FastQCFastQC Report
Fri 10 Feb 2017
ERR1632200.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632200.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328491
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG6390.1945258774212992No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG5800.17656495916174264No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC5660.17230304635439023No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC5620.17108535698086097No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG5180.15769077387203911No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT5140.15647308449850986No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA4890.148862525913952No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC4600.14003427795586484No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC4400.13394583108821853No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG4360.1327281417146893No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA4350.13242371937130698No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA4300.1309016076543954No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG4170.1269441171904253No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT4010.12207335969630828No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT3820.11628933517204429No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC3800.11568049048527966No Hit
GTCCCTGGCTGCTGCCGCGGGGAAGCAGGAACTGGCCAAGTAC3790.11537606814189735No Hit
GAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGGCGCCGA3770.11476722345513271No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA3750.1141583787683681No Hit
CGGCAGGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAG3580.10898319893086873No Hit
ATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCA3570.1086787765874864No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC3550.10806993190072178No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA3490.1062433978404279No Hit
GTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCATCGTC3430.104416863780134No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG3410.10380801909336938No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG3390.10319917440660474No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA3300.1004593733161639No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG3290.1001549509727816No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACC250.005490699329.629
TCATACA250.005490699329.62
TTTCGTC405.9274156E-527.75000217
GGTATCA1700.027.2058831
TGTATTG358.855858E-426.428575
ATTGTTA451.3204027E-424.6666688
CTACTAG400.001928267423.1250021
TTAATAC502.6964117E-422.23
CTAATTT502.6964117E-422.229
TTCGTCA450.00382007920.55555718
TTTTTCG450.00382007920.55555715
CTTGCGC450.00382007920.5555573
TCACTAC450.00382007920.55555722
TTGTTAT450.00382007920.5555579
TGTACCG450.00382007920.5555575
GGCTAAT450.00382007920.55555727
CCTGTAT656.882966E-519.9230773
TCCTGTA759.23277E-619.7333342
AGTCAGA500.007024448418.56
GACCCGC500.007024448418.511
Produced by FastQC (version 0.11.5)