FastQCFastQC Report
Fri 10 Feb 2017
ERR1632197.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632197.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256620
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA3050.11885277842724651No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC2970.11573532850128596No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG2650.1032655287974437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1250.026.641
AGAGAGT451.3193031E-424.6666666
TCGCCAT753.7173777E-722.213
TTTACAC502.6941835E-422.23
GAACTTG753.7173777E-722.227
TAGCCTG450.003817835820.5555555
GTTCTAG609.2101895E-418.51
AAATATG609.2101895E-418.57
GCGGCTC500.007020361718.59
GTCCACG500.007020361718.51
GAACAGT609.2101895E-418.56
GCTTTAA1002.8533577E-718.51
GCTTAGG1054.7614776E-717.6190471
GTCGCCA953.5843495E-617.52631612
GTATCAA1900.017.5263162
TTCTAGA852.709793E-517.4117642
ACACTAC752.0593924E-417.26666611
TAGGACA1401.8462742E-917.1785724
TCTAGAT650.001575417217.0769213
GTAAATG650.001575417217.0769213
Produced by FastQC (version 0.11.5)