FastQCFastQC Report
Fri 10 Feb 2017
ERR1632194.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632194.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3001983
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG73970.246403793758992No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT66160.22038765709199554No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG62590.20849551779607012No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA62510.2082290272796348No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC61370.20443153742043177No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC60600.20186656619974197No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG58850.19603708615271973No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA57630.19197310577708135No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC57530.19163999263153722No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT57070.19010767216203422No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG53860.1794147401900677No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC51300.170887043664138No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA50300.1675559122086967No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA49480.16482438441523486No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG48350.1610602058705862No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC48290.16086033798325974No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC47630.1586617912226685No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG47620.15862847990811407No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT47160.15709615943861108No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG47110.156929602865839No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG46870.1561301313165331No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT46480.15483099004891102No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG46470.1547976787343566No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT45580.15183297173901386No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG45220.150633764415055No Hit
GTCCCTGGCTGCTGCCGCGGGGAAGCAGGAACTGGCCAAGTAC42780.14250580366377824No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA42250.14074030399239437No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41600.13857506854635754No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA41320.13764235173883396No Hit
GCCAGGGACTTCTGCAGAAACTGACGGAGTCTGGGGTCCGAGG39910.13294545638666175No Hit
GAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGGCGCCGA38460.1281153157762719No Hit
CTCCAGCTCGGCTTTCGCGGCGCCGAGATGCTGTCCTGCCGCC37550.12508398615182031No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG36800.12258563756023935No Hit
CGGCAGGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAG36170.12048702474331133No Hit
ATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCA36100.12025384554143045No Hit
GTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAGCCAGGGAC35010.11662291225499945No Hit
GTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCATCGTC34870.11615655385123766No Hit
CCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGGCTGCTGCC34730.11569019544747589No Hit
ACGCAAAGCTGGCTGCAAGAATTTCTTCTGGAAGACTTTCACA34090.11355827131599346No Hit
GCCATAGCCGGGTTTGAGTTAGCAGATCTCTGCAGCTCAAGCC32720.10899462122203889No Hit
GTTCGGAGCCATCGCTGCTGCCTGCTGATCCGCGCCTAGAGTT32250.1074289894379815No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC32130.10702925366332854No Hit
GCTTTGCGTTCTCGGGGTGCCATAGCCGGGTTTGAGTTAGCAG31900.10626309342857704No Hit
CTCTCCAGCTCGGCTTTCGCGGCGCCGAGATGCTGTCCTGCCG31880.10619647079946823No Hit
CTGCAGCTCAAGCCTCATTTCATCCTGCTCAGCAGCCTGGGAC31830.10602991422669615No Hit
TAGCAGATCTCTGCAGCTCAAGCCTCATTTCATCCTGCTCAGC31550.10509719741917259No Hit
AGTCTGGGGTCCGAGGGAGCGCCGGTGACACAGCCCAGGGCCA31370.10449759375719317No Hit
CGCCTAGAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGG30290.10089997178531658No Hit
GATCTGCTAACTCAAACCCGGCTATGGCACCCCGAGAACGCAA30200.10060016995432687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19400.023.2680421
CTTATAC40850.019.38310837
ATACACA10050.016.19900537
GTACTAT2700.015.0740751
GTATCAA30550.014.7757782
TATACAC12850.014.39688837
TATACTG2750.014.1272745
GGACCGT1601.7862476E-713.8750016
TCTTATA64400.013.76009437
GAACCGT950.00124589213.6315796
GCGTTAT2900.013.3965511
AAGACGG3200.013.2968765
ATTAGAC1755.312886E-712.6857153
CTCTTAT95450.012.13305537
CGTTATT2901.2732926E-1112.1206892
TTATACA20050.011.99501337
TACGGCT1100.003747457911.7727274
ATCAACG44750.011.7407822
CAACGCA45850.011.5801534
TCCGTAG1603.7673868E-511.56250134
Produced by FastQC (version 0.11.5)