FastQCFastQC Report
Fri 10 Feb 2017
ERR1632205.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632205.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43308
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2340.5403158769742311No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1690.390228133370278No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT1540.3555925002309042TruSeq Adapter, Index 12 (95% over 22bp)
TCAGAGCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGC1320.30479357162648935No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT1240.28632123395215664No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1200.2770850651149903No Hit
ATTCAGAGCACTGGGCAGAAATCACATCGCGTCAACACCCGCC1030.23783134755703336No Hit
CGTCAACACCCGCCGCGGGCCTTCGCGATGCTTTGTTTTAATT750.17317816569686895No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.17086912348757735No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA660.15239678581324467No Hit
ACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAATTA660.15239678581324467No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC650.15008774360395308No Hit
GGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCGC610.14085157476678675No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT570.1316154059296204No Hit
GGTATCAACGCAGAGTACATGGGACTCCCGACCCGGGGAGGTA560.1293063637203288No Hit
GAATCCGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGC560.1293063637203288No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC540.12468827930174563No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC540.12468827930174563No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC520.12007019488316245No Hit
GCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCC470.10852498383670454No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGC470.10852498383670454No Hit
AAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATG470.10852498383670454No Hit
GCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGATTTCTGCCC460.10621594162741295No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTGT250.00543884829.59999835
GGTATCA1600.028.906251
TCTGTCG451.2947142E-424.6666688
GTCGCCC450.00376754620.55555711
TCGCTCT450.00376754620.5555574
ACCTCTG450.00376754620.55555737
GTATCAA2300.020.1086962
GTCTCAC656.708218E-519.9230771
TCTCACT656.708218E-519.9230772
TGTCGCC609.042659E-418.510
AACCTCT500.006928773618.49999836
CTTATAC852.6265185E-517.41176437
CTGTCGC650.001546993517.0769239
GCCCAGG1151.1760967E-616.08695614
ACTCCAG700.002538882715.85714332
CCCAGGC1701.3387762E-915.23529415
GCTCTGT750.004020225714.86
TCTTATA1001.0553038E-414.79999936
TATCAAC3200.014.4531253
ATCAACG3450.013.4057974
Produced by FastQC (version 0.11.5)