##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632196.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	211840
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1859375	33.0	31.0	34.0	30.0	34.0
2	32.38020203927493	34.0	31.0	34.0	31.0	34.0
3	32.4583081570997	34.0	31.0	34.0	31.0	34.0
4	36.05122262084592	37.0	35.0	37.0	35.0	37.0
5	35.757510385196376	37.0	35.0	37.0	35.0	37.0
6	35.764742258308154	37.0	35.0	37.0	35.0	37.0
7	35.73385101963746	37.0	35.0	37.0	33.0	37.0
8	35.703398791540785	37.0	35.0	37.0	33.0	37.0
9	37.431103663142	39.0	37.0	39.0	35.0	39.0
10	37.301147092145015	39.0	37.0	39.0	34.0	39.0
11	37.40609422205438	39.0	37.0	39.0	35.0	39.0
12	37.33131136706949	39.0	37.0	39.0	34.0	39.0
13	37.380456948640486	39.0	37.0	39.0	35.0	39.0
14	38.61389728096677	40.0	38.0	41.0	35.0	41.0
15	38.60072224320242	40.0	38.0	41.0	35.0	41.0
16	38.582491503021146	40.0	38.0	41.0	35.0	41.0
17	38.555027379154076	40.0	38.0	41.0	35.0	41.0
18	38.55855834592145	40.0	38.0	41.0	35.0	41.0
19	38.57375377643505	40.0	38.0	41.0	34.0	41.0
20	38.551052681268885	40.0	38.0	41.0	34.0	41.0
21	38.51670600453172	40.0	38.0	41.0	34.0	41.0
22	38.46661159365559	40.0	38.0	41.0	34.0	41.0
23	38.41983572507553	40.0	38.0	41.0	34.0	41.0
24	38.36748489425982	40.0	38.0	41.0	34.0	41.0
25	38.29563821752266	40.0	38.0	41.0	34.0	41.0
26	38.17288519637462	40.0	38.0	41.0	34.0	41.0
27	38.071360460725074	40.0	38.0	41.0	33.0	41.0
28	37.997205438066466	40.0	38.0	41.0	33.0	41.0
29	37.92508969033233	40.0	37.0	41.0	33.0	41.0
30	37.833610271903325	40.0	37.0	41.0	33.0	41.0
31	37.73329871601209	40.0	37.0	41.0	33.0	41.0
32	37.62725641993958	40.0	37.0	41.0	33.0	41.0
33	37.54037953172205	39.0	37.0	41.0	32.0	41.0
34	37.459757364048336	39.0	37.0	41.0	32.0	41.0
35	37.41034743202417	39.0	37.0	40.0	32.0	41.0
36	37.38789652567976	39.0	36.0	40.0	32.0	41.0
37	37.3188916163142	39.0	36.0	40.0	32.0	41.0
38	37.274867824773416	39.0	36.0	40.0	32.0	41.0
39	37.20972432024169	39.0	36.0	40.0	32.0	41.0
40	37.146950528700906	39.0	36.0	40.0	31.0	41.0
41	37.102700151057405	39.0	36.0	40.0	31.0	41.0
42	37.04793240181269	39.0	36.0	40.0	31.0	41.0
43	36.09743202416919	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	2.0
16	2.0
17	1.0
18	3.0
19	25.0
20	29.0
21	54.0
22	123.0
23	184.0
24	274.0
25	431.0
26	632.0
27	944.0
28	1276.0
29	1886.0
30	2530.0
31	3369.0
32	4608.0
33	6234.0
34	8786.0
35	13068.0
36	20538.0
37	35997.0
38	64938.0
39	45903.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.957892749244714	17.7657666163142	12.281438821752266	26.99490181268882
2	19.833836858006045	20.09252265861027	32.667107250755286	27.406533232628398
3	20.316748489425983	21.000755287009063	29.4845166163142	29.19797960725076
4	15.888406344410877	15.093466767371602	32.84837613293051	36.16975075528701
5	16.505381419939578	34.572318731117825	32.89746978851964	16.02483006042296
6	36.69986782477341	33.51161253776435	14.689388217522659	15.099131419939576
7	31.357628398791544	29.171544561933533	19.51000755287009	19.960819486404834
8	28.985083081570995	31.84242824773414	19.048338368580062	20.124150302114803
9	27.69967900302115	13.598470543806645	17.928625377643503	40.7732250755287
10	17.80730740181269	25.736404833836858	31.658327039274926	24.79796072507553
11	38.83638595166163	19.908893504531722	19.75453172205438	21.500188821752268
12	23.913802870090635	23.75	27.00387084592145	25.332326283987918
13	32.78323262839879	17.854512839879156	22.552870090634443	26.809384441087612
14	24.197035498489424	18.70232250755287	23.022092145015105	34.07854984894259
15	27.44099320241692	25.31155589123867	20.38425226586103	26.863198640483382
16	27.031722054380662	24.19325906344411	21.78247734138973	26.992541540785496
17	25.787858761329307	24.385385196374624	23.305324773413897	26.52143126888217
18	25.77605740181269	23.123583836858007	24.31127265861027	26.789086102719033
19	26.83345921450151	23.555513595166165	23.976586102719033	25.63444108761329
20	27.31589879154079	23.00934667673716	23.46771148036254	26.20704305135952
21	27.59441087613293	23.45496601208459	22.676076283987914	26.27454682779456
22	27.821941087613293	23.23074018126888	22.578833081571	26.36848564954683
23	26.82118580060423	23.289746978851962	22.97866314199396	26.91040407854985
24	26.92692598187311	23.13727341389728	23.011234894259818	26.924565709969787
25	26.76925981873112	22.759157854984892	23.17267749244713	27.298904833836858
26	26.559195619335345	22.90879909365559	23.321374622356494	27.21063066465257
27	26.814577039274923	22.84365558912387	23.34120090634441	27.0005664652568
28	26.34016238670695	23.130664652567976	23.464407099697883	27.06476586102719
29	26.0470166163142	23.578172205438065	23.792484894259818	26.582326283987918
30	26.03993580060423	23.797677492447132	23.504059667673715	26.658327039274926
31	26.655966767371602	23.478568731117825	23.202416918429	26.66304758308157
32	25.63208081570997	23.680135951661633	23.766049848942597	26.921733383685805
33	25.94174848942598	23.150962990936556	23.982722809667674	26.924565709969787
34	26.40105740181269	22.908327039274926	24.15691087613293	26.533704682779458
35	26.063538519637465	22.914463746223564	24.455721299093653	26.566276435045317
36	25.58345921450151	23.457326283987914	24.23196752265861	26.727246978851966
37	25.994146525679756	22.350830815709973	24.62046827794562	27.034554380664655
38	25.15813821752266	22.61046072507553	25.358289274924473	26.873111782477345
39	24.89284365558912	22.16720166163142	26.06920317220544	26.87075151057402
40	24.501982628398792	21.820713746223568	27.048243957703928	26.629059667673715
41	24.019070996978854	21.62056268882175	27.60998867069486	26.75037764350453
42	24.050226586102717	21.25519259818731	27.824301359516618	26.87027945619335
43	22.863953927492446	21.015861027190333	28.56542673716012	27.5547583081571
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	7.0
4	12.0
5	12.0
6	22.0
7	32.0
8	29.0
9	26.0
10	40.5
11	55.0
12	55.0
13	101.5
14	148.0
15	281.5
16	415.0
17	390.5
18	366.0
19	366.0
20	470.0
21	574.0
22	629.0
23	684.0
24	891.5
25	1099.0
26	1099.0
27	1364.0
28	1629.0
29	2168.0
30	2707.0
31	3263.0
32	3819.0
33	3819.0
34	4534.0
35	5249.0
36	6014.5
37	6780.0
38	7673.0
39	8566.0
40	8566.0
41	9527.0
42	10488.0
43	11014.5
44	11541.0
45	12336.5
46	13132.0
47	13132.0
48	13962.0
49	14792.0
50	15569.5
51	16347.0
52	17121.5
53	17896.0
54	17896.0
55	17232.5
56	16569.0
57	16194.0
58	15819.0
59	15000.5
60	14182.0
61	14182.0
62	13434.5
63	12687.0
64	11561.0
65	10435.0
66	9184.0
67	7933.0
68	7933.0
69	6923.5
70	5914.0
71	5147.0
72	4380.0
73	3638.5
74	2897.0
75	2897.0
76	2371.0
77	1845.0
78	1563.5
79	1282.0
80	1020.5
81	759.0
82	759.0
83	581.0
84	403.0
85	320.0
86	237.0
87	166.5
88	96.0
89	96.0
90	65.5
91	35.0
92	20.5
93	6.0
94	4.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	211840.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.92781279957846
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.00165155276652	77.12423550019447
2	4.242124093823084	7.035801454502842
3	1.018315235443821	2.5333996564753156
4	0.48628206228846493	1.6130523131700307
5	0.28120583417026557	1.1659892387110542
6	0.18128975773875863	0.9020377855344424
7	0.1450285706080228	0.8418831507780793
8	0.10825316453501753	0.718175853081752
9	0.07058082870754587	0.526780237526863
>10	0.4510503287834272	6.603842433768752
>50	0.01137485690807795	0.6169129708089823
>100	0.0028437142270194876	0.317889405447442
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	4.720543806646526E-4	0.0	0.0	0.0
17	0.0	4.720543806646526E-4	0.0	0.0	0.0
18	0.0	4.720543806646526E-4	0.0	0.0	0.0
19	4.720543806646526E-4	4.720543806646526E-4	0.0	0.0014161631419939576	0.0
20	4.720543806646526E-4	4.720543806646526E-4	0.0	0.0014161631419939576	0.0
21	4.720543806646526E-4	4.720543806646526E-4	0.0	0.0018882175226586104	0.0
22	4.720543806646526E-4	4.720543806646526E-4	0.0	0.0028323262839879152	0.0
23	4.720543806646526E-4	4.720543806646526E-4	0.0	0.0037764350453172208	0.0
24	4.720543806646526E-4	4.720543806646526E-4	0.0	0.005192598187311178	0.0
25	4.720543806646526E-4	4.720543806646526E-4	0.0	0.0061367069486404835	0.0
26	4.720543806646526E-4	4.720543806646526E-4	0.0	0.0070808157099697885	0.0
27	4.720543806646526E-4	4.720543806646526E-4	0.0	0.02926737160120846	0.0
28	4.720543806646526E-4	4.720543806646526E-4	0.0	0.09393882175226587	0.0
29	4.720543806646526E-4	9.441087613293052E-4	0.0	0.19118202416918428	0.0
30	4.720543806646526E-4	9.441087613293052E-4	0.0	0.30400302114803623	0.0
31	4.720543806646526E-4	9.441087613293052E-4	0.0	0.6198074018126888	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAAATCG	25	0.00548635	29.6	25
GTATTAG	25	0.00548635	29.6	1
TGCGCAA	25	0.00548635	29.6	10
TCTCGTA	25	0.00548635	29.6	28
CGCTAAT	25	0.00548635	29.6	22
CGGACCA	35	8.8454277E-4	26.428572	9
TCAGGGG	50	9.045452E-6	25.900002	3
TATAATC	40	0.0019260186	23.125	3
GGTATCA	145	0.0	21.689655	1
CTTATAC	140	0.0	21.142859	37
CGCCTTA	45	0.003815666	20.555555	25
CGCAAGA	45	0.003815666	20.555555	2
TGCCGTC	45	0.003815666	20.555555	35
TCTTATA	275	0.0	18.836365	37
GCGCCAG	60	9.2029484E-4	18.5	11
GGCATCA	50	0.007016411	18.5	17
GTGTTGA	65	0.0015741885	17.076923	1
CTATTGG	70	0.0025831433	15.857143	1
AGGACAG	95	7.016309E-5	15.578948	5
GTATCAA	205	5.456968E-12	15.341464	2
>>END_MODULE