FastQCFastQC Report
Fri 10 Feb 2017
ERR1632201.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632201.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1739844
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7050.024.6666661
TCCGTAT502.7028765E-422.19999913
CTTATAC16600.018.38855237
CTAGAAC4300.018.0697673
GTTCTAG3400.017.9558811
TCTAGAC3400.017.9558813
CTAGACA3650.017.7397254
CCTAGTA1054.800768E-717.619052
TAGAACT5050.017.584164
GTATCAA11900.017.5672281
CGTTATT1352.2213499E-816.4444432
GGACCGT803.3838145E-416.18756
ACTGATC3550.016.154938
TCTTATA29450.016.14431237
TTTCGGA1504.6748028E-916.03333330
CAACGGA2900.015.94827624
ATCCGTA700.00259321115.85714312
AACGGAG3050.015.77049325
GTCTAGA4000.015.7249991
ACGGAGT3200.015.60937626