##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632204.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	44513
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.91139667063555	34.0	31.0	34.0	31.0	34.0
2	33.07804461617954	34.0	33.0	34.0	31.0	34.0
3	33.14433985577247	34.0	33.0	34.0	31.0	34.0
4	36.498528519758274	37.0	37.0	37.0	35.0	37.0
5	36.37535102105003	37.0	37.0	37.0	35.0	37.0
6	36.41684451733202	37.0	37.0	37.0	35.0	37.0
7	36.40833015074248	37.0	37.0	37.0	35.0	37.0
8	36.389302001662436	37.0	37.0	37.0	35.0	37.0
9	38.19169680767416	39.0	39.0	39.0	37.0	39.0
10	38.18536157976322	39.0	39.0	39.0	37.0	39.0
11	38.25046615595444	39.0	39.0	39.0	37.0	39.0
12	38.23159526430481	39.0	39.0	39.0	37.0	39.0
13	38.237705838743736	39.0	39.0	39.0	37.0	39.0
14	39.69741423853706	41.0	39.0	41.0	38.0	41.0
15	39.719767259002985	41.0	40.0	41.0	38.0	41.0
16	39.71100577359423	41.0	39.0	41.0	38.0	41.0
17	39.69381978298475	41.0	39.0	41.0	38.0	41.0
18	39.66414305933098	41.0	39.0	41.0	37.0	41.0
19	39.715566239076225	41.0	40.0	41.0	38.0	41.0
20	39.70871430817963	41.0	40.0	41.0	38.0	41.0
21	39.66041381169546	41.0	40.0	41.0	37.0	41.0
22	39.654999663019794	41.0	39.0	41.0	37.0	41.0
23	39.602251027789634	41.0	39.0	41.0	37.0	41.0
24	39.565048412823224	41.0	39.0	41.0	37.0	41.0
25	39.532024352436366	41.0	39.0	41.0	37.0	41.0
26	39.45449643924247	41.0	39.0	41.0	37.0	41.0
27	39.40570170511985	41.0	39.0	41.0	37.0	41.0
28	39.365331476197966	41.0	39.0	41.0	37.0	41.0
29	39.29986745445151	40.0	39.0	41.0	36.0	41.0
30	39.261631433513806	40.0	39.0	41.0	36.0	41.0
31	39.18940534225956	40.0	39.0	41.0	36.0	41.0
32	39.10446386448903	40.0	39.0	41.0	36.0	41.0
33	39.038685327881744	40.0	39.0	41.0	35.0	41.0
34	39.005751128883695	40.0	39.0	41.0	35.0	41.0
35	38.981084177655966	40.0	39.0	41.0	35.0	41.0
36	38.925999146316805	40.0	39.0	41.0	35.0	41.0
37	38.90941972008177	40.0	39.0	41.0	35.0	41.0
38	38.855794936310744	40.0	38.0	41.0	35.0	41.0
39	38.834340529732884	40.0	38.0	41.0	35.0	41.0
40	38.80756183586817	40.0	38.0	41.0	35.0	41.0
41	38.76811268618157	40.0	38.0	41.0	35.0	41.0
42	38.735919843641184	40.0	38.0	41.0	35.0	41.0
43	37.91905735403141	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	0.0
15	0.0
16	0.0
17	0.0
18	1.0
19	0.0
20	1.0
21	3.0
22	6.0
23	13.0
24	26.0
25	29.0
26	37.0
27	68.0
28	102.0
29	139.0
30	183.0
31	289.0
32	386.0
33	562.0
34	829.0
35	1256.0
36	2134.0
37	3888.0
38	10515.0
39	24044.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.945049760744055	18.02394806011727	13.569069709972368	26.46193246916631
2	19.441511468559746	21.297149147440074	34.27987329544178	24.9814660885584
3	20.73102239795116	21.90371352189248	30.07436029923842	27.290903780917937
4	16.381731179655382	16.038011367465685	31.04710983308247	36.533147619796466
5	15.954889582818502	35.306539662570486	32.99260890077056	15.745961853840452
6	35.713162446925615	32.336620762473885	15.402242041650755	16.547974748949745
7	29.74636623009009	29.5509177094332	20.12895109293914	20.57376496753757
8	26.183362163862245	33.46662772673152	20.234538224788263	20.11547188461798
9	26.270977017949814	15.14389054882843	18.79675600386404	39.78837642935771
10	17.93184013658931	27.495338440455598	31.548087075685753	23.024734347269337
11	35.40538719025903	21.908206591332867	20.620942196661648	22.065464021746457
12	21.75544223035967	25.145463123132565	26.47316514276728	26.62592950374048
13	30.060881090917256	19.122503538292186	24.873632421989083	25.942982948801475
14	23.89189674926426	21.034304585177363	22.211488778559072	32.862309886999306
15	26.994361197852314	27.185316649068813	20.962415474131152	24.85790667894772
16	27.313369128119874	25.664412643497403	22.224967986880237	24.797250241502482
17	25.59926313661178	26.356345337317187	22.838271965493227	25.20611956057781
18	23.429110596904277	24.74557994293802	25.410554220115472	26.414755240042236
19	26.02610473344866	25.41954035899625	24.77253835958035	23.78181654797475
20	25.603756206052164	23.77058387437378	25.718329476782063	24.907330442791995
21	25.455484914519356	25.012917574641115	23.505492777390874	26.02610473344866
22	25.790218587828274	23.456069013546603	23.72789971469009	27.02581268393503
23	24.246849235054928	25.778985914227302	24.296272998899198	25.67789185181857
24	26.713544357828052	24.7141284568553	23.844719520140185	24.727607665176464
25	26.34286612899602	24.590569047244625	23.99523734639319	25.07132747736616
26	26.601217621818346	24.39961359602813	23.61332644396019	25.38584233819334
27	25.835149282232155	23.660503673084268	23.970525464471056	26.533821580212525
28	24.65347201941006	23.768337339653584	25.233077977220137	26.34511266371622
29	24.747826477658215	25.138723518971982	24.04915417967785	26.064295823691957
30	24.282793790578033	26.286702760991172	24.287286860018423	25.143216588412376
31	26.0463235459304	23.905375957585427	24.76804529013996	25.280255206344215
32	23.85819872846135	24.12104329072406	25.659919574057017	26.360838406757576
33	24.947768067755486	23.700941298047763	24.907330442791995	26.44396019140476
34	25.466717588120325	24.224383887852987	24.743333408217826	25.565565115808862
35	25.31619976186732	23.977265068631638	24.900590838631413	25.805944330869636
36	24.309752207220363	24.58158290836385	25.271269067463436	25.837395816952352
37	26.21930671938535	22.119380855031114	24.660211623570643	27.001100802012896
38	24.40635320018871	23.3055511872936	27.077482982499497	25.210612630018197
39	24.696156179093748	22.26540561184373	27.21227506571114	25.826163143351383
40	23.39990564554175	21.694785792914427	29.10610383483477	25.799204726709053
41	22.930379889021186	22.716959090602746	28.710713724080605	25.641947296295463
42	23.927841304787364	21.445420438972885	28.18277806483499	26.44396019140476
43	22.087929368948398	22.233954125761013	29.317278098533013	26.360838406757576
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	2.0
4	3.0
5	3.0
6	3.0
7	3.0
8	5.0
9	7.0
10	9.0
11	11.0
12	11.0
13	20.0
14	29.0
15	53.0
16	77.0
17	76.5
18	76.0
19	76.0
20	94.0
21	112.0
22	121.0
23	130.0
24	172.0
25	214.0
26	214.0
27	245.5
28	277.0
29	493.0
30	709.0
31	766.0
32	823.0
33	823.0
34	942.5
35	1062.0
36	1138.5
37	1215.0
38	1700.5
39	2186.0
40	2186.0
41	2463.0
42	2740.0
43	2578.0
44	2416.0
45	2808.5
46	3201.0
47	3201.0
48	3621.5
49	4042.0
50	4535.0
51	5028.0
52	5328.0
53	5628.0
54	5628.0
55	4588.5
56	3549.0
57	3075.0
58	2601.0
59	2268.0
60	1935.0
61	1935.0
62	1819.5
63	1704.0
64	1539.0
65	1374.0
66	1185.5
67	997.0
68	997.0
69	877.0
70	757.0
71	651.0
72	545.0
73	460.0
74	375.0
75	375.0
76	295.5
77	216.0
78	193.0
79	170.0
80	144.5
81	119.0
82	119.0
83	108.5
84	98.0
85	72.5
86	47.0
87	33.0
88	19.0
89	19.0
90	14.5
91	10.0
92	7.0
93	4.0
94	3.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	44513.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.44955406285804
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.8261600537996	44.27021319614495
2	7.716879623402824	8.249275492552737
3	2.7824478816408877	4.461617954305484
4	1.550941492938803	3.315885247006493
5	0.8532279757901816	2.280232740997012
6	0.605245460659045	1.941005998247703
7	0.500168123739072	1.8713634219216857
8	0.4161062542030935	1.7792554983937277
9	0.30682582380632145	1.4759733111675242
>10	2.2948890383322125	24.008716554714354
>50	0.11348352387357094	4.1830476490014155
>100	0.03362474781439139	2.1634129355469187
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	153	0.3437198121896974	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	133	0.2987891177858154	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	126	0.28306337474445664	No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC	116	0.26059802754251565	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	111	0.2493653539415452	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	111	0.2493653539415452	No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC	108	0.24262574978096288	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	105	0.23588614562038054	No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC	94	0.21117426369824543	No Hit
GGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGATCTGG	87	0.19544852065688675	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	85	0.19095545121649854	No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT	85	0.19095545121649854	RNA PCR Primer, Index 17 (95% over 21bp)
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA	83	0.18646238177611035	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	81	0.18196931233572214	No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA	77	0.17298317345494577	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	76	0.17073663873475164	No Hit
ATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAGGGCAC	74	0.16624356929436343	No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT	72	0.16175049985397524	No Hit
GTCTTGAAGGGAACGTTGCCAGCTGCCTTGTACCAGCATTACA	72	0.16175049985397524	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	71	0.15950396513378115	No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA	70	0.15725743041358703	No Hit
CATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTCTTCACCTGCT	70	0.15725743041358703	No Hit
GTCCAGATACTTGCTGTAGTCACTGGTGAATGTGCCCTGTGAA	68	0.15276436097319884	No Hit
GGTGAATGTGCCCTGTGAATGGCGCTTGTCCTCGTTCATCTGA	66	0.14827129153281066	No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG	65	0.14602475681261656	No Hit
CTTCAGCATGTCTCTCAAATTCATCGTGACGTTTGGCAATGTT	64	0.14377822209242244	No Hit
GCCTGGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTT	60	0.13479208321164604	No Hit
ATTCTCAGCTTCCCAGGCAGACCCACTCAGTGATCCTGATCAG	58	0.13029901377125783	No Hit
TGCTGGTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGA	57	0.12805247905106373	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	57	0.12805247905106373	No Hit
GTGTTCATCTCATCAGAGAAAGAACCATCAGCATGTCTGCGGC	56	0.12580594433086964	No Hit
GCCTTGTACCAGCATTACAAATAATCCAGCCACAAAGTAAATG	56	0.12580594433086964	No Hit
ATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAGAT	55	0.12355940961067553	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	52	0.11681980545009323	No Hit
TTCCCAGGCAGACCCACTCAGTGATCCTGATCAGATGAACGAG	51	0.11457327072989913	No Hit
GTGAATGGCGCTTGTCCTCGTTCATCTGATCAGGATCACTGAG	50	0.11232673600970504	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	49	0.11008020128951093	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	49	0.11008020128951093	No Hit
CTGGTGAATGTGCCCTGTGAATGGCGCTTGTCCTCGTTCATCT	49	0.11008020128951093	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	49	0.11008020128951093	No Hit
GATCAGGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGA	49	0.11008020128951093	No Hit
GAGGAGAAATCCAGATCATTCTCAGCTTCCCAGGCAGACCCAC	48	0.10783366656931684	No Hit
TTGCTGTAGTCACTGGTGAATGTGCCCTGTGAATGGCGCTTGT	48	0.10783366656931684	No Hit
GGTGTATTCTGAGGCCACATTGCTTTGCATGCCAATAAATAAA	47	0.10558713184912272	No Hit
GTAAATGCTTTTCATTTCTGCTGTCTTCTGGTAGTGTGCTGTA	47	0.10558713184912272	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	46	0.10334059712892862	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	46	0.10334059712892862	No Hit
GGACTTAACATTTCAAACATCCCACGTGGCTAGCAGGTGATGT	46	0.10334059712892862	No Hit
ATCCAGATCATTCTCAGCTTCCCAGGCAGACCCACTCAGTGAT	46	0.10334059712892862	No Hit
CTTCTGGTAGTGTGCTGTAGAACAGAGCAGGTGAAGAGAGAGC	45	0.10109406240873453	No Hit
GAGTGGGTCTGCCTGGGAAGCTGAGAATGATCTGGATTTCTCC	45	0.10109406240873453	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0022465347201941007	0.0
26	0.0	0.0	0.0	0.004493069440388201	0.0
27	0.0	0.0	0.0	0.013479208321164603	0.0
28	0.0	0.0	0.0	0.06739604160582302	0.0
29	0.0	0.0	0.0	0.13029901377125783	0.0
30	0.0	0.0	0.0	0.23139307617999236	0.0
31	0.0	0.0	0.0	0.49423763844270213	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	20	0.0018229901	37.0	1
TTGAAGA	35	2.3374936E-5	31.714285	22
GTCGCCA	30	3.5446536E-4	30.833334	12
TGTTGAA	40	5.814752E-5	27.75	20
CATTGTT	40	5.814752E-5	27.75	17
AGAAGAG	40	5.814752E-5	27.75	5
ATTGTTG	40	5.814752E-5	27.75	18
GGACACA	35	8.7355723E-4	26.42857	6
CTTAGGA	35	8.7355723E-4	26.42857	2
TCGCCAT	35	8.7355723E-4	26.42857	13
TTAGGAC	35	8.7355723E-4	26.42857	3
AGCACAT	35	8.7355723E-4	26.42857	14
GCTTAGG	35	8.7355723E-4	26.42857	1
GAAGAGG	50	8.83748E-6	25.900002	6
GTTGAAG	45	1.2955071E-4	24.666664	21
CCATTGT	45	1.2955071E-4	24.666664	16
GCCATTG	45	1.2955071E-4	24.666664	15
TAGGACA	40	0.0019023241	23.125	4
AGAGGTC	40	0.0019023241	23.125	8
GAGCACA	40	0.0019023241	23.125	13
>>END_MODULE