FastQCFastQC Report
Fri 10 Feb 2017
ERR1632204.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632204.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44513
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG1530.3437198121896974No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG1330.2987891177858154No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA1260.28306337474445664No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC1160.26059802754251565No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT1110.2493653539415452No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT1110.2493653539415452No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC1080.24262574978096288No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG1050.23588614562038054No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC940.21117426369824543No Hit
GGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGATCTGG870.19544852065688675No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC850.19095545121649854No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT850.19095545121649854RNA PCR Primer, Index 17 (95% over 21bp)
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA830.18646238177611035No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT810.18196931233572214No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA770.17298317345494577No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC760.17073663873475164No Hit
ATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAGGGCAC740.16624356929436343No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT720.16175049985397524No Hit
GTCTTGAAGGGAACGTTGCCAGCTGCCTTGTACCAGCATTACA720.16175049985397524No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA710.15950396513378115No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA700.15725743041358703No Hit
CATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTCTTCACCTGCT700.15725743041358703No Hit
GTCCAGATACTTGCTGTAGTCACTGGTGAATGTGCCCTGTGAA680.15276436097319884No Hit
GGTGAATGTGCCCTGTGAATGGCGCTTGTCCTCGTTCATCTGA660.14827129153281066No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG650.14602475681261656No Hit
CTTCAGCATGTCTCTCAAATTCATCGTGACGTTTGGCAATGTT640.14377822209242244No Hit
GCCTGGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTT600.13479208321164604No Hit
ATTCTCAGCTTCCCAGGCAGACCCACTCAGTGATCCTGATCAG580.13029901377125783No Hit
TGCTGGTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGA570.12805247905106373No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC570.12805247905106373No Hit
GTGTTCATCTCATCAGAGAAAGAACCATCAGCATGTCTGCGGC560.12580594433086964No Hit
GCCTTGTACCAGCATTACAAATAATCCAGCCACAAAGTAAATG560.12580594433086964No Hit
ATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAGAT550.12355940961067553No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC520.11681980545009323No Hit
TTCCCAGGCAGACCCACTCAGTGATCCTGATCAGATGAACGAG510.11457327072989913No Hit
GTGAATGGCGCTTGTCCTCGTTCATCTGATCAGGATCACTGAG500.11232673600970504No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC490.11008020128951093No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA490.11008020128951093No Hit
CTGGTGAATGTGCCCTGTGAATGGCGCTTGTCCTCGTTCATCT490.11008020128951093No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT490.11008020128951093No Hit
GATCAGGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGA490.11008020128951093No Hit
GAGGAGAAATCCAGATCATTCTCAGCTTCCCAGGCAGACCCAC480.10783366656931684No Hit
TTGCTGTAGTCACTGGTGAATGTGCCCTGTGAATGGCGCTTGT480.10783366656931684No Hit
GGTGTATTCTGAGGCCACATTGCTTTGCATGCCAATAAATAAA470.10558713184912272No Hit
GTAAATGCTTTTCATTTCTGCTGTCTTCTGGTAGTGTGCTGTA470.10558713184912272No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG460.10334059712892862No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC460.10334059712892862No Hit
GGACTTAACATTTCAAACATCCCACGTGGCTAGCAGGTGATGT460.10334059712892862No Hit
ATCCAGATCATTCTCAGCTTCCCAGGCAGACCCACTCAGTGAT460.10334059712892862No Hit
CTTCTGGTAGTGTGCTGTAGAACAGAGCAGGTGAAGAGAGAGC450.10109406240873453No Hit
GAGTGGGTCTGCCTGGGAAGCTGAGAATGATCTGGATTTCTCC450.10109406240873453No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA200.001822990137.01
TTGAAGA352.3374936E-531.71428522
GTCGCCA303.5446536E-430.83333412
TGTTGAA405.814752E-527.7520
CATTGTT405.814752E-527.7517
AGAAGAG405.814752E-527.755
ATTGTTG405.814752E-527.7518
GGACACA358.7355723E-426.428576
CTTAGGA358.7355723E-426.428572
TCGCCAT358.7355723E-426.4285713
TTAGGAC358.7355723E-426.428573
AGCACAT358.7355723E-426.4285714
GCTTAGG358.7355723E-426.428571
GAAGAGG508.83748E-625.9000026
GTTGAAG451.2955071E-424.66666421
CCATTGT451.2955071E-424.66666416
GCCATTG451.2955071E-424.66666415
TAGGACA400.001902324123.1254
AGAGGTC400.001902324123.1258
GAGCACA400.001902324123.12513
Produced by FastQC (version 0.11.5)