FastQCFastQC Report
Fri 10 Feb 2017
ERR1632202.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632202.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2032500
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27870.13712177121771219No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22840.1123739237392374No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20540.10105781057810578No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATA358.8717486E-426.42857211
GGTATCA10350.020.3768121
TCGTATA650.001580498317.0769252
TAACACT2003.6379788E-1215.7254
CTTATAC13650.015.45054937
CAATACT1455.3516487E-815.3103464
TCGTTTA2500.014.79999930
GTATCAA14400.014.7743062
ATAACGA2900.014.67241512
TCTTATA22350.014.56823337
TCAATAC1551.211447E-714.3225813
ACGAACG2750.014.12727415
CTAACAC1458.918887E-714.0344843
GTCTTAA1652.5976988E-713.4545461
CGAACGA2900.013.39655316
GGGTATA1805.1644747E-813.3611121
TATCTAG4450.013.303371
TAACGAA3200.013.29687613
GTAACAC2102.042725E-913.2142853
TATATAC1551.8893497E-613.1290323
Produced by FastQC (version 0.11.5)