FastQCFastQC Report
Fri 10 Feb 2017
ERR1632193.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632193.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1691199
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41430.24497412782292327No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32190.19033833392758628No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27540.16284304803869917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14200.022.4084511
CGTATCT450.00382654320.55555527
CTTATAC22800.018.5811437
ATACCGG500.007036218418.56
CTAGACT1603.45608E-1117.343754
CTAGATA1950.017.0769233
AAACCGT650.001580350717.0769236
TCGTTTA1851.8189894E-1217.030
TATACAC8250.016.59393937
CCTAGTA803.383745E-416.18752
GTATTAT1504.6748028E-916.0333331
CGATCGC2200.015.97727319
CCCTTAC957.06282E-515.5789481
GTATCAA20900.015.3133962
GACGGAC3550.014.591557
ACGGACC3700.014.58
CCTAAAC1803.3323886E-914.3888883
CCGGTCG2450.014.34693820
TAGACTC1551.2110468E-714.3225815
AAGACGG4150.014.265065
Produced by FastQC (version 0.11.5)