17759.0
61	17759.0
62	16328.0
63	14897.0
64	13146.5
65	11396.0
66	9906.0
67	8416.0
68	8416.0
69	6879.5
70	5343.0
71	4499.5
72	3656.0
73	2814.5
74	1973.0
75	1973.0
76	1490.0
77	1007.0
78	808.5
79	610.0
80	478.0
81	346.0
82	346.0
83	265.5
84	185.0
85	139.5
86	94.0
87	79.5
88	65.0
89	65.0
90	43.5
91	22.0
92	14.5
93	7.0
94	5.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	257935.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.19736630655005
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.09043199701462	67.58670149904381
2	4.698814393040704	6.972793054538615
3	1.5151426126365082	3.3725877430936273
4	0.737172208047243	2.187849454059585
5	0.4230804258168754	1.569572666573294
6	0.28708728247529514	1.2780672155862889
7	0.1790393559785166	0.9298974075178768
8	0.15303488331985216	0.9083828236290572
9	0.10390283155892642	0.6938384808118889
>10	0.7499850879268606	10.476219758376496
>50	0.04764799931760897	2.3683000589598895
>100	0.014660922866956605	1.6557898378095552
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	396	0.15352705138891581	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	285	0.11049295365111364	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	263	0.1019636730183961	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	3.876945742144339E-4	0.0	0.0	0.0
5	0.0	3.876945742144339E-4	0.0	0.0	0.0
6	0.0	3.876945742144339E-4	0.0	0.0	0.0
7	0.0	3.876945742144339E-4	0.0	0.0	0.0
8	0.0	3.876945742144339E-4	0.0	0.0	0.0
9	0.0	3.876945742144339E-4	0.0	0.0	0.0
10	0.0	3.876945742144339E-4	0.0	0.0	0.0
11	0.0	3.876945742144339E-4	0.0	0.0	0.0
12	0.0	3.876945742144339E-4	0.0	0.0	0.0
13	0.0	3.876945742144339E-4	0.0	0.0	0.0
14	0.0	3.876945742144339E-4	0.0	0.0	0.0
15	0.0	3.876945742144339E-4	0.0	0.0	0.0
16	0.0	3.876945742144339E-4	0.0	0.0	0.0
17	0.0	3.876945742144339E-4	0.0	0.0	0.0
18	0.0	3.876945742144339E-4	0.0	0.0	0.0
19	0.0	3.876945742144339E-4	0.0	0.0	0.0
20	0.0	3.876945742144339E-4	0.0	3.876945742144339E-4	0.0
21	0.0	3.876945742144339E-4	0.0	3.876945742144339E-4	0.0
22	0.0	3.876945742144339E-4	0.0	3.876945742144339E-4	0.0
23	0.0	3.876945742144339E-4	0.0	3.876945742144339E-4	0.0
24	0.0	3.876945742144339E-4	0.0	7.753891484288677E-4	0.0
25	0.0	3.876945742144339E-4	0.0	0.0011630837226433016	0.0
26	0.0	3.876945742144339E-4	0.0	0.0019384728710721694	0.0
27	0.0	3.876945742144339E-4	0.0	0.00697850233585981	0.0
28	0.0	3.876945742144339E-4	0.0	0.032566344234012445	0.0
29	0.0	3.876945742144339E-4	0.0	0.06823424506174036	0.0
30	0.0	3.876945742144339E-4	0.0	0.11940992885804563	0.0
31	0.0	3.876945742144339E-4	0.0	0.32954038808226876	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATCAT	25	0.005488539	29.6	5
TATATGC	25	0.005488539	29.6	3
GTTACAT	25	0.005488539	29.6	3
ATGACTT	60	4.2955435E-8	27.749998	21
GGTATCA	165	0.0	25.787878	1
AATGTCT	70	1.9109211E-7	23.785713	29
AGACTGT	60	3.7118803E-5	21.583332	6
CTTATAC	130	1.8189894E-12	21.346153	37
AAGTGAG	70	5.0766503E-6	21.142857	7
TCAATGT	80	6.917144E-7	20.8125	27
CTAGACT	45	0.0038178873	20.555555	4
TCTTATA	275	0.0	20.181818	37
GCATTTT	65	6.8756344E-5	19.923077	13
GATGACT	85	1.2376604E-6	19.588236	20
GATTGGC	85	1.2376604E-6	19.588236	37
CAATGTC	85	1.2376604E-6	19.588236	28
ACTTCAA	95	1.6635568E-7	19.473684	24
TCGTTCG	50	0.007020456	18.5	37
TACCCTA	60	9.210362E-4	18.5	5
GTTCTAG	50	0.007020456	18.5	1
>>END_MODULE