##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632200.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	328491
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.971353857487724	33.0	31.0	34.0	30.0	34.0
2	32.163216039404425	33.0	31.0	34.0	30.0	34.0
3	32.26295697599021	34.0	31.0	34.0	30.0	34.0
4	35.90829581327951	37.0	35.0	37.0	35.0	37.0
5	35.52362774018162	37.0	35.0	37.0	33.0	37.0
6	35.50384028786177	37.0	35.0	37.0	33.0	37.0
7	35.4783266512629	37.0	35.0	37.0	33.0	37.0
8	35.44750693321887	37.0	35.0	37.0	33.0	37.0
9	37.161094215671056	39.0	37.0	39.0	34.0	39.0
10	36.98742126877144	39.0	37.0	39.0	33.0	39.0
11	37.113199448386716	39.0	37.0	39.0	33.0	39.0
12	37.01810704098438	39.0	37.0	39.0	33.0	39.0
13	37.085968869771165	39.0	37.0	39.0	33.0	39.0
14	38.26404071953265	40.0	38.0	41.0	34.0	41.0
15	38.219780755028296	40.0	38.0	41.0	34.0	41.0
16	38.22309591434773	40.0	38.0	41.0	34.0	41.0
17	38.15854315643351	40.0	38.0	41.0	33.0	41.0
18	38.142006934740984	40.0	38.0	41.0	33.0	41.0
19	38.13747104182458	40.0	38.0	41.0	34.0	41.0
20	38.12330627018701	40.0	38.0	41.0	34.0	41.0
21	38.081776974102794	40.0	38.0	41.0	34.0	41.0
22	38.052129282080784	40.0	37.0	41.0	33.0	41.0
23	38.00063624269767	40.0	37.0	41.0	33.0	41.0
24	37.924853344536075	40.0	37.0	41.0	33.0	41.0
25	37.85485751512218	39.0	37.0	41.0	33.0	41.0
26	37.71903035395186	39.0	37.0	41.0	33.0	41.0
27	37.627441847721855	39.0	37.0	40.0	33.0	41.0
28	37.53275432203622	39.0	37.0	40.0	32.0	41.0
29	37.42837703316073	39.0	37.0	40.0	32.0	41.0
30	37.3453732370141	39.0	37.0	40.0	32.0	41.0
31	37.21120822183865	39.0	36.0	40.0	31.0	41.0
32	37.104218989256935	39.0	36.0	40.0	31.0	41.0
33	37.01791829913148	39.0	36.0	40.0	31.0	41.0
34	36.90791224112685	39.0	36.0	40.0	31.0	41.0
35	36.88326011975975	39.0	36.0	40.0	31.0	41.0
36	36.833356165009086	39.0	36.0	40.0	31.0	41.0
37	36.750991655783565	39.0	35.0	40.0	31.0	41.0
38	36.74090614354731	39.0	35.0	40.0	31.0	41.0
39	36.653448648517006	39.0	35.0	40.0	30.0	41.0
40	36.57172342621259	38.0	35.0	40.0	30.0	41.0
41	36.50647049690859	38.0	35.0	40.0	30.0	41.0
42	36.465315640306734	38.0	35.0	40.0	30.0	41.0
43	35.48449424793982	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.0
17	7.0
18	7.0
19	31.0
20	50.0
21	100.0
22	210.0
23	321.0
24	535.0
25	875.0
26	1260.0
27	1814.0
28	2614.0
29	3714.0
30	5003.0
31	6665.0
32	9094.0
33	12161.0
34	17300.0
35	25020.0
36	38455.0
37	61306.0
38	90730.0
39	51212.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.05752364600552	17.621182924341916	11.707778904140447	26.613514525512116
2	20.260829063809968	19.734482832101946	32.803029611161335	27.201658492926743
3	21.68400351912229	19.928095442493095	27.720394166050212	30.6675068723344
4	15.51092724001571	15.156579632318692	32.076069055164375	37.256424072501225
5	17.725599788122047	33.07883625426572	32.121427984328335	17.074135973283894
6	38.53134484658636	31.59051541746958	14.76143943060845	15.116700305335609
7	33.038956927282634	27.233318416638507	19.10402415895717	20.623700497121686
8	29.124085591386066	31.036771174857147	19.136597349699077	20.702545884057706
9	28.01355288272738	12.563510111388135	17.818144180510274	41.60479282537421
10	18.030630976191127	24.880742546979977	31.593255218560024	25.49537125826887
11	39.27717958787303	19.5889689519652	19.048314870118208	22.085536590043564
12	23.59212276744264	22.94674739947213	26.773640678131212	26.68748915495402
13	33.52968574481493	17.303974842537542	22.091625036911207	27.07471437573632
14	25.425962963977707	18.607815739243996	22.324812551942063	33.64140874483624
15	26.87349120676061	25.21408501298361	20.489145821346703	27.423277958909072
16	28.84888779296845	22.787838936226564	21.758586993250955	26.60468627755403
17	26.651871740778287	23.497142996307357	22.577787519292766	27.27319774362159
18	26.35505995598053	22.06148722491636	23.958342846531565	27.62510997257155
19	28.479927912789087	23.47157151946324	23.078866696500057	24.969633871247616
20	28.304276220657492	22.55617353291262	22.67581151386187	26.463738732568014
21	27.546264585635527	23.18237029325004	22.055703200392095	27.215661920722333
22	29.012058169021373	22.176254448371495	22.032871524638423	26.778815857968713
23	26.05215972431513	22.810061767293472	22.68372649478981	28.45405201360159
24	27.051273855295882	23.08312860930741	22.526035720917772	27.339561814478934
25	27.220532678216454	22.42557634760161	22.803668898082442	27.550222076099494
26	27.506385258652443	22.809452922606706	21.69557156817082	27.988590250570034
27	27.790411305028144	22.613404933468498	21.932107728978874	27.66407603252448
28	27.029355446572357	23.204897546660337	22.544909906207476	27.22083710055983
29	27.42145142484878	23.423168366865454	23.143404233297108	26.011975974988662
30	27.67807946032007	22.399091603727346	21.945502312087697	27.977326623864883
31	26.149270451854083	22.509596914375127	22.841721691005233	28.499410942765557
32	25.670109683370317	23.105960285061084	23.27552353032503	27.948406501243568
33	26.534669138576096	21.888575333875202	23.741898560386737	27.834856967161965
34	27.47198553385024	21.95250402598549	24.113598241656543	26.46191219850772
35	27.5054719916223	21.546404619913485	23.30687903169341	27.641244356770812
36	25.660368168382085	21.646863993229648	23.554678819206615	29.138089019181653
37	24.846647244521158	21.120517761521622	26.202848784289372	27.829986209667844
38	24.81894481127337	20.49279888946729	25.63570995856812	29.052546340691222
39	24.290163200818288	20.898593873195917	27.987676983539885	26.82356594244591
40	25.460667111123286	19.981064930241622	27.293594040628204	27.26467391800689
41	23.367763500369872	20.648358706935653	28.448267989077326	27.535609803617145
42	22.578091941636146	21.325698420961306	28.68236877113833	27.413840866264223
43	22.489200617368514	19.90374165502251	28.45831392640894	29.148743801200034
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	10.0
2	6.0
3	9.5
4	13.0
5	13.0
6	22.0
7	31.0
8	36.5
9	42.0
10	57.5
11	73.0
12	73.0
13	114.5
14	156.0
15	246.0
16	336.0
17	371.5
18	407.0
19	407.0
20	548.0
21	689.0
22	836.0
23	983.0
24	1238.0
25	1493.0
26	1493.0
27	1847.0
28	2201.0
29	2750.5
30	3300.0
31	3974.5
32	4649.0
33	4649.0
34	6225.5
35	7802.0
36	8759.5
37	9717.0
38	10608.5
39	11500.0
40	11500.0
41	12400.5
42	13301.0
43	15106.0
44	16911.0
45	17349.5
46	17788.0
47	17788.0
48	18688.5
49	19589.0
50	21448.0
51	23307.0
52	25929.0
53	28551.0
54	28551.0
55	28622.0
56	28693.0
57	26893.0
58	25093.0
59	25991.5
60	26890.0
61	26890.0
62	24929.0
63	22968.0
64	19332.5
65	15697.0
66	14624.0
67	13551.0
68	13551.0
69	12089.5
70	10628.0
71	10719.5
72	10811.0
73	8868.5
74	6926.0
75	6926.0
76	4405.0
77	1884.0
78	1465.5
79	1047.0
80	868.5
81	690.0
82	690.0
83	534.5
84	379.0
85	293.0
86	207.0
87	149.5
88	92.0
89	92.0
90	78.0
91	64.0
92	36.5
93	9.0
94	6.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	328491.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.87401301603862
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.31079206587788	61.731931101343505
2	4.263103139545287	5.701816296853333
3	1.109987032341294	2.2268786174527726
4	0.5483713732967126	1.466871774218693
5	0.3211525885325204	1.073838119282913
6	0.21559415688115272	0.8650587872071246
7	0.16297768481955455	0.7629280271162712
8	0.12603611070714585	0.6742832406336444
9	0.09556153959862007	0.5751525278655727
>10	0.6798062362057529	9.019635688585549
>50	0.08125484068643962	3.8729827804040267
>100	0.08262430429351203	10.997109772308006
>500	0.0027389272141495704	1.0315132667285352
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	639	0.1945258774212992	No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG	580	0.17656495916174264	No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC	566	0.17230304635439023	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	562	0.17108535698086097	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	518	0.15769077387203911	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	514	0.15647308449850986	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	489	0.148862525913952	No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC	460	0.14003427795586484	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	440	0.13394583108821853	No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG	436	0.1327281417146893	No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA	435	0.13242371937130698	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	430	0.1309016076543954	No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG	417	0.1269441171904253	No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT	401	0.12207335969630828	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	382	0.11628933517204429	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	380	0.11568049048527966	No Hit
GTCCCTGGCTGCTGCCGCGGGGAAGCAGGAACTGGCCAAGTAC	379	0.11537606814189735	No Hit
GAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGGCGCCGA	377	0.11476722345513271	No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA	375	0.1141583787683681	No Hit
CGGCAGGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAG	358	0.10898319893086873	No Hit
ATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCA	357	0.1086787765874864	No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC	355	0.10806993190072178	No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA	349	0.1062433978404279	No Hit
GTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCATCGTC	343	0.104416863780134	No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG	341	0.10380801909336938	No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG	339	0.10319917440660474	No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA	330	0.1004593733161639	No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG	329	0.1001549509727816	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	3.0442234338231487E-4	0.0	0.0	0.0
6	0.0	3.0442234338231487E-4	0.0	0.0	0.0
7	0.0	3.0442234338231487E-4	0.0	0.0	0.0
8	0.0	3.0442234338231487E-4	0.0	0.0	0.0
9	0.0	3.0442234338231487E-4	0.0	0.0	0.0
10	0.0	3.0442234338231487E-4	0.0	0.0	0.0
11	0.0	3.0442234338231487E-4	0.0	0.0	0.0
12	0.0	3.0442234338231487E-4	0.0	0.0	0.0
13	0.0	3.0442234338231487E-4	0.0	0.0	0.0
14	0.0	3.0442234338231487E-4	0.0	0.0	0.0
15	0.0	3.0442234338231487E-4	0.0	0.0	0.0
16	0.0	3.0442234338231487E-4	0.0	0.0	0.0
17	0.0	3.0442234338231487E-4	0.0	6.088446867646297E-4	0.0
18	0.0	6.088446867646297E-4	0.0	9.132670301469447E-4	0.0
19	0.0	6.088446867646297E-4	0.0	0.0012176893735292595	0.0
20	0.0	6.088446867646297E-4	0.0	0.0012176893735292595	0.0
21	0.0	6.088446867646297E-4	0.0	0.0021309564036762043	0.0
22	0.0	6.088446867646297E-4	0.0	0.002435378747058519	0.0
23	0.0	6.088446867646297E-4	0.0	0.0036530681205877787	0.0
24	0.0	6.088446867646297E-4	0.0	0.0042619128073524085	0.0
25	0.0	6.088446867646297E-4	0.0	0.006697291554410928	0.0
26	0.0	6.088446867646297E-4	0.0	0.012176893735292595	0.0
27	0.0	6.088446867646297E-4	0.0	0.04170586104337714	0.0
28	0.0	6.088446867646297E-4	0.0	0.15312443872130438	0.0
29	0.0	6.088446867646297E-4	0.0	0.30107369760510944	0.0
30	0.0	6.088446867646297E-4	0.0	0.48311825894773375	0.0
31	0.0	6.088446867646297E-4	0.0	1.1479766568947094	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGACC	25	0.0054906993	29.6	29
TCATACA	25	0.0054906993	29.6	2
TTTCGTC	40	5.9274156E-5	27.750002	17
GGTATCA	170	0.0	27.205883	1
TGTATTG	35	8.855858E-4	26.42857	5
ATTGTTA	45	1.3204027E-4	24.666668	8
CTACTAG	40	0.0019282674	23.125002	1
TTAATAC	50	2.6964117E-4	22.2	3
CTAATTT	50	2.6964117E-4	22.2	29
TTCGTCA	45	0.003820079	20.555557	18
TTTTTCG	45	0.003820079	20.555557	15
CTTGCGC	45	0.003820079	20.555557	3
TCACTAC	45	0.003820079	20.555557	22
TTGTTAT	45	0.003820079	20.555557	9
TGTACCG	45	0.003820079	20.555557	5
GGCTAAT	45	0.003820079	20.555557	27
CCTGTAT	65	6.882966E-5	19.923077	3
TCCTGTA	75	9.23277E-6	19.733334	2
AGTCAGA	50	0.0070244484	18.5	6
GACCCGC	50	0.0070244484	18.5	11
>>END_MODULE