##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632197.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	256620
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83272153378536	34.0	31.0	34.0	31.0	34.0
2	32.99400670251734	34.0	33.0	34.0	31.0	34.0
3	33.078711713818095	34.0	33.0	34.0	31.0	34.0
4	36.4618735874055	37.0	37.0	37.0	35.0	37.0
5	36.33268256566129	37.0	37.0	37.0	35.0	37.0
6	36.36190476190476	37.0	37.0	37.0	35.0	37.0
7	36.33746005767282	37.0	37.0	37.0	35.0	37.0
8	36.32299509001637	37.0	37.0	37.0	35.0	37.0
9	38.124635648039906	39.0	39.0	39.0	37.0	39.0
10	38.09222196243473	39.0	38.0	39.0	37.0	39.0
11	38.16810848725742	39.0	39.0	39.0	37.0	39.0
12	38.13741719273634	39.0	38.0	39.0	37.0	39.0
13	38.147506040059234	39.0	39.0	39.0	37.0	39.0
14	39.574452497856754	41.0	39.0	41.0	37.0	41.0
15	39.58542981840854	41.0	39.0	41.0	37.0	41.0
16	39.58580780921206	41.0	39.0	41.0	37.0	41.0
17	39.576478840308624	41.0	39.0	41.0	37.0	41.0
18	39.56652248460759	41.0	39.0	41.0	37.0	41.0
19	39.584553035616864	41.0	39.0	41.0	37.0	41.0
20	39.56464032421479	41.0	39.0	41.0	37.0	41.0
21	39.541937495128984	41.0	39.0	41.0	37.0	41.0
22	39.50865092354454	41.0	39.0	41.0	37.0	41.0
23	39.46925804691762	40.0	39.0	41.0	37.0	41.0
24	39.43124074507053	41.0	39.0	41.0	37.0	41.0
25	39.374596679915825	40.0	39.0	41.0	37.0	41.0
26	39.31253994232718	40.0	39.0	41.0	36.0	41.0
27	39.23733925648819	40.0	39.0	41.0	36.0	41.0
28	39.17606188138103	40.0	39.0	41.0	36.0	41.0
29	39.130979658639234	40.0	39.0	41.0	36.0	41.0
30	39.05973813420622	40.0	39.0	41.0	36.0	41.0
31	38.99286103966955	40.0	39.0	41.0	35.0	41.0
32	38.927297170914194	40.0	38.0	41.0	35.0	41.0
33	38.85944197646325	40.0	38.0	41.0	35.0	41.0
34	38.79412750370197	40.0	38.0	41.0	35.0	41.0
35	38.76663938897981	40.0	38.0	41.0	35.0	41.0
36	38.72816616008105	40.0	38.0	41.0	35.0	41.0
37	38.68665341750448	40.0	38.0	41.0	35.0	41.0
38	38.63453355155483	40.0	38.0	41.0	35.0	41.0
39	38.587440573610785	40.0	38.0	41.0	35.0	41.0
40	38.5367703218767	40.0	38.0	41.0	35.0	41.0
41	38.505650377990804	40.0	38.0	41.0	35.0	41.0
42	38.465544384693324	40.0	38.0	41.0	35.0	41.0
43	37.605712726989324	39.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	2.0
19	8.0
20	16.0
21	22.0
22	69.0
23	68.0
24	123.0
25	215.0
26	317.0
27	444.0
28	609.0
29	933.0
30	1261.0
31	1778.0
32	2606.0
33	3724.0
34	5509.0
35	8366.0
36	14019.0
37	25681.0
38	65339.0
39	125508.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.15330060010911	17.309251032655286	12.046995557633855	27.490452809601745
2	18.90694411971008	20.01909438079651	33.7273010677266	27.346660431766818
3	19.634478996181123	20.842880523731587	29.89595510872107	29.626685371366225
4	15.401371677967424	15.03156418050035	33.035227184163354	36.531836957368874
5	15.951211908658717	34.880367859091265	33.467773361390385	15.700646870859636
6	36.37362637362637	33.602213389447435	15.049879198815368	14.974281038110826
7	31.362325617644764	29.47354064375341	19.69799703842257	19.466136700179256
8	28.440105993297482	32.57813108876938	19.46496765645702	19.516795261476112
9	27.502143246824097	13.882004520302393	17.812329514457172	40.80352271841633
10	17.47252747252747	26.14293507910529	31.549762294443145	24.83477515392409
11	37.74764242849349	20.354609929078013	20.385394747096875	21.512352895331617
12	23.48219156729795	23.72184553035617	27.46083703530512	25.33512586704076
13	32.04270906398566	17.862988075754032	23.141220481646013	26.953082378614297
14	24.06866183461928	18.689891668615072	23.336450783259295	33.90499571350635
15	26.838126412594498	25.70298495830411	20.393188371911776	27.06570025718962
16	26.977632296781234	24.543293585846776	21.73914737744525	26.73992673992674
17	25.84521861117606	24.88699244018393	23.18837191177617	26.079417036863845
18	25.72675551398956	23.596368170836257	24.17153768217598	26.50533863299821
19	26.81279713194607	23.648585457096093	24.046060322656068	25.49255708830177
20	27.216506897357963	23.226170992128438	23.61974904528096	25.937573065232638
21	27.3439326630816	23.757696204504715	22.410568155249006	26.48780297716468
22	27.349777881692777	23.426467149871407	22.685293429974283	26.53846153846154
23	26.539240900943028	23.539474709687475	23.076143714441585	26.845140674927908
24	26.987764009040603	23.387888707037643	23.055880289922843	26.568466993998907
25	26.954641103577277	22.847011144883485	23.375029226093055	26.823318525446183
26	26.547813888239418	23.157587093757307	23.313459590055334	26.981139427947937
27	26.562232094146985	23.227340035850673	23.232795573221104	26.977632296781234
28	25.996025251344403	23.685994856207625	23.53986439092822	26.778115501519757
29	25.996414932585143	23.97708674304419	23.61507287039202	26.411425453978644
30	25.878731197880136	24.361702127659573	23.339568233185254	26.419998441275037
31	26.611721611721613	23.830956277764788	23.233574935702595	26.323747174811
32	25.350713116670565	23.884342607746863	23.774452497856753	26.990491777725822
33	25.628945522562546	23.42451874366768	24.19141142545398	26.755124308315796
34	26.22905463330995	23.106149169978956	23.85083002104279	26.813966175668302
35	25.87990024160237	23.209025017535655	24.424051126178785	26.487023614683192
36	25.567765567765566	23.627152988855116	24.268957992362246	26.53612345101707
37	26.238406983087835	21.94295066635492	24.592393422180656	27.226248928376588
38	25.300054555373702	22.416413373860184	25.47424206998675	26.809290000779363
39	25.00857298729639	22.046995557633856	25.974203101862674	26.970228353207077
40	24.496142155716623	21.65536591068506	27.245343309173098	26.60314862442522
41	23.830956277764788	21.804224144649677	27.84116592627231	26.52365365131323
42	23.91356870080274	21.18502065310576	27.965474242069988	26.93593640402151
43	22.989634478996184	21.02681006936326	28.580001558724966	27.403553892915593
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	3.0
2	0.0
3	3.5
4	7.0
5	7.0
6	10.0
7	13.0
8	13.5
9	14.0
10	28.5
11	43.0
12	43.0
13	64.0
14	85.0
15	177.5
16	270.0
17	295.0
18	320.0
19	320.0
20	460.0
21	600.0
22	761.0
23	922.0
24	1198.5
25	1475.0
26	1475.0
27	1733.0
28	1991.0
29	2749.0
30	3507.0
31	4085.0
32	4663.0
33	4663.0
34	5382.0
35	6101.0
36	7136.0
37	8171.0
38	9415.0
39	10659.0
40	10659.0
41	12066.5
42	13474.0
43	13659.0
44	13844.0
45	15048.5
46	16253.0
47	16253.0
48	17164.0
49	18075.0
50	19397.5
51	20720.0
52	22286.5
53	23853.0
54	23853.0
55	22336.5
56	20820.0
57	19959.5
58	19099.0
59	17868.5
60	16638.0
61	16638.0
62	15734.5
63	14831.0
64	13337.0
65	11843.0
66	10425.0
67	9007.0
68	9007.0
69	7776.5
70	6546.0
71	5707.0
72	4868.0
73	3967.5
74	3067.0
75	3067.0
76	2541.5
77	2016.0
78	1669.5
79	1323.0
80	1045.0
81	767.0
82	767.0
83	561.5
84	356.0
85	283.0
86	210.0
87	150.5
88	91.0
89	91.0
90	74.5
91	58.0
92	33.5
93	9.0
94	6.5
95	4.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	256620.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.69805640645787
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.9914980410881	67.05896555140211
2	5.2458834204877265	7.732228244496133
3	1.2980538567798012	2.8699213906673386
4	0.6368472058021329	1.8773760518200269
5	0.37022349120584963	1.3642375868942234
6	0.23733831687537443	1.049482359869707
7	0.17513449278095028	0.9034950209380802
8	0.12551754682207295	0.7400319396554678
9	0.10350872337785892	0.6865552560655702
>10	0.7338792779417295	10.589326264968118
>50	0.06251383204472195	3.0910839054532295
>100	0.019601794793684003	2.0372964277699825
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	305	0.11885277842724651	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	297	0.11573532850128596	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	265	0.1032655287974437	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	3.8968124074507054E-4	0.0
6	0.0	0.0	0.0	3.8968124074507054E-4	0.0
7	0.0	0.0	0.0	3.8968124074507054E-4	0.0
8	0.0	0.0	0.0	3.8968124074507054E-4	0.0
9	0.0	0.0	0.0	0.0011690437222352116	0.0
10	0.0	0.0	0.0	0.0011690437222352116	0.0
11	0.0	0.0	0.0	0.0011690437222352116	0.0
12	0.0	0.0	0.0	0.0011690437222352116	0.0
13	0.0	0.0	0.0	0.0011690437222352116	0.0
14	0.0	0.0	0.0	0.0011690437222352116	0.0
15	0.0	0.0	0.0	0.0015587249629802822	0.0
16	0.0	0.0	0.0	0.0015587249629802822	0.0
17	0.0	0.0	0.0	0.0015587249629802822	0.0
18	0.0	0.0	0.0	0.0019484062037253527	0.0
19	0.0	0.0	0.0	0.0019484062037253527	0.0
20	0.0	0.0	0.0	0.0019484062037253527	0.0
21	0.0	0.0	0.0	0.002727768685215494	0.0
22	0.0	0.0	0.0	0.0035071311667056347	0.0
23	0.0	0.0	0.0	0.0038968124074507055	0.0
24	0.0	0.0	0.0	0.004286493648195775	0.0
25	0.0	0.0	0.0	0.006234899851921129	0.0
26	0.0	0.0	0.0	0.00857298729639155	0.0
27	0.0	0.0	0.0	0.02221183072246902	0.0
28	0.0	0.0	0.0	0.07949497311199438	0.0
29	0.0	3.8968124074507054E-4	0.0	0.16756293352038032	0.0
30	0.0	3.8968124074507054E-4	0.0	0.2813498558179409	0.0
31	0.0	3.8968124074507054E-4	0.0	0.6145273166549762	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	125	0.0	26.64	1
AGAGAGT	45	1.3193031E-4	24.666666	6
TCGCCAT	75	3.7173777E-7	22.2	13
TTTACAC	50	2.6941835E-4	22.2	3
GAACTTG	75	3.7173777E-7	22.2	27
TAGCCTG	45	0.0038178358	20.555555	5
GTTCTAG	60	9.2101895E-4	18.5	1
AAATATG	60	9.2101895E-4	18.5	7
GCGGCTC	50	0.0070203617	18.5	9
GTCCACG	50	0.0070203617	18.5	1
GAACAGT	60	9.2101895E-4	18.5	6
GCTTTAA	100	2.8533577E-7	18.5	1
GCTTAGG	105	4.7614776E-7	17.619047	1
GTCGCCA	95	3.5843495E-6	17.526316	12
GTATCAA	190	0.0	17.526316	2
TTCTAGA	85	2.709793E-5	17.411764	2
ACACTAC	75	2.0593924E-4	17.266666	11
TAGGACA	140	1.8462742E-9	17.178572	4
TCTAGAT	65	0.0015754172	17.076921	3
GTAAATG	65	0.0015754172	17.076921	3
>>END_MODULE