FastQCFastQC Report
Fri 10 Feb 2017
ERR1632199.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632199.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1682218
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA58810.3495979712498618No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC56360.3350338660030983No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG44600.2651261608186335No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG34140.20294634821408403No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA30260.17988156112941367No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC29630.17613650549453164No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC28790.17114309798135557No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA28390.16876528487984316No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT28370.16864639422476754No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG27860.16561468252033923No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT27530.16365298671159148No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA25850.15366617168523938No Hit
GTCTTGAAGGGAACGTTGCCAGCTGCCTTGTACCAGCATTACA24920.14813775622422304No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT24640.14647328705316434No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC24250.14415491927918972No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA23970.14249045010813105No Hit
GTTGAAGAACTTGGCCGCAGACATGCTGATGGTTCTTTCTCTG23110.13737815193987937No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC22580.13422754958037544No Hit
ATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAGGGCAC22410.13321697901223264No Hit
GTGTTCATCTCATCAGAGAAAGAACCATCAGCATGTCTGCGGC22190.13190918180640082No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA21720.12911525141212377No Hit
TTGTAATGCTGGTACAAGGCAGCTGGCAACGTTCCCTTCAAGA20720.12317071865834274No Hit
GGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGATCTGG20180.11996067097130099No Hit
ATTCATTGCTTGGCTGGTGAAAGGCCGAGGAAGGCGAGATTTC20130.11966344433361194No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC20080.11936621769592289No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC19520.11603727935380552No Hit
CTTCAGCATGTCTCTCAAATTCATCGTGACGTTTGGCAATGTT19440.11556171673350302No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG19210.11419447420013339No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC19050.11324334895952844No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA18780.11163832511600755No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT18710.1112222078232429No Hit
GTCCAGATACTTGCTGTAGTCACTGGTGAATGTGCCCTGTGAA18320.10890384004926829No Hit
GATCAGGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGA18290.10872550406665485No Hit
CATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTCTTCACCTGCT18260.10854716808404141No Hit
GGTGAATGTGCCCTGTGAATGGCGCTTGTCCTCGTTCATCTGA18120.10771493349851209No Hit
GAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGCT18080.10747715218836083No Hit
TGCTGGTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGA17680.10509933908684843No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG17560.10438599515639471No Hit
CTGGTGAATGTGCCCTGTGAATGGCGCTTGTCCTCGTTCATCT17490.10396987786363003No Hit
CTGGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGAC16890.10040315821136143No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAC303.6001825E-430.83333225
GGTATCA5800.029.9827611
TAAGACG454.009711E-628.7777774
TATACTG701.924036E-723.7857135
TTAGTAC400.001931557223.1250023
TCGCTAT502.702824E-422.211
ACCGTTA450.003826534420.5555558
GGTCTAT555.144457E-420.1818181
CTTATAC13000.019.06923137
TAATATA609.2392444E-418.54
TTCCTCG15500.018.38064419
AAATCTC15550.018.3215459
CGGCCTT15200.018.25657824
CTTAGGA11600.018.0215532
ATCTCGC16000.017.80625211
GAAATCT16000.017.6906268
GCTATTA1054.8005313E-717.61904736
CTTAATA953.6083984E-617.5263162
TATATAC953.6083984E-617.5263163
TATAGAG852.7243308E-517.4117642
Produced by FastQC (version 0.11.5)