##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632205.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	43308
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43186016440381	33.0	31.0	34.0	31.0	34.0
2	32.57924632862289	34.0	31.0	34.0	31.0	34.0
3	32.611457467442506	34.0	31.0	34.0	31.0	34.0
4	36.16541978387365	37.0	35.0	37.0	35.0	37.0
5	36.0039022813337	37.0	35.0	37.0	35.0	37.0
6	36.04412579661956	37.0	35.0	37.0	35.0	37.0
7	36.021820448877804	37.0	35.0	37.0	35.0	37.0
8	36.00480280779533	37.0	35.0	37.0	35.0	37.0
9	37.73485268310705	39.0	37.0	39.0	35.0	39.0
10	37.64073612265632	39.0	37.0	39.0	35.0	39.0
11	37.77034266186386	39.0	37.0	39.0	35.0	39.0
12	37.680359286967764	39.0	37.0	39.0	35.0	39.0
13	37.71554909023737	39.0	37.0	39.0	35.0	39.0
14	38.89325297866445	40.0	38.0	41.0	36.0	41.0
15	38.91585850189342	40.0	38.0	41.0	36.0	41.0
16	38.86090329731228	40.0	38.0	41.0	36.0	41.0
17	38.89239863304701	40.0	38.0	41.0	36.0	41.0
18	38.92458668144454	40.0	38.0	41.0	36.0	41.0
19	38.90456728548998	40.0	38.0	41.0	36.0	41.0
20	38.91675902835504	40.0	38.0	41.0	35.0	41.0
21	38.84439364551584	40.0	38.0	41.0	35.0	41.0
22	38.813775745820635	40.0	38.0	41.0	35.0	41.0
23	38.760021243188326	40.0	38.0	41.0	35.0	41.0
24	38.66724392721899	40.0	38.0	41.0	35.0	41.0
25	38.607208829777406	40.0	38.0	41.0	35.0	41.0
26	38.50759674886857	40.0	38.0	41.0	35.0	41.0
27	38.40274314214464	40.0	38.0	41.0	34.0	41.0
28	38.34734921954373	40.0	38.0	41.0	34.0	41.0
29	38.19811582155722	40.0	38.0	41.0	34.0	41.0
30	38.137272559342385	40.0	38.0	41.0	34.0	41.0
31	37.96529509559435	40.0	37.0	41.0	33.0	41.0
32	37.85369908561928	40.0	37.0	41.0	33.0	41.0
33	37.75371755795696	40.0	37.0	41.0	33.0	41.0
34	37.662210215202734	40.0	37.0	41.0	33.0	41.0
35	37.59559434746467	40.0	37.0	41.0	33.0	41.0
36	37.47813337027801	40.0	36.0	41.0	33.0	41.0
37	37.40479818971091	40.0	36.0	41.0	33.0	41.0
38	37.36993165235061	40.0	36.0	41.0	33.0	41.0
39	37.27265170407315	39.0	36.0	41.0	32.0	41.0
40	37.13960469197377	39.0	35.0	41.0	32.0	41.0
41	37.06172069825436	39.0	35.0	41.0	32.0	41.0
42	36.942227763923526	39.0	35.0	41.0	31.0	41.0
43	36.023252055047564	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	1.0
17	2.0
18	2.0
19	3.0
20	9.0
21	9.0
22	12.0
23	39.0
24	59.0
25	80.0
26	128.0
27	200.0
28	248.0
29	363.0
30	451.0
31	637.0
32	780.0
33	1066.0
34	1602.0
35	2319.0
36	3605.0
37	6440.0
38	12714.0
39	12538.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.001293063637206	19.643022074443518	14.452295187956036	22.90338967396324
2	20.973030386995475	21.554909023736954	31.77242079985222	25.69963978941535
3	21.831994088851943	22.08829777408331	30.88574859148425	25.19395954558049
4	15.837720513530988	16.29491087097072	30.802623071949753	37.06474554354854
5	16.091715156553065	35.14824050983652	29.521104645792924	19.238939687817496
6	34.81804747390782	31.211323542994368	15.248914750161632	18.72171423293618
7	27.710815553708322	31.51842615683015	18.37535790154244	22.39540038791909
8	30.139927957883074	30.34081463009144	19.01958067793479	20.4996767340907
9	27.74776022905699	12.115544472152951	19.486007204211692	40.65068809457837
10	19.91779809734922	26.6186385887134	29.207074905329268	24.25648840860811
11	36.77842430959638	20.114066685139004	16.779809734921955	26.327699270342663
12	24.40195806779348	28.29038514824051	24.584372402327514	22.723284381638496
13	35.50845109448601	17.904313290846957	22.21529509559435	24.371940519072687
14	26.26766417290108	20.698254364089774	23.843169853144914	29.19091160986423
15	30.982728364274497	24.392721898956314	20.257227302115083	24.367322434654106
16	28.029463378590563	23.12274868384594	22.23376743326868	26.614020504294817
17	24.40195806779348	27.28595178719867	22.62861365105754	25.68347649395031
18	25.632677565345897	21.919737692805025	25.11776115267387	27.329823589175213
19	27.63230811859241	24.281887872910314	24.51971922046735	23.566084788029926
20	29.174748314399185	19.839290662233307	26.223792370924542	24.762168652442966
21	30.41932206520735	21.180844185831717	22.53856100489517	25.86127274406576
22	29.09393183707398	22.330747206058927	24.175671931282906	24.39964902558419
23	28.17262399556664	21.894338228502814	22.778701394661493	27.15433638126905
24	26.747944952433734	23.39752470675164	24.623626119885472	25.23090422092916
25	26.15913918906438	22.337674332686802	23.990948554539575	27.512237923709243
26	26.581693913364735	22.212986053385055	23.54299436593701	27.662325667313198
27	25.477971737323358	23.09042209291586	24.66749792186201	26.764108247898772
28	26.216865244296667	21.94513715710723	23.882423570702873	27.95557402789323
29	25.20550475662695	22.32151103722176	25.9051445460423	26.567839660108987
30	25.260921769649947	22.446199316523508	26.085249838367048	26.207629075459497
31	26.357716819063455	22.56626951140667	23.406760875588805	27.669252793941073
32	24.078692158492657	23.155075274776024	25.90283550383301	26.863397062898308
33	24.274960746282442	22.046735014316063	26.325390228133372	27.352914011268126
34	24.99769095779071	22.01209938117669	27.018102890920847	25.972106770111758
35	25.586496721160064	22.686339706289832	26.25380991964533	25.473353652904773
36	25.055417013022996	22.94957051814907	24.787568116745174	27.207444352082753
37	23.376743326868016	22.968042855823402	25.981342938948927	27.673870878359658
38	23.80391613558696	21.47871063083033	27.38524060219821	27.332132631384503
39	23.963240048028077	21.57799944582987	27.974046365567563	26.48471414057449
40	23.549921492564884	20.839105938856562	28.837628151842615	26.773344416735938
41	23.187401865706107	20.917613373972475	28.77297496998245	27.122009790338968
42	22.390782303500508	20.87143252978664	29.89286044148887	26.84492472522398
43	20.693636279671193	21.090791539669347	29.73815461346633	28.47741756719313
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	6.0
2	9.0
3	17.0
4	25.0
5	25.0
6	29.5
7	34.0
8	37.0
9	40.0
10	57.0
11	74.0
12	74.0
13	127.0
14	180.0
15	267.5
16	355.0
17	299.5
18	244.0
19	244.0
20	319.5
21	395.0
22	297.0
23	199.0
24	221.0
25	243.0
26	243.0
27	303.5
28	364.0
29	439.5
30	515.0
31	608.0
32	701.0
33	701.0
34	799.0
35	897.0
36	1087.0
37	1277.0
38	1329.0
39	1381.0
40	1381.0
41	1538.0
42	1695.0
43	1857.0
44	2019.0
45	2225.0
46	2431.0
47	2431.0
48	2607.0
49	2783.0
50	2950.0
51	3117.0
52	3178.5
53	3240.0
54	3240.0
55	3614.5
56	3989.0
57	3890.0
58	3791.0
59	3822.5
60	3854.0
61	3854.0
62	3360.0
63	2866.0
64	2526.0
65	2186.0
66	1881.0
67	1576.0
68	1576.0
69	1276.5
70	977.0
71	787.0
72	597.0
73	496.0
74	395.0
75	395.0
76	320.5
77	246.0
78	228.0
79	210.0
80	188.0
81	166.0
82	166.0
83	143.5
84	121.0
85	87.0
86	53.0
87	41.0
88	29.0
89	29.0
90	22.5
91	16.0
92	13.0
93	10.0
94	6.5
95	3.0
96	3.0
97	2.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	43308.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.95021704996768
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.25206390352484	77.23977094301284
2	3.282522329604688	5.380068347649395
3	0.9072722662083345	2.230534774175672
4	0.4113718971006734	1.3484806502262863
5	0.2000507170832042	0.8197099842985129
6	0.20850356428390296	1.0252147409254642
7	0.1296103237440478	0.7435115913918907
8	0.10143416640838523	0.6650041562759768
9	0.05635231467132513	0.41562759767248547
>10	0.39728381843284216	6.040454419506788
>50	0.03381138880279507	1.6994550660386072
>100	0.019723310134963794	2.392167728826083
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	234	0.5403158769742311	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	169	0.390228133370278	No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	154	0.3555925002309042	TruSeq Adapter, Index 12 (95% over 22bp)
TCAGAGCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGC	132	0.30479357162648935	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	124	0.28632123395215664	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	120	0.2770850651149903	No Hit
ATTCAGAGCACTGGGCAGAAATCACATCGCGTCAACACCCGCC	103	0.23783134755703336	No Hit
CGTCAACACCCGCCGCGGGCCTTCGCGATGCTTTGTTTTAATT	75	0.17317816569686895	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	74	0.17086912348757735	No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA	66	0.15239678581324467	No Hit
ACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAATTA	66	0.15239678581324467	No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC	65	0.15008774360395308	No Hit
GGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCGC	61	0.14085157476678675	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT	57	0.1316154059296204	No Hit
GGTATCAACGCAGAGTACATGGGACTCCCGACCCGGGGAGGTA	56	0.1293063637203288	No Hit
GAATCCGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGC	56	0.1293063637203288	No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC	54	0.12468827930174563	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC	54	0.12468827930174563	No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC	52	0.12007019488316245	No Hit
GCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCC	47	0.10852498383670454	No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGC	47	0.10852498383670454	No Hit
AAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATG	47	0.10852498383670454	No Hit
GCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGATTTCTGCCC	46	0.10621594162741295	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0023090422092915857	0.0
13	0.0	0.0	0.0	0.0023090422092915857	0.0
14	0.0	0.0	0.0	0.0023090422092915857	0.0
15	0.0	0.0	0.0	0.0023090422092915857	0.0
16	0.0	0.0	0.0	0.0023090422092915857	0.0
17	0.0	0.0	0.0	0.004618084418583171	0.0
18	0.0	0.0	0.0	0.004618084418583171	0.0
19	0.0	0.0	0.0	0.004618084418583171	0.0
20	0.0	0.0	0.0	0.004618084418583171	0.0
21	0.0	0.0	0.0	0.01154521104645793	0.0
22	0.0	0.0	0.0	0.025399464302207443	0.0
23	0.0	0.0	0.0	0.02770850651149903	0.0
24	0.0	0.0	0.0	0.04156275976724855	0.0
25	0.0	0.0	0.0	0.04156275976724855	0.0
26	0.0	0.0	0.0	0.050798928604414886	0.0
27	0.0	0.0	0.0	0.11083402604599613	0.0
28	0.0	0.0	0.0	0.2101228410455343	0.0
29	0.0	0.0	0.0	0.35097441581232103	0.0
30	0.0	0.0	0.0	0.551861088020689	0.0
31	0.0	0.0	0.0	0.9674886856931745	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCCTGT	25	0.005438848	29.599998	35
GGTATCA	160	0.0	28.90625	1
TCTGTCG	45	1.2947142E-4	24.666668	8
GTCGCCC	45	0.003767546	20.555557	11
TCGCTCT	45	0.003767546	20.555557	4
ACCTCTG	45	0.003767546	20.555557	37
GTATCAA	230	0.0	20.108696	2
GTCTCAC	65	6.708218E-5	19.923077	1
TCTCACT	65	6.708218E-5	19.923077	2
TGTCGCC	60	9.042659E-4	18.5	10
AACCTCT	50	0.0069287736	18.499998	36
CTTATAC	85	2.6265185E-5	17.411764	37
CTGTCGC	65	0.0015469935	17.076923	9
GCCCAGG	115	1.1760967E-6	16.086956	14
ACTCCAG	70	0.0025388827	15.857143	32
CCCAGGC	170	1.3387762E-9	15.235294	15
GCTCTGT	75	0.0040202257	14.8	6
TCTTATA	100	1.0553038E-4	14.799999	36
TATCAAC	320	0.0	14.453125	3
ATCAACG	345	0.0	13.405797	4
>>END_MODULE