##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632201.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1739844
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70364067123259	34.0	31.0	34.0	31.0	34.0
2	32.8818928593598	34.0	31.0	34.0	31.0	34.0
3	32.97036228535432	34.0	31.0	34.0	31.0	34.0
4	36.39177707886454	37.0	37.0	37.0	35.0	37.0
5	36.24830731950681	37.0	37.0	37.0	35.0	37.0
6	36.2700983536455	37.0	37.0	37.0	35.0	37.0
7	36.249830444568595	37.0	37.0	37.0	35.0	37.0
8	36.230474686236235	37.0	36.0	37.0	35.0	37.0
9	38.0146835003598	39.0	38.0	39.0	35.0	39.0
10	37.976025436763294	39.0	38.0	39.0	35.0	39.0
11	38.048392269651764	39.0	38.0	39.0	35.0	39.0
12	38.0167779410108	39.0	38.0	39.0	35.0	39.0
13	38.03229599895163	39.0	38.0	39.0	35.0	39.0
14	39.42187288055711	40.0	39.0	41.0	37.0	41.0
15	39.42268559709951	40.0	39.0	41.0	37.0	41.0
16	39.43327505224606	40.0	39.0	41.0	37.0	41.0
17	39.420393437572564	40.0	39.0	41.0	37.0	41.0
18	39.420628516119834	40.0	39.0	41.0	37.0	41.0
19	39.4373380601939	40.0	39.0	41.0	37.0	41.0
20	39.416882777996186	40.0	39.0	41.0	37.0	41.0
21	39.39292085957132	40.0	39.0	41.0	37.0	41.0
22	39.3639113621681	40.0	39.0	41.0	37.0	41.0
23	39.32174838663696	40.0	39.0	41.0	36.0	41.0
24	39.28309664544637	40.0	39.0	41.0	36.0	41.0
25	39.2336841693853	40.0	39.0	41.0	36.0	41.0
26	39.16209671671713	40.0	39.0	41.0	36.0	41.0
27	39.089772991141736	40.0	39.0	41.0	36.0	41.0
28	39.0353870806808	40.0	39.0	41.0	36.0	41.0
29	38.98694940465927	40.0	39.0	41.0	36.0	41.0
30	38.92190046923747	40.0	38.0	41.0	35.0	41.0
31	38.86384124093884	40.0	38.0	41.0	35.0	41.0
32	38.78720218594311	40.0	38.0	41.0	35.0	41.0
33	38.72191932150239	40.0	38.0	41.0	35.0	41.0
34	38.659449352930494	40.0	38.0	41.0	35.0	41.0
35	38.649102448265474	40.0	38.0	41.0	35.0	41.0
36	38.61503445136461	40.0	38.0	41.0	35.0	41.0
37	38.57163343380211	40.0	38.0	41.0	35.0	41.0
38	38.53302479992459	40.0	38.0	41.0	35.0	41.0
39	38.490777334059835	40.0	38.0	41.0	35.0	41.0
40	38.43912787583255	40.0	38.0	41.0	34.0	41.0
41	38.40214065169061	40.0	38.0	41.0	34.0	41.0
42	38.3585235227986	40.0	38.0	41.0	34.0	41.0
43	37.50419003083035	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	6.0
16	2.0
17	8.0
18	21.0
19	41.0
20	74.0
21	128.0
22	288.0
23	487.0
24	820.0
25	1285.0
26	1996.0
27	3062.0
28	4709.0
29	6658.0
30	10018.0
31	13903.0
32	19884.0
33	27817.0
34	41455.0
35	63930.0
36	104248.0
37	194405.0
38	479745.0
39	764853.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.39929326997133	16.908355001942706	11.644492264823743	28.047859463262228
2	19.610953625727365	19.559397279296302	33.02934056156759	27.800308533408742
3	20.284634714376693	20.4194169132405	28.8156294472378	30.48031892514501
4	15.603123038617255	14.641657527916296	32.411009262899434	37.34421017056702
5	16.85628136775481	34.040235791254844	32.64143222035998	16.462050620630357
6	37.37404043121108	32.59280717121765	14.797131237053437	15.23602116051784
7	32.28818215886022	28.331275677589485	19.536004377403952	19.844537786146343
8	29.098126038886246	31.533574274475185	19.04073008844471	20.32756959819386
9	28.357255018266002	13.348495612250295	17.905973179204572	40.38827619027913
10	17.76331671115341	25.447453909660865	31.43000177027366	25.359227608912065
11	38.923087357257316	19.746540494435134	19.875057763799514	21.455314384508036
12	24.14544062571127	23.27720186407517	27.37894891725925	25.198408592954312
13	32.68258533523695	17.78383579217447	22.492591289793797	27.040987582794777
14	24.69537498764257	18.22939298006028	22.966139492966036	34.109092539331115
15	27.051275861514018	25.58373049537775	20.332110235170507	27.032883407937724
16	27.403893682422105	23.82317035320408	21.71033724862689	27.062598715746926
17	26.151022735371676	24.403912074875677	23.100634309742713	26.344430880009934
18	26.54174742103315	22.92734291120353	23.924041465786587	26.60686820197673
19	27.117431217971266	23.260763608691352	23.47561045702948	26.146194716307896
20	27.172148767360753	23.104312800457972	23.555675106503802	26.167863325677477
21	27.6834015003644	23.02384581606167	23.174319076882753	26.118433606691177
22	27.967047620361367	22.99459031959187	22.47546331740087	26.562898742645892
23	26.786999294189595	23.021086948025225	22.94556293552755	27.246350822257625
24	26.734753230749426	23.19713721460085	22.95958718137948	27.10852237327025
25	27.09294626414782	22.681861132377385	23.20558624796246	27.019606355512334
26	26.99132795813878	22.687608774119976	23.12569402774042	27.195369240000826
27	26.967762626994148	22.92015835902529	23.40232802481142	26.709750989169144
28	26.456394941155644	23.023903292479094	23.371693094323398	27.14800867204186
29	26.17907122707553	23.23627865486791	23.44721710682107	27.13743301123549
30	25.795301187922597	23.516763571906445	23.936111513446033	26.751823726724925
31	26.678541294506864	23.201562898742644	22.958552605865815	27.161343200884673
32	26.069406222626856	23.088564262083267	23.744082802826	27.097946712463877
33	25.997848082931576	22.805148047756006	23.925650805474515	27.271353063837907
34	26.677909053915176	22.11014320824166	24.057674136301877	27.15427360154129
35	25.925945084731733	22.330392839817822	24.820558624796245	26.923103450654196
36	25.789208687675448	22.061863017603876	24.864240702039954	27.284687592680722
37	25.834500104607077	21.499513749508576	25.327558102910373	27.338428042973966
38	25.095985617101302	21.223282087359557	26.414724538521845	27.266007757017295
39	25.08684686673058	20.901586579026628	26.930345479249862	27.08122107499293
40	24.60806830957258	21.099133025719546	27.319345872388556	26.973452792319314
41	24.084745528909487	20.52701276666184	28.33305744652969	27.055184257898983
42	23.402155595559144	20.77289688040997	28.597334013854116	27.227613510176774
43	22.487935699982298	20.509999747103763	28.929777612245694	28.072286940668246
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	33.0
1	33.5
2	34.0
3	75.5
4	117.0
5	117.0
6	165.0
7	213.0
8	209.0
9	205.0
10	299.5
11	394.0
12	394.0
13	648.5
14	903.0
15	1433.0
16	1963.0
17	2081.5
18	2200.0
19	2200.0
20	2868.0
21	3536.0
22	4034.0
23	4532.0
24	5827.5
25	7123.0
26	7123.0
27	8823.5
28	10524.0
29	13556.0
30	16588.0
31	20506.5
32	24425.0
33	24425.0
34	30192.0
35	35959.0
36	42316.0
37	48673.0
38	54637.0
39	60601.0
40	60601.0
41	68226.0
42	75851.0
43	84290.0
44	92729.0
45	102881.0
46	113033.0
47	113033.0
48	119851.0
49	126669.0
50	133693.0
51	140717.0
52	146834.5
53	152952.0
54	152952.0
55	152147.0
56	151342.0
57	147549.5
58	143757.0
59	140746.0
60	137735.0
61	137735.0
62	129200.5
63	120666.0
64	105218.5
65	89771.0
66	78962.0
67	68153.0
68	68153.0
69	55546.5
70	42940.0
71	36016.0
72	29092.0
73	22213.0
74	15334.0
75	15334.0
76	12034.0
77	8734.0
78	7008.5
79	5283.0
80	4448.0
81	3613.0
82	3613.0
83	2639.5
84	1666.0
85	1336.5
86	1007.0
87	749.5
88	492.0
89	492.0
90	357.5
91	223.0
92	130.0
93	37.0
94	27.0
95	17.0
96	17.0
97	11.5
98	6.0
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1739844.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.08571239805442
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.40748299019454	44.27745653863083
2	8.385891554129218	8.903420544875546
3	2.77242743811057	4.415288568720375
4	1.362134669560164	2.89239557262759
5	0.809312449148673	2.148146395783575
6	0.5471579250052798	1.7427760945887907
7	0.4015142161250442	1.492026774066105
8	0.31000537350453633	1.3165484879770604
9	0.23370954925348475	1.116597412471448
>10	1.5081491483320533	15.401772800510113
>50	0.15854146869842428	5.8067142242573615
>100	0.10085674594561547	9.469238426051426
>500	0.0028164719922924293	1.0176181594398304
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.2990566970372057E-4	0.0
10	0.0	5.747641742593014E-5	0.0	2.873820871296507E-4	5.747641742593014E-5
11	0.0	5.747641742593014E-5	0.0	4.02334921981511E-4	5.747641742593014E-5
12	0.0	5.747641742593014E-5	0.0	4.5981133940744114E-4	5.747641742593014E-5
13	0.0	5.747641742593014E-5	0.0	5.747641742593014E-4	5.747641742593014E-5
14	0.0	5.747641742593014E-5	0.0	6.897170091111617E-4	5.747641742593014E-5
15	0.0	5.747641742593014E-5	0.0	8.04669843963022E-4	1.1495283485186028E-4
16	0.0	5.747641742593014E-5	0.0	0.0013219576007963932	1.1495283485186028E-4
17	0.0	5.747641742593014E-5	0.0	0.0017817689402038345	1.1495283485186028E-4
18	0.0	5.747641742593014E-5	0.0	0.0018967217750556946	1.1495283485186028E-4
19	0.0	5.747641742593014E-5	0.0	0.0021841038621853454	1.1495283485186028E-4
20	0.0	5.747641742593014E-5	0.0	0.0023565331144631356	1.1495283485186028E-4
21	0.0	5.747641742593014E-5	0.0	0.0027013916190187165	1.1495283485186028E-4
22	0.0	5.747641742593014E-5	0.0	0.0035060614629817384	1.1495283485186028E-4
23	0.0	5.747641742593014E-5	0.0	0.00431073130694476	1.1495283485186028E-4
24	0.0	5.747641742593014E-5	0.0	0.006035023829722665	1.1495283485186028E-4
25	0.0	5.747641742593014E-5	0.0	0.007529410682796849	1.1495283485186028E-4
26	0.0	5.747641742593014E-5	0.0	0.010690613641223007	1.1495283485186028E-4
27	0.0	5.747641742593014E-5	0.0	0.03230174659337274	1.1495283485186028E-4
28	0.0	5.747641742593014E-5	0.0	0.11248134890254528	1.1495283485186028E-4
29	0.0	5.747641742593014E-5	0.0	0.2364579812902766	1.1495283485186028E-4
30	0.0	5.747641742593014E-5	0.0	0.3943456999593067	1.1495283485186028E-4
31	0.0	5.747641742593014E-5	0.0	0.8692158607323415	1.1495283485186028E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	705	0.0	24.666666	1
TCCGTAT	50	2.7028765E-4	22.199999	13
CTTATAC	1660	0.0	18.388552	37
CTAGAAC	430	0.0	18.069767	3
GTTCTAG	340	0.0	17.955881	1
TCTAGAC	340	0.0	17.955881	3
CTAGACA	365	0.0	17.739725	4
CCTAGTA	105	4.800768E-7	17.61905	2
TAGAACT	505	0.0	17.58416	4
GTATCAA	1190	0.0	17.567228	1
CGTTATT	135	2.2213499E-8	16.444443	2
GGACCGT	80	3.3838145E-4	16.1875	6
ACTGATC	355	0.0	16.15493	8
TCTTATA	2945	0.0	16.144312	37
TTTCGGA	150	4.6748028E-9	16.033333	30
CAACGGA	290	0.0	15.948276	24
ATCCGTA	70	0.002593211	15.857143	12
AACGGAG	305	0.0	15.770493	25
GTCTAGA	400	0.0	15.724999	1
ACGGAGT	320	0.0	15.609376	26
>>END_MODULE