FastQCFastQC Report
Fri 10 Feb 2017
ERR1632203.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632203.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772308
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG551.9016115E-523.5454545
GGTATCA4100.023.0121941
TATTAAC603.7242495E-521.5833322
GTATTAA705.098347E-621.1428571
TATGCCG450.003824689720.55555333
TCGTATG450.003824689720.55555330
TTTATAC701.2185402E-418.53
GTATATA801.6155514E-518.51
CGTATGC500.00703284518.49999831
CTCGTAT500.00703284518.49999829
TCAATAC500.00703284518.4999983
TCTTATA10400.017.43269237
CGTATAT650.001579299717.07692310
GTATGAA650.001579299717.0769231
GTATCAA5750.016.7304342
CTAGACA904.44292E-516.4444434
CTTATAC5550.015.66666737
CTCGAAC855.3613214E-415.2352943
TCGTTAA750.004102784714.828
CTCGTTA750.004102784714.827