##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632198.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	116220
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.697539149888144	34.0	31.0	34.0	31.0	34.0
2	32.868757528824645	34.0	31.0	34.0	31.0	34.0
3	32.9547065909482	34.0	31.0	34.0	31.0	34.0
4	36.37175184993977	37.0	37.0	37.0	35.0	37.0
5	36.221295818275685	37.0	37.0	37.0	35.0	37.0
6	36.25324384787472	37.0	37.0	37.0	35.0	37.0
7	36.22757700912064	37.0	36.0	37.0	35.0	37.0
8	36.21074685940458	37.0	36.0	37.0	35.0	37.0
9	37.998993288590604	39.0	38.0	39.0	35.0	39.0
10	37.954887282739634	39.0	38.0	39.0	35.0	39.0
11	38.03914128377215	39.0	38.0	39.0	35.0	39.0
12	37.9867234555154	39.0	38.0	39.0	35.0	39.0
13	38.01497160557563	39.0	38.0	39.0	35.0	39.0
14	39.370160041300984	40.0	39.0	41.0	37.0	41.0
15	39.39225606608157	40.0	39.0	41.0	37.0	41.0
16	39.388074341765616	40.0	39.0	41.0	37.0	41.0
17	39.37120977456548	40.0	39.0	41.0	37.0	41.0
18	39.354809843400446	40.0	39.0	41.0	36.0	41.0
19	39.376527275856134	40.0	39.0	41.0	37.0	41.0
20	39.35629839958699	40.0	39.0	41.0	37.0	41.0
21	39.32265530889692	40.0	39.0	41.0	36.0	41.0
22	39.284658406470484	40.0	39.0	41.0	36.0	41.0
23	39.236792290483564	40.0	39.0	41.0	36.0	41.0
24	39.18295474100843	40.0	39.0	41.0	36.0	41.0
25	39.12691447255206	40.0	39.0	41.0	36.0	41.0
26	39.03580278781621	40.0	39.0	41.0	36.0	41.0
27	38.96171054895887	40.0	38.0	41.0	35.0	41.0
28	38.889821029082775	40.0	38.0	41.0	35.0	41.0
29	38.822328342798144	40.0	38.0	41.0	35.0	41.0
30	38.751187403200824	40.0	38.0	41.0	35.0	41.0
31	38.67473756668387	40.0	38.0	41.0	35.0	41.0
32	38.59302185510239	40.0	38.0	41.0	35.0	41.0
33	38.52318877990019	40.0	38.0	41.0	35.0	41.0
34	38.437996902426434	40.0	38.0	41.0	34.0	41.0
35	38.429444157632076	40.0	38.0	41.0	34.0	41.0
36	38.398339356393045	40.0	38.0	41.0	34.0	41.0
37	38.34355532610566	40.0	38.0	41.0	34.0	41.0
38	38.29818447771468	40.0	38.0	41.0	34.0	41.0
39	38.24241954913096	40.0	38.0	41.0	34.0	41.0
40	38.176845637583895	40.0	37.0	41.0	34.0	41.0
41	38.1358802271554	40.0	37.0	41.0	34.0	41.0
42	38.11846498020995	40.0	37.0	41.0	34.0	41.0
43	37.20688349681638	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	0.0
17	0.0
18	1.0
19	1.0
20	10.0
21	13.0
22	26.0
23	30.0
24	56.0
25	125.0
26	173.0
27	280.0
28	369.0
29	563.0
30	753.0
31	1008.0
32	1514.0
33	2047.0
34	3153.0
35	4611.0
36	7565.0
37	13331.0
38	31527.0
39	49063.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.26966098778179	16.911030803648252	11.759593873687834	28.05971433488212
2	20.56960936155567	18.80055067974531	31.593529512992603	29.03631044570642
3	21.294957838582	19.42694889003614	27.686284632593356	31.591808638788503
4	16.544484598175874	14.452762003097575	30.850972293925317	38.151781104801245
5	18.221476510067113	32.596799173980386	31.902426432627774	17.27929788332473
6	38.9244536224402	31.203751505764927	14.188607812768886	15.683187059025986
7	32.924625709860614	26.969540526587505	19.04405437962485	21.061779383927036
8	29.836516950610907	30.53433144037171	18.355704697986578	21.273446911030806
9	27.905696093615557	13.151781104801238	17.36706246773361	41.5754603338496
10	19.13440027533987	24.061263121665807	30.43107898812597	26.37325761486835
11	40.13164687661332	19.083634486319053	18.87540870762347	21.909309929444156
12	24.7212183789365	22.25864739287558	26.241610738255034	26.778523489932887
13	34.075030115298574	16.868008948545864	21.164171399070728	27.892789537084838
14	25.493030459473413	17.94269488900361	21.672689726381	34.89158492514197
15	28.377215625537772	24.24367578729995	19.124935467217348	28.25417311994493
16	28.459817587334367	22.47031491997935	20.973154362416107	28.096713130270178
17	27.043538117363617	23.056272586473927	22.12011701944588	27.780072276716574
18	27.53914988814318	21.979005334710035	22.837721562553778	27.644123214593012
19	27.98313543279986	22.283600068834968	22.16744106005851	27.56582343830666
20	28.318705902598516	21.9316812940974	21.972982274995697	27.77663052830838
21	28.83582860092927	21.99191189124075	21.587506453278266	27.584753054551715
22	28.97091722595078	21.838754087076236	21.63741180519704	27.55291688177594
23	28.187919463087248	21.692479779728103	21.686456720013766	28.433144037170884
24	27.901393908105316	21.870590259852005	21.604715195319223	28.623300636723453
25	27.895370848390982	21.65806229564619	21.78024436413698	28.666322491825845
26	27.48236103940802	22.022027189812423	22.198416795732232	28.297194975047326
27	27.57442780932714	22.025468938220616	21.901565995525726	28.49853725692652
28	27.61228704181724	22.00051626226123	22.151953192221647	28.23524350369988
29	27.20185854414042	22.329203235243504	22.455687489244536	28.013250731371535
30	27.286181380141112	22.36620203063156	22.34641197728446	28.001204611942864
31	27.767165720185854	22.181208053691275	22.010841507485804	28.04078471863707
32	26.939425228015836	22.136465324384787	22.256926518671484	28.667182928927897
33	26.50662536568577	21.824126656341424	23.085527447943555	28.583720530029254
34	27.133884013078646	21.48167268972638	23.027017725004303	28.357425572190675
35	26.65376011013595	21.560832903114783	23.934778867664775	27.850628119084497
36	26.491997934950955	21.105661676131472	24.050077439339184	28.35226294957839
37	25.748580278781617	20.401824126656344	25.053347100326967	28.796248494235073
38	25.150576492858374	19.969024264326276	26.460161762175183	28.420237480640164
39	24.96730339012218	19.340905179831356	27.18723111340561	28.504560316640852
40	24.25830321803476	19.526759593873688	28.349681638272244	27.865255549819306
41	23.477026329375324	19.53020134228188	28.941662364481157	28.05110996386164
42	22.648425400103253	19.654104284976768	29.55687489244536	28.140595422474618
43	21.915332989158493	19.02856651178799	30.126484254001035	28.929616245052486
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.5
2	3.0
3	1.5
4	0.0
5	0.0
6	1.5
7	3.0
8	3.5
9	4.0
10	5.5
11	7.0
12	7.0
13	12.5
14	18.0
15	31.0
16	44.0
17	50.0
18	56.0
19	56.0
20	80.0
21	104.0
22	105.5
23	107.0
24	163.5
25	220.0
26	220.0
27	325.5
28	431.0
29	556.0
30	681.0
31	903.5
32	1126.0
33	1126.0
34	1400.5
35	1675.0
36	2059.5
37	2444.0
38	2903.5
39	3363.0
40	3363.0
41	3931.5
42	4500.0
43	5032.0
44	5564.0
45	6171.5
46	6779.0
47	6779.0
48	7417.0
49	8055.0
50	8592.0
51	9129.0
52	9637.0
53	10145.0
54	10145.0
55	10244.0
56	10343.0
57	10338.0
58	10333.0
59	10096.0
60	9859.0
61	9859.0
62	9229.5
63	8600.0
64	7781.0
65	6962.0
66	6140.5
67	5319.0
68	5319.0
69	4538.0
70	3757.0
71	3156.0
72	2555.0
73	2072.0
74	1589.0
75	1589.0
76	1322.5
77	1056.0
78	832.5
79	609.0
80	513.5
81	418.0
82	418.0
83	300.5
84	183.0
85	149.0
86	115.0
87	80.5
88	46.0
89	46.0
90	29.5
91	13.0
92	8.5
93	4.0
94	2.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	116220.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.02193976606469
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.50098036450999	82.30137661712894
2	4.28399910034717	7.541718215372678
3	1.020020543901222	2.6935256062626567
4	0.4300001484655224	1.5139778867052434
5	0.22299995654689522	0.9814444371502927
6	0.1199999748492016	0.6337578334863421
7	0.11099997669091728	0.6839303283625754
8	0.07899998340978764	0.556298542497315
9	0.04099999138987755	0.32480088952760755
>10	0.18899996030943397	2.6609026803304863
>50	0.00199999957999401	0.10826696317586827
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	8.604371020478403E-4	0.0	0.0	0.0
5	0.0	8.604371020478403E-4	0.0	0.0	0.0
6	0.0	8.604371020478403E-4	0.0	0.0	0.0
7	0.0	8.604371020478403E-4	0.0	0.0	0.0
8	0.0	8.604371020478403E-4	0.0	0.0	0.0
9	0.0	8.604371020478403E-4	0.0	0.0	0.0
10	0.0	8.604371020478403E-4	0.0	0.0	0.0
11	0.0	0.002581311306143521	0.0	0.0	0.0
12	0.0	0.002581311306143521	0.0	0.0	0.0
13	0.0	0.002581311306143521	0.0	0.0	0.0
14	0.0	0.002581311306143521	0.0	0.0	0.0
15	0.0	0.002581311306143521	0.0	0.0	0.0
16	0.0	0.003441748408191361	0.0	0.0	0.0
17	0.0	0.003441748408191361	0.0	8.604371020478403E-4	0.0
18	0.0	0.003441748408191361	0.0	8.604371020478403E-4	0.0
19	0.0	0.003441748408191361	0.0	8.604371020478403E-4	0.0
20	0.0	0.003441748408191361	0.0	8.604371020478403E-4	0.0
21	0.0	0.003441748408191361	0.0	8.604371020478403E-4	0.0
22	0.0	0.003441748408191361	0.0	8.604371020478403E-4	0.0
23	0.0	0.003441748408191361	0.0	8.604371020478403E-4	0.0
24	0.0	0.003441748408191361	0.0	0.004302185510239202	0.0
25	0.0	0.003441748408191361	0.0	0.006023059714334882	0.0
26	0.0	0.003441748408191361	0.0	0.012046119428669764	0.0
27	0.0	0.003441748408191361	0.0	0.035277921183961454	0.0
28	0.0	0.003441748408191361	0.0	0.1600413009808983	0.0
29	0.0	0.003441748408191361	0.0	0.31491997934950955	0.0
30	0.0	0.003441748408191361	0.0	0.5291688177594218	0.0
31	0.0	0.003441748408191361	0.0	1.0987781793150921	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	35	2.370015E-5	31.714285	1
ATAACGC	25	0.0054762852	29.6	3
TATACAC	40	0.0019208152	23.125002	37
TTATACA	105	3.8198777E-11	22.90476	37
GTATCAA	45	0.0038054576	20.555555	2
TCAACGC	110	3.763671E-8	18.5	3
TCTTATA	290	0.0	17.86207	37
TATCAAC	115	6.2593244E-8	17.695652	1
CAACGCA	120	1.0176154E-7	16.958334	4
ATCAACG	120	1.0176154E-7	16.958334	2
AACGCAG	130	2.53076E-7	15.653845	5
CAGAGTA	155	1.17830496E-7	14.32258	9
CTTATAC	160	1.7366438E-7	13.875001	36
GAGTACA	150	1.2766886E-6	13.566667	11
ACGCAGA	155	1.8460305E-6	13.129032	6
AGAGTAC	155	1.8460305E-6	13.129032	10
AGTACAT	155	1.8460305E-6	13.129032	12
CTCTTAT	455	0.0	12.604395	37
TACATGG	165	3.7205555E-6	12.333333	14
CGCAGAG	165	3.7205555E-6	12.333333	7
>>END_MODULE