##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632199.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1682218
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.404158676223894	33.0	31.0	34.0	31.0	34.0
2	32.592102807127254	34.0	31.0	34.0	31.0	34.0
3	32.671509281198986	34.0	31.0	34.0	31.0	34.0
4	36.19967982746588	37.0	35.0	37.0	35.0	37.0
5	35.98067254065763	37.0	35.0	37.0	35.0	37.0
6	36.00380390650914	37.0	35.0	37.0	35.0	37.0
7	35.97749934907366	37.0	35.0	37.0	35.0	37.0
8	35.95714824119109	37.0	35.0	37.0	35.0	37.0
9	37.68896896835012	39.0	37.0	39.0	35.0	39.0
10	37.620456445002965	39.0	37.0	39.0	35.0	39.0
11	37.69612261906602	39.0	37.0	39.0	35.0	39.0
12	37.658343330055914	39.0	37.0	39.0	35.0	39.0
13	37.676509822151466	39.0	37.0	39.0	35.0	39.0
14	38.992055131974574	40.0	38.0	41.0	36.0	41.0
15	39.00400720952933	40.0	38.0	41.0	36.0	41.0
16	38.99038650163059	40.0	38.0	41.0	36.0	41.0
17	38.974027741945456	40.0	38.0	41.0	36.0	41.0
18	38.951082439969134	40.0	38.0	41.0	36.0	41.0
19	38.99938890203291	40.0	38.0	41.0	36.0	41.0
20	38.97297437074148	40.0	38.0	41.0	36.0	41.0
21	38.94398466786112	40.0	38.0	41.0	36.0	41.0
22	38.92600780636041	40.0	38.0	41.0	36.0	41.0
23	38.87803602149067	40.0	38.0	41.0	35.0	41.0
24	38.8361395490953	40.0	38.0	41.0	35.0	41.0
25	38.77557724385306	40.0	38.0	41.0	35.0	41.0
26	38.67889892986521	40.0	38.0	41.0	35.0	41.0
27	38.59913875609463	40.0	38.0	41.0	35.0	41.0
28	38.550131433619185	40.0	38.0	41.0	35.0	41.0
29	38.49450071274948	40.0	38.0	41.0	34.0	41.0
30	38.43455604446035	40.0	38.0	41.0	34.0	41.0
31	38.35674329961991	40.0	38.0	41.0	34.0	41.0
32	38.266395318561564	40.0	38.0	41.0	34.0	41.0
33	38.20669378166207	40.0	38.0	41.0	34.0	41.0
34	38.13715463750834	40.0	38.0	41.0	34.0	41.0
35	38.13196208814791	40.0	38.0	41.0	34.0	41.0
36	38.09260095897202	40.0	38.0	41.0	34.0	41.0
37	38.05657114595136	40.0	38.0	41.0	33.0	41.0
38	38.003327154982294	40.0	38.0	41.0	33.0	41.0
39	37.964254335644966	40.0	37.0	41.0	33.0	41.0
40	37.92878806432935	40.0	37.0	41.0	33.0	41.0
41	37.901433702409555	40.0	37.0	41.0	33.0	41.0
42	37.87916310490079	40.0	37.0	41.0	33.0	41.0
43	36.99402336676935	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	0.0
15	4.0
16	8.0
17	17.0
18	32.0
19	93.0
20	148.0
21	281.0
22	486.0
23	854.0
24	1399.0
25	2078.0
26	3406.0
27	4952.0
28	7289.0
29	10426.0
30	14266.0
31	20021.0
32	26821.0
33	37158.0
34	52824.0
35	79105.0
36	126077.0
37	232647.0
38	529497.0
39	532327.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.19435293166522	18.21933899173591	12.965085381323943	26.621222695274927
2	17.928948566713707	20.980931127832424	35.31676631685073	25.77335398860314
3	19.43582817446966	21.88295452789115	31.483018253282275	27.19819904435691
4	15.519688886933796	15.634121142444082	33.41409971834804	35.43209025227408
5	14.855209015716156	36.62438518669994	33.828730877924265	14.69167491965964
6	35.02625700117345	34.39048922315657	15.848837665510654	14.734416110159327
7	29.670767997964592	30.817587256824027	20.396107995515443	19.115536749695938
8	27.070867152770926	34.53749751815757	20.098703021843782	18.29293230722772
9	26.54792660642081	15.260507258868946	18.45985478695389	39.73171134775635
10	16.651765704563857	27.704554344323984	32.47914360683336	23.1645363442788
11	36.15607489635707	21.307404866670073	21.78909035570895	20.747429881263905
12	22.414752428044405	24.828589398044723	27.93865004416788	24.818008129742992
13	30.5641123802028	18.785139619240788	24.767301265353243	25.883446735203165
14	23.10681493123959	19.57498968623567	23.93958452471677	33.37861085780796
15	25.892660760971527	27.164731324953127	21.828324271883908	25.11428364219144
16	26.394973778666024	25.561253059948235	22.334085118575594	25.709688042810146
17	25.34885490465564	25.925534027099932	23.581545316956543	25.14406575128788
18	24.003131579854692	24.904798307948198	25.444383546008897	25.64768656618821
19	26.048466964448124	24.992777392704156	25.37376249689398	23.584993145953735
20	25.985752143895734	24.20049006728022	25.4607310110818	24.353026777742244
21	25.403604051317963	25.64495208112147	23.436855389729512	25.51458847783105
22	25.77775294284094	24.588489720119508	23.760891870138114	25.872865466901434
23	24.67373431980873	25.535751014434517	24.214459719251607	25.57605494650515
24	27.089770766927952	24.479229208105014	23.71214670155711	24.718853323409924
25	26.125151436971905	24.035053720742496	24.480774786620994	25.359020055664605
26	25.9683941082547	23.734260363401177	24.298396521734993	25.99894900660913
27	25.357177250510933	24.0474777941979	24.593839799597912	26.001505155693255
28	24.616488469389814	24.771105766315664	24.932856502546045	25.67954926174848
29	25.04734820338387	25.50406665485686	24.379539393824107	25.069045747935164
30	24.439281947999607	26.32096434588145	23.92769545920921	25.312058246909736
31	25.58622009751412	24.733714655294378	24.930716470754682	24.749348776436822
32	24.102761948808062	25.203986641445997	24.992420720738927	25.700830689007013
33	25.03040628503559	24.18122978115797	24.987011195932986	25.80135273787345
34	24.936066550233086	24.25619033918315	25.046872640763564	25.7608704698202
35	24.857420381900564	24.166309003945983	24.851951411767086	26.124319202386374
36	24.62136298624792	24.501402315276614	25.306173159483492	25.571061538991973
37	25.944794313222186	22.348351997184672	25.195129287642864	26.511724401950282
38	24.324255239213944	23.3454879213039	26.84158652445759	25.48867031502457
39	24.26819829534579	22.55355726784519	27.270662898625503	25.907581538183518
40	23.77622876464287	22.25734120072428	28.317376225911268	25.64905380872158
41	22.89067172031211	22.80090927573002	28.851908611131254	25.456510392826615
42	23.52209998941873	21.358171176387366	28.615910660806147	26.503818173387756
43	21.811025681570403	21.93895202643177	29.455754248260334	26.794268043737496
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9.0
1	13.5
2	18.0
3	52.5
4	87.0
5	87.0
6	110.0
7	133.0
8	157.5
9	182.0
10	346.5
11	511.0
12	511.0
13	710.5
14	910.0
15	2018.0
16	3126.0
17	3494.0
18	3862.0
19	3862.0
20	4360.0
21	4858.0
22	6771.0
23	8684.0
24	10481.0
25	12278.0
26	12278.0
27	14513.0
28	16748.0
29	24632.5
30	32517.0
31	34907.0
32	37297.0
33	37297.0
34	43540.5
35	49784.0
36	53380.0
37	56976.0
38	72059.0
39	87142.0
40	87142.0
41	99657.0
42	112172.0
43	105787.0
44	99402.0
45	109460.0
46	119518.0
47	119518.0
48	127187.0
49	134856.0
50	146111.5
51	157367.0
52	168125.5
53	178884.0
54	178884.0
55	149493.5
56	120103.0
57	110582.0
58	101061.0
59	90453.5
60	79846.0
61	79846.0
62	74529.5
63	69213.0
64	62396.0
65	55579.0
66	48855.0
67	42131.0
68	42131.0
69	36907.5
70	31684.0
71	27731.5
72	23779.0
73	19888.0
74	15997.0
75	15997.0
76	13000.5
77	10004.0
78	8420.0
79	6836.0
80	5480.5
81	4125.0
82	4125.0
83	3250.0
84	2375.0
85	1798.0
86	1221.0
87	904.0
88	587.0
89	587.0
90	431.0
91	275.0
92	165.5
93	56.0
94	37.0
95	18.0
96	18.0
97	11.0
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1682218.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.568734969201415
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.67382296202702	43.82985582134228
2	7.945941160774004	8.036323852800985
3	2.0688064171467837	3.138507702338366
4	0.9234965821972495	1.8680021564038414
5	0.5147876794741699	1.3016080864369752
6	0.3243208501970153	0.9840297071159364
7	0.21540811403952514	0.7625041080356183
8	0.1652130753702559	0.6683692977476133
9	0.1213250534672009	0.5521729026516365
>10	0.8105085612080352	8.064767119628707
>50	0.11030078208511503	3.898113796919842
>100	0.09652334515601517	10.532178822619803
>500	0.018598310213225524	6.524819138766894
>1k	0.010711684996224826	9.153199505426485
>5k	2.354216482686775E-4	0.6855479817650716
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	5881	0.3495979712498618	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	5636	0.3350338660030983	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	4460	0.2651261608186335	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	3414	0.20294634821408403	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	3026	0.17988156112941367	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	2963	0.17613650549453164	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	2879	0.17114309798135557	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	2839	0.16876528487984316	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	2837	0.16864639422476754	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	2786	0.16561468252033923	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	2753	0.16365298671159148	No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA	2585	0.15366617168523938	No Hit
GTCTTGAAGGGAACGTTGCCAGCTGCCTTGTACCAGCATTACA	2492	0.14813775622422304	No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT	2464	0.14647328705316434	No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC	2425	0.14415491927918972	No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA	2397	0.14249045010813105	No Hit
GTTGAAGAACTTGGCCGCAGACATGCTGATGGTTCTTTCTCTG	2311	0.13737815193987937	No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC	2258	0.13422754958037544	No Hit
ATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAGGGCAC	2241	0.13321697901223264	No Hit
GTGTTCATCTCATCAGAGAAAGAACCATCAGCATGTCTGCGGC	2219	0.13190918180640082	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	2172	0.12911525141212377	No Hit
TTGTAATGCTGGTACAAGGCAGCTGGCAACGTTCCCTTCAAGA	2072	0.12317071865834274	No Hit
GGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGATCTGG	2018	0.11996067097130099	No Hit
ATTCATTGCTTGGCTGGTGAAAGGCCGAGGAAGGCGAGATTTC	2013	0.11966344433361194	No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC	2008	0.11936621769592289	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1952	0.11603727935380552	No Hit
CTTCAGCATGTCTCTCAAATTCATCGTGACGTTTGGCAATGTT	1944	0.11556171673350302	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	1921	0.11419447420013339	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1905	0.11324334895952844	No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA	1878	0.11163832511600755	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	1871	0.1112222078232429	No Hit
GTCCAGATACTTGCTGTAGTCACTGGTGAATGTGCCCTGTGAA	1832	0.10890384004926829	No Hit
GATCAGGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGA	1829	0.10872550406665485	No Hit
CATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTCTTCACCTGCT	1826	0.10854716808404141	No Hit
GGTGAATGTGCCCTGTGAATGGCGCTTGTCCTCGTTCATCTGA	1812	0.10771493349851209	No Hit
GAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGCT	1808	0.10747715218836083	No Hit
TGCTGGTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGA	1768	0.10509933908684843	No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG	1756	0.10438599515639471	No Hit
CTGGTGAATGTGCCCTGTGAATGGCGCTTGTCCTCGTTCATCT	1749	0.10396987786363003	No Hit
CTGGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGAC	1689	0.10040315821136143	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.161172927646714E-4	0.0
3	0.0	0.0	0.0	5.350079478402918E-4	5.94453275378102E-5
4	0.0	0.0	0.0	7.727892579915326E-4	5.94453275378102E-5
5	0.0	0.0	0.0	8.322345855293428E-4	5.94453275378102E-5
6	0.0	0.0	0.0	8.91679913067153E-4	5.94453275378102E-5
7	0.0	0.0	0.0	9.511252406049632E-4	5.94453275378102E-5
8	0.0	0.0	0.0	9.511252406049632E-4	5.94453275378102E-5
9	0.0	0.0	0.0	0.0016050238435208755	5.94453275378102E-5
10	0.0	0.0	0.0	0.0022589224464367877	5.94453275378102E-5
11	0.0	0.0	0.0	0.0024372584290502184	5.94453275378102E-5
12	0.0	0.0	0.0	0.0024967037565880286	5.94453275378102E-5
13	0.0	0.0	0.0	0.0026155944116636486	5.94453275378102E-5
14	0.0	0.0	0.0	0.002734485066739269	5.94453275378102E-5
15	0.0	0.0	0.0	0.0029128210493526998	5.94453275378102E-5
16	0.0	0.0	0.0	0.00297226637689051	5.94453275378102E-5
17	0.0	0.0	0.0	0.003210047687041751	5.94453275378102E-5
18	0.0	0.0	0.0	0.003447828997192992	5.94453275378102E-5
19	0.0	0.0	0.0	0.003507274324730802	5.94453275378102E-5
20	0.0	0.0	0.0	0.003923391617495473	5.94453275378102E-5
21	0.0	0.0	0.0	0.004161172927646714	5.94453275378102E-5
22	0.0	0.0	0.0	0.005409524805940728	5.94453275378102E-5
23	0.0	0.0	0.0	0.0058256420987053996	5.94453275378102E-5
24	0.0	0.0	0.0	0.007014548649461604	5.94453275378102E-5
25	0.0	0.0	0.0	0.008084564545142187	5.94453275378102E-5
26	0.0	0.0	0.0	0.010343486991578974	5.94453275378102E-5
27	0.0	0.0	0.0	0.02419424830788875	5.94453275378102E-5
28	0.0	0.0	0.0	0.08280734126016961	5.94453275378102E-5
29	0.0	0.0	0.0	0.1772065213902122	5.94453275378102E-5
30	0.0	0.0	0.0	0.29514605122522763	5.94453275378102E-5
31	0.0	0.0	0.0	0.7236874174453014	5.94453275378102E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTAC	30	3.6001825E-4	30.833332	25
GGTATCA	580	0.0	29.982761	1
TAAGACG	45	4.009711E-6	28.777777	4
TATACTG	70	1.924036E-7	23.785713	5
TTAGTAC	40	0.0019315572	23.125002	3
TCGCTAT	50	2.702824E-4	22.2	11
ACCGTTA	45	0.0038265344	20.555555	8
GGTCTAT	55	5.144457E-4	20.181818	1
CTTATAC	1300	0.0	19.069231	37
TAATATA	60	9.2392444E-4	18.5	4
TTCCTCG	1550	0.0	18.380644	19
AAATCTC	1555	0.0	18.321545	9
CGGCCTT	1520	0.0	18.256578	24
CTTAGGA	1160	0.0	18.021553	2
ATCTCGC	1600	0.0	17.806252	11
GAAATCT	1600	0.0	17.690626	8
GCTATTA	105	4.8005313E-7	17.619047	36
CTTAATA	95	3.6083984E-6	17.526316	2
TATATAC	95	3.6083984E-6	17.526316	3
TATAGAG	85	2.7243308E-5	17.411764	2
>>END_MODULE