FastQCFastQC Report
Fri 10 Feb 2017
ERR1632196.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632196.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211840
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAATCG250.0054863529.625
GTATTAG250.0054863529.61
TGCGCAA250.0054863529.610
TCTCGTA250.0054863529.628
CGCTAAT250.0054863529.622
CGGACCA358.8454277E-426.4285729
TCAGGGG509.045452E-625.9000023
TATAATC400.001926018623.1253
GGTATCA1450.021.6896551
CTTATAC1400.021.14285937
CGCCTTA450.00381566620.55555525
CGCAAGA450.00381566620.5555552
TGCCGTC450.00381566620.55555535
TCTTATA2750.018.83636537
GCGCCAG609.2029484E-418.511
GGCATCA500.00701641118.517
GTGTTGA650.001574188517.0769231
CTATTGG700.002583143315.8571431
AGGACAG957.016309E-515.5789485
GTATCAA2055.456968E-1215.3414642