FastQCFastQC Report
Fri 10 Feb 2017
ERR1632195.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632195.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1587301
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6050.025.3801651
GTCTTAT802.7273927E-823.1250021
TATACAC6000.018.80833437
GTATAGA701.2195687E-418.51
ATAGACG852.7241744E-517.4117643
CTTATAC20300.016.95073937
TATGCCG904.447675E-516.44444533
GTATCAA9100.016.0604382
CTAGACA1751.3278623E-1015.8571424
TATCCCG700.002593077315.8571425
CTTATAG1302.5905683E-715.6538463
TCTTATA33050.014.83358637
TACTATA1001.0937802E-414.82
CATATAC1405.9984814E-714.5357143
CTCGTAT908.2764647E-414.38888829
CTAGAAC1551.210883E-714.322583
ATACACA4400.014.29545437
ATATACT1304.4462395E-614.2307694
TTAGAGT1203.301917E-513.8749994
TATACTG1356.570268E-613.7037045
Produced by FastQC (version 0.11.5)