##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632202.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2032500
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08903862238623	33.0	31.0	34.0	30.0	34.0
2	32.278052152521525	34.0	31.0	34.0	30.0	34.0
3	32.370154489544895	34.0	31.0	34.0	30.0	34.0
4	35.99938942189422	37.0	35.0	37.0	35.0	37.0
5	35.61285116851168	37.0	35.0	37.0	33.0	37.0
6	35.58762066420664	37.0	35.0	37.0	33.0	37.0
7	35.56127773677737	37.0	35.0	37.0	33.0	37.0
8	35.531562115621156	37.0	35.0	37.0	33.0	37.0
9	37.22892496924969	39.0	37.0	39.0	34.0	39.0
10	37.07361377613776	39.0	37.0	39.0	33.0	39.0
11	37.17779040590406	39.0	37.0	39.0	34.0	39.0
12	37.11216088560886	39.0	37.0	39.0	33.0	39.0
13	37.17200295202952	39.0	37.0	39.0	34.0	39.0
14	38.36109372693727	40.0	38.0	41.0	34.0	41.0
15	38.32423960639606	40.0	38.0	41.0	34.0	41.0
16	38.31048757687577	40.0	38.0	41.0	34.0	41.0
17	38.27502779827798	40.0	38.0	41.0	34.0	41.0
18	38.25783271832719	40.0	38.0	41.0	34.0	41.0
19	38.27834784747847	40.0	38.0	41.0	34.0	41.0
20	38.24682115621156	40.0	38.0	41.0	34.0	41.0
21	38.212762607626075	40.0	38.0	41.0	34.0	41.0
22	38.16173874538745	40.0	38.0	41.0	34.0	41.0
23	38.10083837638376	40.0	38.0	41.0	34.0	41.0
24	38.02303960639606	40.0	37.0	41.0	33.0	41.0
25	37.94713849938499	40.0	37.0	41.0	33.0	41.0
26	37.818062484624846	39.0	37.0	41.0	33.0	41.0
27	37.71718671586716	39.0	37.0	41.0	33.0	41.0
28	37.617651168511685	39.0	37.0	40.0	33.0	41.0
29	37.52377613776138	39.0	37.0	40.0	32.0	41.0
30	37.42626617466175	39.0	37.0	40.0	32.0	41.0
31	37.33106617466175	39.0	36.0	40.0	32.0	41.0
32	37.223861746617466	39.0	36.0	40.0	31.0	41.0
33	37.11658007380074	39.0	36.0	40.0	31.0	41.0
34	37.02171266912669	39.0	36.0	40.0	31.0	41.0
35	36.97632177121771	39.0	36.0	40.0	31.0	41.0
36	36.91718671586716	39.0	36.0	40.0	31.0	41.0
37	36.85248118081181	39.0	35.0	40.0	31.0	41.0
38	36.78128708487085	39.0	35.0	40.0	31.0	41.0
39	36.71810135301353	39.0	35.0	40.0	31.0	41.0
40	36.62625043050431	39.0	35.0	40.0	30.0	41.0
41	36.556632226322265	38.0	35.0	40.0	30.0	41.0
42	36.47692054120541	38.0	35.0	40.0	30.0	41.0
43	35.48309175891759	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	1.0
13	5.0
14	4.0
15	9.0
16	27.0
17	48.0
18	94.0
19	214.0
20	395.0
21	759.0
22	1237.0
23	2041.0
24	3277.0
25	5092.0
26	7654.0
27	10883.0
28	15600.0
29	21181.0
30	28555.0
31	38885.0
32	51937.0
33	70733.0
34	100474.0
35	146679.0
36	231312.0
37	382792.0
38	582714.0
39	329896.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.32910209102091	17.730774907749076	11.718425584255844	27.221697416974173
2	20.190848708487085	20.030110701107013	32.07439114391144	27.704649446494468
3	20.43020910209102	21.195571955719558	28.83822878228782	29.535990159901598
4	15.487576875768758	15.084182041820418	33.1550307503075	36.273210332103325
5	16.812103321033213	33.903222632226324	32.60383763837638	16.680836408364083
6	36.457023370233706	33.53722017220172	14.983075030750307	15.02268142681427
7	31.30587945879459	28.83227552275523	20.101156211562117	19.76068880688807
8	28.889643296432965	31.20551045510455	19.154292742927428	20.750553505535056
9	27.710848708487084	13.497761377613775	18.35109471094711	40.44029520295203
10	18.083345633456336	24.90391143911439	31.559261992619923	25.45348093480935
11	38.61554735547356	20.006642066420664	20.334464944649447	21.043345633456333
12	23.504452644526445	23.515276752767527	27.699483394833948	25.28078720787208
13	31.773087330873306	17.777170971709715	23.015153751537515	27.434587945879457
14	24.063517835178352	18.86735547355474	23.396851168511684	33.67227552275523
15	26.924280442804427	25.707699876998767	20.651857318573185	26.716162361623617
16	27.046838868388683	24.244034440344404	22.267257072570725	26.441869618696185
17	25.82661746617466	24.267158671586717	23.636162361623615	26.270061500615004
18	26.16782287822878	23.14214022140221	24.484772447724477	26.205264452644528
19	26.56359163591636	23.513456334563347	24.146863468634685	25.776088560885608
20	26.73230012300123	23.094366543665437	23.8240098400984	26.349323493234934
21	27.30573185731857	23.442952029520296	23.41239852398524	25.838917589175892
22	27.162558425584255	23.213431734317343	23.409446494464945	26.214563345633458
23	26.47537515375154	23.00619926199262	23.633505535055352	26.884920049200495
24	26.526346863468635	23.525116851168512	23.50479704797048	26.44373923739237
25	26.6820172201722	22.808019680196804	23.720442804428043	26.78952029520295
26	26.347257072570724	23.592619926199262	23.793062730627305	26.267060270602705
27	26.551439114391144	23.25279212792128	23.528659286592866	26.66710947109471
28	26.23581795817958	23.314440344403444	24.05333333333333	26.39640836408364
29	25.908487084870846	23.531758917589176	24.216039360393605	26.34371463714637
30	25.62789667896679	23.898499384993848	23.971955719557194	26.501648216482167
31	26.237539975399756	23.55837638376384	23.53161131611316	26.672472324723245
32	25.874440344403443	23.519261992619928	23.72516605166052	26.88113161131611
33	25.69510455104551	23.17741697416974	24.419089790897907	26.70838868388684
34	26.493333333333336	22.843936039360393	24.21643296432964	26.44629766297663
35	26.230504305043052	22.906371463714635	24.714587945879458	26.148536285362855
36	25.559261992619923	23.41490774907749	24.412201722017222	26.61362853628536
37	25.794391143911437	22.58858548585486	24.826174661746617	26.790848708487086
38	25.160098400984012	22.474489544895448	25.738745387453875	26.626666666666665
39	25.087576875768757	22.21918819188192	25.8630258302583	26.83020910209102
40	24.871488314883152	22.169643296432966	26.465436654366542	26.49343173431734
41	24.21741697416974	22.25771217712177	27.05810578105781	26.466765067650677
42	24.6659778597786	22.327183271832716	27.039409594095943	25.96742927429274
43	23.798769987699878	21.638573185731858	27.415301353013533	27.147355473554736
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	104.0
1	119.5
2	135.0
3	294.5
4	454.0
5	454.0
6	592.0
7	730.0
8	715.5
9	701.0
10	967.5
11	1234.0
12	1234.0
13	2016.0
14	2798.0
15	4361.5
16	5925.0
17	5828.0
18	5731.0
19	5731.0
20	6906.5
21	8082.0
22	8233.0
23	8384.0
24	10449.5
25	12515.0
26	12515.0
27	15359.0
28	18203.0
29	22366.5
30	26530.0
31	31692.0
32	36854.0
33	36854.0
34	44504.0
35	52154.0
36	60714.0
37	69274.0
38	76834.0
39	84394.0
40	84394.0
41	91304.5
42	98215.0
43	104477.0
44	110739.0
45	118360.0
46	125981.0
47	125981.0
48	131784.0
49	137587.0
50	141664.0
51	145741.0
52	149516.5
53	153292.0
54	153292.0
55	152359.0
56	151426.0
57	150365.5
58	149305.0
59	146610.5
60	143916.0
61	143916.0
62	134507.5
63	125099.0
64	113921.5
65	102744.0
66	91826.0
67	80908.0
68	80908.0
69	69973.5
70	59039.0
71	51395.5
72	43752.0
73	35325.5
74	26899.0
75	26899.0
76	21713.0
77	16527.0
78	13543.5
79	10560.0
80	9035.0
81	7510.0
82	7510.0
83	5766.5
84	4023.0
85	3286.5
86	2550.0
87	2027.5
88	1505.0
89	1505.0
90	1159.0
91	813.0
92	466.5
93	120.0
94	79.0
95	38.0
96	38.0
97	23.0
98	8.0
99	4.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2032500.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.593405027782254
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.48958260232966	53.51084069483667
2	8.238799763116171	10.31389061031054
3	2.411589919003632	4.528488736833328
4	1.1587293065104682	2.9011525119988395
5	0.6268558659244762	1.9618521554925952
6	0.4284200074001564	1.6089760227122072
7	0.28853022367740266	1.2642062407377392
8	0.20195202359890185	1.0112691847445046
9	0.1454333528153241	0.8192851881586145
>10	0.8859151212218235	10.163553344670412
>50	0.06744805116212854	2.9109681747056713
>100	0.05138636432093643	6.378687613349728
>500	0.004727116693114365	1.9903287116917936
>1k	6.302822257485784E-4	0.6365008097573306
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2787	0.13712177121771219	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2284	0.1123739237392374	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2054	0.10105781057810578	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.4760147601476016E-4	0.0
10	0.0	0.0	0.0	1.4760147601476016E-4	0.0
11	0.0	0.0	0.0	1.968019680196802E-4	0.0
12	0.0	0.0	0.0	1.968019680196802E-4	0.0
13	0.0	0.0	0.0	1.968019680196802E-4	0.0
14	0.0	0.0	0.0	1.968019680196802E-4	0.0
15	0.0	0.0	0.0	1.968019680196802E-4	0.0
16	0.0	0.0	0.0	5.412054120541205E-4	0.0
17	0.0	0.0	0.0	9.840098400984009E-4	0.0
18	0.0	0.0	0.0	0.0012300123001230013	0.0
19	0.0	0.0	0.0	0.0013776137761377615	0.0
20	0.0	0.0	0.0	0.0015252152521525214	0.0
21	0.0	0.0	0.0	0.0016728167281672816	0.0
22	0.0	0.0	0.0	0.0024108241082410825	0.0
23	0.0	0.0	0.0	0.002952029520295203	0.0
24	0.0	0.0	0.0	0.004772447724477245	0.0
25	0.0	0.0	0.0	0.0064944649446494465	0.0
26	0.0	0.0	0.0	0.009249692496924969	0.0
27	0.0	0.0	0.0	0.025731857318573186	0.0
28	0.0	4.920049200492005E-5	0.0	0.0822140221402214	0.0
29	0.0	4.920049200492005E-5	0.0	0.16339483394833948	0.0
30	0.0	4.920049200492005E-5	0.0	0.2830012300123001	0.0
31	0.0	4.920049200492005E-5	0.0	0.5691512915129151	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCATA	35	8.8717486E-4	26.428572	11
GGTATCA	1035	0.0	20.376812	1
TCGTATA	65	0.0015804983	17.076925	2
TAACACT	200	3.6379788E-12	15.725	4
CTTATAC	1365	0.0	15.450549	37
CAATACT	145	5.3516487E-8	15.310346	4
TCGTTTA	250	0.0	14.799999	30
GTATCAA	1440	0.0	14.774306	2
ATAACGA	290	0.0	14.672415	12
TCTTATA	2235	0.0	14.568233	37
TCAATAC	155	1.211447E-7	14.322581	3
ACGAACG	275	0.0	14.127274	15
CTAACAC	145	8.918887E-7	14.034484	3
GTCTTAA	165	2.5976988E-7	13.454546	1
CGAACGA	290	0.0	13.396553	16
GGGTATA	180	5.1644747E-8	13.361112	1
TATCTAG	445	0.0	13.30337	1
TAACGAA	320	0.0	13.296876	13
GTAACAC	210	2.042725E-9	13.214285	3
TATATAC	155	1.8893497E-6	13.129032	3
>>END_MODULE