##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632194.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3001983
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.322769316148694	33.0	31.0	34.0	31.0	34.0
2	32.50458713457071	34.0	31.0	34.0	31.0	34.0
3	32.59801204737002	34.0	31.0	34.0	31.0	34.0
4	36.14373599051027	37.0	35.0	37.0	35.0	37.0
5	35.912978188084345	37.0	35.0	37.0	35.0	37.0
6	35.90762639228803	37.0	35.0	37.0	35.0	37.0
7	35.88110758788441	37.0	35.0	37.0	35.0	37.0
8	35.862158446600134	37.0	35.0	37.0	35.0	37.0
9	37.60403606549404	39.0	37.0	39.0	35.0	39.0
10	37.50504716382471	39.0	37.0	39.0	35.0	39.0
11	37.5861045848694	39.0	37.0	39.0	35.0	39.0
12	37.52476446402262	39.0	37.0	39.0	35.0	39.0
13	37.56536795844613	39.0	37.0	39.0	35.0	39.0
14	38.84772898447459	40.0	38.0	41.0	36.0	41.0
15	38.827311480444756	40.0	38.0	41.0	36.0	41.0
16	38.835533712216225	40.0	38.0	41.0	36.0	41.0
17	38.79067070000063	40.0	38.0	41.0	36.0	41.0
18	38.78071994411694	40.0	38.0	41.0	35.0	41.0
19	38.786960818898706	40.0	38.0	41.0	35.0	41.0
20	38.77799974217042	40.0	38.0	41.0	35.0	41.0
21	38.73967773968074	40.0	38.0	41.0	35.0	41.0
22	38.709252850532465	40.0	38.0	41.0	35.0	41.0
23	38.66401875027274	40.0	38.0	41.0	35.0	41.0
24	38.613308269900266	40.0	38.0	41.0	35.0	41.0
25	38.547476118285815	40.0	38.0	41.0	34.0	41.0
26	38.42858270683079	40.0	38.0	41.0	34.0	41.0
27	38.33909052782777	40.0	38.0	41.0	34.0	41.0
28	38.25803577168825	40.0	38.0	41.0	34.0	41.0
29	38.17309325202708	40.0	38.0	41.0	34.0	41.0
30	38.10465015957785	40.0	38.0	41.0	34.0	41.0
31	37.969902561073795	40.0	37.0	41.0	33.0	41.0
32	37.88445570811027	40.0	37.0	41.0	33.0	41.0
33	37.795120758511956	40.0	37.0	41.0	33.0	41.0
34	37.669492132367175	40.0	37.0	41.0	33.0	41.0
35	37.65219190115334	40.0	37.0	41.0	33.0	41.0
36	37.602240252526414	40.0	37.0	41.0	33.0	41.0
37	37.51049622865952	39.0	36.0	41.0	33.0	41.0
38	37.487045063213216	39.0	36.0	41.0	33.0	41.0
39	37.37566735054796	39.0	36.0	41.0	32.0	41.0
40	37.29021683333983	39.0	36.0	41.0	32.0	41.0
41	37.20718072021061	39.0	36.0	41.0	32.0	41.0
42	37.14629929616523	39.0	36.0	40.0	32.0	41.0
43	36.205584775130305	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	1.0
12	7.0
13	1.0
14	4.0
15	12.0
16	17.0
17	30.0
18	65.0
19	140.0
20	291.0
21	527.0
22	1059.0
23	1882.0
24	3085.0
25	4913.0
26	7674.0
27	11500.0
28	16699.0
29	23407.0
30	32457.0
31	43887.0
32	58763.0
33	79540.0
34	112303.0
35	164198.0
36	264597.0
37	476528.0
38	912476.0
39	785914.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.50748055535291	17.705296798815983	11.58933944662578	26.197883199205325
2	19.59917827649257	19.891185259876554	33.55322132070701	26.956415142923863
3	21.30205267651416	20.178928394997573	27.888232544954448	30.63078638353382
4	14.720936127886134	15.385163740101127	32.780798558819285	37.113101573193454
5	17.40243032688726	33.43656509713746	31.93475779176631	17.22624678420897
6	37.095546510423276	32.41047667491788	15.503918576487607	14.990058238171237
7	32.49358840473114	27.98203720673968	19.154805340336704	20.369569048192478
8	28.3426321867912	31.703710514016905	19.378757308086023	20.57489999110588
9	27.42793680044158	12.982018885516675	18.574189127653288	41.015855186388464
10	17.82764925717434	25.166764768488033	31.78509005547333	25.220495918864298
11	38.09118839114012	20.48982289373391	19.44154913602109	21.977439579104878
12	23.054627557850928	23.371851206352602	27.24279251414815	26.33072872164832
13	32.22306721923475	17.887709557315947	23.29107126855815	26.598151954891154
14	24.60613534453726	19.06379882897405	23.44530265494508	32.88476317154361
15	26.030993513287715	25.778993418683584	21.568876306095007	26.621136761933695
16	28.119246511389306	23.51805456593192	22.42261198681005	25.940086935868724
17	25.858707394412296	24.29777250570706	23.2639225471963	26.57959755268434
18	25.277858002526997	22.706224518926323	25.08675099092833	26.92916648761835
19	27.887499696034258	24.054699843403508	23.995605571383983	24.062194889178254
20	27.72257537767536	23.292636900342206	23.48047940311454	25.504308318867896
21	26.358043999582943	23.89264029809629	23.297533663581707	26.451782038739058
22	28.278274727072073	22.418548006434413	23.289139212313994	26.014038054179522
23	24.701272458904665	23.638708147248003	23.987244431430824	27.672774962416508
24	25.918268024835584	24.091042487582374	23.43081223311391	26.559877254468127
25	26.457111849067765	23.139005117617256	24.005898767581296	26.397984265733683
26	27.06600936780788	23.533710883772493	22.64233341761096	26.757946330808668
27	27.195823560626426	23.12101700775787	22.585670871553905	27.097488560061798
28	26.142186681270346	23.900435145702023	23.758162521240127	26.1992156517875
29	26.818373055410373	23.96689121823808	24.39294293138902	24.82179279496253
30	26.934163184801513	22.872747780383833	22.844766276158126	27.34832275865653
31	25.20970305294867	22.88523952334174	24.20996388054163	27.695093543167964
32	24.865530550972473	23.575483272223728	24.137645016644	27.421341160159802
33	25.916769015680636	22.417215553852238	24.606002099279042	27.06001333118808
34	27.12197237625929	22.51748261066102	24.795343611206324	25.56520140187336
35	26.976235375083736	22.22094528849764	23.73334559189709	27.069473744521538
36	25.04617780980106	22.202723999436373	24.06156197420172	28.689536216560853
37	24.51579505946569	21.721975107787088	26.922437602078357	26.839792230668863
38	24.53011892472409	20.96833992730805	25.93552328577477	28.56601786219309
39	23.857463549926834	21.802055507975897	27.969112416692564	26.37136852540471
40	25.55890556342258	20.71327519176491	27.07017328212718	26.657645962685333
41	23.11648666897847	21.803854318961832	28.22444364275214	26.855215369307555
42	22.70176080277603	22.611653696906345	27.93683375288934	26.749751747428284
43	23.072482422452094	21.02896651979708	27.583367394152464	28.315183663598358
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	268.0
1	269.5
2	271.0
3	641.5
4	1012.0
5	1012.0
6	1376.0
7	1740.0
8	1772.0
9	1804.0
10	2392.5
11	2981.0
12	2981.0
13	4618.0
14	6255.0
15	9354.0
16	12453.0
17	11990.0
18	11527.0
19	11527.0
20	12927.0
21	14327.0
22	14911.5
23	15496.0
24	18066.5
25	20637.0
26	20637.0
27	22991.5
28	25346.0
29	30051.5
30	34757.0
31	41869.5
32	48982.0
33	48982.0
34	68111.0
35	87240.0
36	94121.0
37	101002.0
38	107027.0
39	113052.0
40	113052.0
41	119050.0
42	125048.0
43	142340.0
44	159632.0
45	161395.5
46	163159.0
47	163159.0
48	169408.0
49	175657.0
50	192100.0
51	208543.0
52	233991.5
53	259440.0
54	259440.0
55	256268.5
56	253097.0
57	230290.5
58	207484.0
59	214330.0
60	221176.0
61	221176.0
62	210522.0
63	199868.0
64	162762.0
65	125656.0
66	118263.5
67	110871.0
68	110871.0
69	100326.0
70	89781.0
71	94379.5
72	98978.0
73	83196.0
74	67414.0
75	67414.0
76	41119.0
77	14824.0
78	11747.5
79	8671.0
80	7273.5
81	5876.0
82	5876.0
83	4616.5
84	3357.0
85	2754.5
86	2152.0
87	1751.5
88	1351.0
89	1351.0
90	989.5
91	628.0
92	376.0
93	124.0
94	78.0
95	32.0
96	32.0
97	22.5
98	13.0
99	7.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3001983.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.47700812064843
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.97436911360347	40.81805820574178
2	8.231718835724507	7.816347640211742
3	2.173822679241371	3.096197909855769
4	1.006123949660898	1.9107101971371732
5	0.5390339172814753	1.279585883403877
6	0.3468917222210368	0.9881628667724329
7	0.24914022978458958	0.8279902898864223
8	0.18900130903427254	0.7178573347066672
9	0.15511192645609653	0.6627825172768965
>10	0.9095091386276353	8.435209040588381
>50	0.10789487256854867	3.552830003956771
>100	0.08909433153053123	8.75386262019029
>500	0.012909474540157251	4.183885081709875
>1k	0.014461433228043325	14.36511197520201
>5k	9.170664973881132E-4	2.5914084333599443
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	7397	0.246403793758992	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	6616	0.22038765709199554	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	6259	0.20849551779607012	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	6251	0.2082290272796348	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	6137	0.20443153742043177	No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC	6060	0.20186656619974197	No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG	5885	0.19603708615271973	No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA	5763	0.19197310577708135	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	5753	0.19163999263153722	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5707	0.19010767216203422	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	5386	0.1794147401900677	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	5130	0.170887043664138	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	5030	0.1675559122086967	No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA	4948	0.16482438441523486	No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG	4835	0.1610602058705862	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	4829	0.16086033798325974	No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC	4763	0.1586617912226685	No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG	4762	0.15862847990811407	No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT	4716	0.15709615943861108	No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG	4711	0.156929602865839	No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG	4687	0.1561301313165331	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	4648	0.15483099004891102	No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG	4647	0.1547976787343566	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4558	0.15183297173901386	No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG	4522	0.150633764415055	No Hit
GTCCCTGGCTGCTGCCGCGGGGAAGCAGGAACTGGCCAAGTAC	4278	0.14250580366377824	No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA	4225	0.14074030399239437	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4160	0.13857506854635754	No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA	4132	0.13764235173883396	No Hit
GCCAGGGACTTCTGCAGAAACTGACGGAGTCTGGGGTCCGAGG	3991	0.13294545638666175	No Hit
GAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGGCGCCGA	3846	0.1281153157762719	No Hit
CTCCAGCTCGGCTTTCGCGGCGCCGAGATGCTGTCCTGCCGCC	3755	0.12508398615182031	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	3680	0.12258563756023935	No Hit
CGGCAGGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAG	3617	0.12048702474331133	No Hit
ATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCA	3610	0.12025384554143045	No Hit
GTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAGCCAGGGAC	3501	0.11662291225499945	No Hit
GTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCATCGTC	3487	0.11615655385123766	No Hit
CCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGGCTGCTGCC	3473	0.11569019544747589	No Hit
ACGCAAAGCTGGCTGCAAGAATTTCTTCTGGAAGACTTTCACA	3409	0.11355827131599346	No Hit
GCCATAGCCGGGTTTGAGTTAGCAGATCTCTGCAGCTCAAGCC	3272	0.10899462122203889	No Hit
GTTCGGAGCCATCGCTGCTGCCTGCTGATCCGCGCCTAGAGTT	3225	0.1074289894379815	No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC	3213	0.10702925366332854	No Hit
GCTTTGCGTTCTCGGGGTGCCATAGCCGGGTTTGAGTTAGCAG	3190	0.10626309342857704	No Hit
CTCTCCAGCTCGGCTTTCGCGGCGCCGAGATGCTGTCCTGCCG	3188	0.10619647079946823	No Hit
CTGCAGCTCAAGCCTCATTTCATCCTGCTCAGCAGCCTGGGAC	3183	0.10602991422669615	No Hit
TAGCAGATCTCTGCAGCTCAAGCCTCATTTCATCCTGCTCAGC	3155	0.10509719741917259	No Hit
AGTCTGGGGTCCGAGGGAGCGCCGGTGACACAGCCCAGGGCCA	3137	0.10449759375719317	No Hit
CGCCTAGAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGG	3029	0.10089997178531658	No Hit
GATCTGCTAACTCAAACCCGGCTATGGCACCCCGAGAACGCAA	3020	0.10060016995432687	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.3311314554412865E-5	0.0
10	0.0	0.0	0.0	1.998678873264772E-4	0.0
11	0.0	0.0	0.0	1.998678873264772E-4	0.0
12	0.0	0.0	0.0	2.9980183098971577E-4	0.0
13	0.0	3.3311314554412865E-5	0.0	2.9980183098971577E-4	0.0
14	0.0	3.3311314554412865E-5	0.0	3.6642446009854153E-4	0.0
15	0.0	3.3311314554412865E-5	0.0	3.997357746529544E-4	0.0
16	0.0	3.3311314554412865E-5	0.0	6.329149765338445E-4	0.0
17	0.0	3.3311314554412865E-5	0.0	9.327168075235603E-4	0.0
18	0.0	3.3311314554412865E-5	0.0	0.0010659620657412117	0.0
19	3.3311314554412865E-5	6.662262910882573E-5	0.0	0.001232518638513276	0.0
20	3.3311314554412865E-5	6.662262910882573E-5	0.0	0.0016988770422750562	0.0
21	3.3311314554412865E-5	6.662262910882573E-5	0.0	0.0021985467605912493	0.0
22	3.3311314554412865E-5	6.662262910882573E-5	0.0	0.003197886197223635	0.0
23	3.3311314554412865E-5	6.662262910882573E-5	0.0	0.005096631126825168	0.0
24	3.3311314554412865E-5	9.99339436632386E-5	0.0	0.008127960751276739	0.0
25	3.3311314554412865E-5	9.99339436632386E-5	0.0	0.010426441455531227	0.0
26	3.3311314554412865E-5	9.99339436632386E-5	0.0	0.016822213849978496	3.3311314554412865E-5
27	3.3311314554412865E-5	9.99339436632386E-5	0.0	0.048867698451323674	3.3311314554412865E-5
28	3.3311314554412865E-5	9.99339436632386E-5	0.0	0.17415155249047046	3.3311314554412865E-5
29	3.3311314554412865E-5	9.99339436632386E-5	0.0	0.34030838948788183	3.3311314554412865E-5
30	3.3311314554412865E-5	9.99339436632386E-5	0.0	0.5430743611805929	3.3311314554412865E-5
31	6.662262910882573E-5	9.99339436632386E-5	0.0	1.216862320672702	3.3311314554412865E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1940	0.0	23.268042	1
CTTATAC	4085	0.0	19.383108	37
ATACACA	1005	0.0	16.199005	37
GTACTAT	270	0.0	15.074075	1
GTATCAA	3055	0.0	14.775778	2
TATACAC	1285	0.0	14.396888	37
TATACTG	275	0.0	14.127274	5
GGACCGT	160	1.7862476E-7	13.875001	6
TCTTATA	6440	0.0	13.760094	37
GAACCGT	95	0.001245892	13.631579	6
GCGTTAT	290	0.0	13.396551	1
AAGACGG	320	0.0	13.296876	5
ATTAGAC	175	5.312886E-7	12.685715	3
CTCTTAT	9545	0.0	12.133055	37
CGTTATT	290	1.2732926E-11	12.120689	2
TTATACA	2005	0.0	11.995013	37
TACGGCT	110	0.0037474579	11.772727	4
ATCAACG	4475	0.0	11.740782	2
CAACGCA	4585	0.0	11.580153	4
TCCGTAG	160	3.7673868E-5	11.562501	34
>>END_MODULE