##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632193.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1691199
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.81198723509179	34.0	31.0	34.0	31.0	34.0
2	32.972162353454564	34.0	31.0	34.0	31.0	34.0
3	33.05856259375744	34.0	33.0	34.0	31.0	34.0
4	36.44156423933553	37.0	37.0	37.0	35.0	37.0
5	36.310152146494886	37.0	37.0	37.0	35.0	37.0
6	36.33404643687703	37.0	37.0	37.0	35.0	37.0
7	36.31425101362998	37.0	37.0	37.0	35.0	37.0
8	36.30572629241148	37.0	37.0	37.0	35.0	37.0
9	38.100393271282684	39.0	39.0	39.0	37.0	39.0
10	38.07222982038187	39.0	38.0	39.0	35.0	39.0
11	38.13584622507464	39.0	38.0	39.0	37.0	39.0
12	38.102325036852555	39.0	38.0	39.0	37.0	39.0
13	38.12176390832776	39.0	38.0	39.0	37.0	39.0
14	39.52529477607307	40.0	39.0	41.0	37.0	41.0
15	39.53605400665445	40.0	39.0	41.0	37.0	41.0
16	39.534562756955275	41.0	39.0	41.0	37.0	41.0
17	39.522725592907754	41.0	39.0	41.0	37.0	41.0
18	39.51585827569671	41.0	39.0	41.0	37.0	41.0
19	39.53673281500285	41.0	39.0	41.0	37.0	41.0
20	39.511349640107404	41.0	39.0	41.0	37.0	41.0
21	39.48736606395818	41.0	39.0	41.0	37.0	41.0
22	39.44577367891064	40.0	39.0	41.0	37.0	41.0
23	39.39085228881994	40.0	39.0	41.0	37.0	41.0
24	39.34337472999925	40.0	39.0	41.0	36.0	41.0
25	39.291976284281155	40.0	39.0	41.0	36.0	41.0
26	39.208787375110795	40.0	39.0	41.0	36.0	41.0
27	39.12745099778323	40.0	39.0	41.0	36.0	41.0
28	39.05961037110358	40.0	39.0	41.0	35.0	41.0
29	38.98988528257171	40.0	39.0	41.0	35.0	41.0
30	38.92041504281873	40.0	38.0	41.0	35.0	41.0
31	38.84603349457988	40.0	38.0	41.0	35.0	41.0
32	38.7615845326304	40.0	38.0	41.0	35.0	41.0
33	38.67701021582913	40.0	38.0	41.0	35.0	41.0
34	38.597448910506685	40.0	38.0	41.0	35.0	41.0
35	38.572640475780794	40.0	38.0	41.0	35.0	41.0
36	38.53285331885839	40.0	38.0	41.0	35.0	41.0
37	38.47194209551922	40.0	38.0	41.0	35.0	41.0
38	38.42560337370114	40.0	38.0	41.0	35.0	41.0
39	38.368834182139416	40.0	38.0	41.0	34.0	41.0
40	38.28958035098176	40.0	38.0	41.0	34.0	41.0
41	38.24018994807826	40.0	38.0	41.0	34.0	41.0
42	38.16918115490844	40.0	37.0	41.0	34.0	41.0
43	37.30476011397831	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	0.0
13	0.0
14	1.0
15	1.0
16	2.0
17	7.0
18	21.0
19	43.0
20	100.0
21	190.0
22	333.0
23	620.0
24	1009.0
25	1526.0
26	2362.0
27	3473.0
28	5170.0
29	7345.0
30	10268.0
31	14069.0
32	18937.0
33	26528.0
34	40162.0
35	60267.0
36	98231.0
37	182945.0
38	423086.0
39	794500.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.156423342256	17.596569061358245	12.17237001677508	27.074637579610677
2	19.56724193900304	19.673497914792996	33.2215783003656	27.537681845838367
3	20.306185138472763	20.95359564427368	28.729676401180466	30.010542816073094
4	15.487532809562921	15.080839096995682	32.58226855621367	36.849359537227734
5	16.990785827096634	33.82091640309626	32.19307721918	16.9952205506271
6	36.25392399120388	32.9174745254698	15.135297501949799	15.693303981376527
7	31.035555248081394	28.683910054346057	20.1249527701944	20.15558192737815
8	28.470570287707126	31.61224669598315	19.251489623633883	20.665693392675845
9	27.50285448371244	13.785367659276051	18.8002121571737	39.91156569983781
10	18.373473494248753	25.15032234527102	31.207563391416386	25.268640769063843
11	38.11686265188189	20.256279716343258	20.324692718006574	21.30216491376828
12	23.41587240768236	23.940707155101205	27.452712542994647	25.19070789422179
13	31.867036345220168	18.167702322435147	23.03898003723985	26.926281295104832
14	24.042883185243134	19.319666106708908	23.515328474058936	33.12212223398902
15	26.77177552730341	25.839300992964166	20.80600804517978	26.582915434552646
16	26.597638716673792	24.306956189070593	22.634710640202602	26.460694454053012
17	25.417410961099197	24.653337661623496	23.936863728041466	25.992387649235837
18	25.757938598591885	23.371761690965993	24.88281982191333	25.987479888528792
19	26.19005805940046	23.86253776167086	24.61182864937834	25.335575529550336
20	26.371881724149553	23.53395431288689	24.170011926449813	25.924152036513743
21	27.206023655406607	23.59006834795905	23.75350269246848	25.450405304165862
22	27.138497598449383	23.38624845449885	23.60443685219776	25.870817094854004
23	26.170545275866413	23.400557829090484	23.920130037919844	26.508766857123263
24	25.942541356753402	23.858635204964052	23.905820663328207	26.29300277495434
25	26.17208264669031	22.98109211275551	24.075345361486143	26.771479879068043
26	25.840306196964402	23.88778612097098	24.287443405536546	25.984464276528072
27	26.135008357975615	23.441061637335405	24.01030274970598	26.413627254983002
28	25.643877509388307	23.748417542820214	24.462526290519328	26.14517865727215
29	25.361237796379964	24.023429531356154	24.62300415267511	25.992328519588764
30	25.38287924720864	24.127438580557346	24.459333289577394	26.03034888265662
31	25.927522426396894	23.763495602823795	23.87850276638054	26.43047920439877
32	24.972637755817026	23.706080715516034	24.366144965790543	26.955136562876397
33	25.02662312359456	23.217315052811642	25.06878256195752	26.68727926163627
34	25.763437655769668	22.853904241901752	25.08457017772598	26.298087924602605
35	25.48458224017398	22.784663425179414	25.67036759127696	26.060386743369644
36	24.720568070345358	22.868331875787533	25.621112595265256	26.789987458601853
37	24.532181014771176	21.925509653210533	26.453184988874757	27.089124343143535
38	23.846631886608257	21.57297869736205	27.61289475691506	26.967494659114628
39	23.739666355053426	21.01012358687535	28.324933966966633	26.925276091104593
40	23.48854274393492	21.288505965294444	28.90422711933959	26.31872417143104
41	22.574161881599977	21.26822449634845	29.772427727310625	26.38518589474095
42	22.30665935824229	21.4956962486378	29.91859621487477	26.27904817824514
43	21.38541945684689	21.04282228170665	30.298563326965073	27.273194934481392
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	114.0
1	129.0
2	144.0
3	356.5
4	569.0
5	569.0
6	750.5
7	932.0
8	906.0
9	880.0
10	1314.0
11	1748.0
12	1748.0
13	2903.5
14	4059.0
15	6274.0
16	8489.0
17	7951.5
18	7414.0
19	7414.0
20	8413.5
21	9413.0
22	8618.5
23	7824.0
24	9421.5
25	11019.0
26	11019.0
27	13267.5
28	15516.0
29	18937.0
30	22358.0
31	26324.5
32	30291.0
33	30291.0
34	36588.5
35	42886.0
36	49543.0
37	56200.0
38	63452.0
39	70704.0
40	70704.0
41	77314.0
42	83924.0
43	89319.5
44	94715.0
45	101202.0
46	107689.0
47	107689.0
48	113125.5
49	118562.0
50	122244.5
51	125927.0
52	128470.0
53	131013.0
54	131013.0
55	130524.0
56	130035.0
57	127484.5
58	124934.0
59	120592.5
60	116251.0
61	116251.0
62	106908.5
63	97566.0
64	87999.0
65	78432.0
66	69309.5
67	60187.0
68	60187.0
69	52080.0
70	43973.0
71	38195.5
72	32418.0
73	26302.5
74	20187.0
75	20187.0
76	16534.5
77	12882.0
78	10784.0
79	8686.0
80	7270.0
81	5854.0
82	5854.0
83	4503.5
84	3153.0
85	2650.5
86	2148.0
87	1760.0
88	1372.0
89	1372.0
90	989.5
91	607.0
92	343.0
93	79.0
94	55.0
95	31.0
96	31.0
97	22.0
98	13.0
99	7.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1691199.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.97283298407984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.43184691538983	59.4299032786943
2	7.572549549054802	10.294552915231424
3	2.0323981187154203	4.144435736418041
4	0.8894094647633991	2.4182272401128952
5	0.5075216278121287	1.7248841421541081
6	0.3116109033528488	1.2708645533772862
7	0.21982660604601287	1.0459566024755924
8	0.15705810010706306	0.8540547205899434
9	0.11635841129905267	0.7118289771771038
>10	0.6601870530931568	8.352734345624215
>50	0.056699558848405766	2.6279096033504383
>100	0.04210325085906227	5.568426798430011
>500	0.002083234850438443	0.8876761542445828
>1k	3.472058084064072E-4	0.6685449321199979
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4143	0.24497412782292327	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3219	0.19033833392758628	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2754	0.16284304803869917	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.9129647072875515E-5	0.0	0.0	0.0
3	0.0	5.9129647072875515E-5	0.0	0.0	0.0
4	0.0	5.9129647072875515E-5	0.0	0.0	0.0
5	0.0	2.956482353643776E-4	0.0	0.0	0.0
6	0.0	2.956482353643776E-4	0.0	0.0	0.0
7	0.0	2.956482353643776E-4	0.0	0.0	0.0
8	0.0	2.956482353643776E-4	0.0	0.0	0.0
9	0.0	2.956482353643776E-4	0.0	0.0	0.0
10	0.0	2.956482353643776E-4	0.0	1.7738894121862656E-4	0.0
11	0.0	3.547778824372531E-4	0.0	2.3651858829150206E-4	0.0
12	0.0	3.547778824372531E-4	0.0	3.547778824372531E-4	0.0
13	0.0	3.547778824372531E-4	0.0	4.139075295101286E-4	0.0
14	0.0	3.547778824372531E-4	0.0	4.139075295101286E-4	0.0
15	0.0	3.547778824372531E-4	0.0	6.504261178016307E-4	0.0
16	0.0	3.547778824372531E-4	0.0	8.278150590202572E-4	0.0
17	0.0	3.547778824372531E-4	0.0	0.0011825929414575104	0.0
18	0.0	4.730371765830041E-4	0.0	0.001478241176821888	0.0
19	0.0	4.730371765830041E-4	5.9129647072875515E-5	0.0016556301180405144	0.0
20	0.0	4.730371765830041E-4	5.9129647072875515E-5	0.0021286672946235186	0.0
21	0.0	4.730371765830041E-4	5.9129647072875515E-5	0.0026608341182793984	0.0
22	0.0	4.730371765830041E-4	5.9129647072875515E-5	0.0036069084714454064	0.0
23	0.0	5.321668236558797E-4	5.9129647072875515E-5	0.00514427929534017	0.0
24	0.0	5.321668236558797E-4	5.9129647072875515E-5	0.007686854119473817	5.9129647072875515E-5
25	0.0	5.321668236558797E-4	5.9129647072875515E-5	0.010111169649461713	5.9129647072875515E-5
26	0.0	5.321668236558797E-4	5.9129647072875515E-5	0.014959800709437506	5.9129647072875515E-5
27	0.0	5.321668236558797E-4	5.9129647072875515E-5	0.049728033188288306	5.9129647072875515E-5
28	0.0	5.912964707287552E-4	5.9129647072875515E-5	0.18052281251348895	5.9129647072875515E-5
29	0.0	7.095557648745062E-4	5.9129647072875515E-5	0.35329964126043123	5.9129647072875515E-5
30	0.0	7.095557648745062E-4	5.9129647072875515E-5	0.570955872135686	5.9129647072875515E-5
31	0.0	7.095557648745062E-4	5.9129647072875515E-5	1.2480494607671835	5.9129647072875515E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1420	0.0	22.408451	1
CGTATCT	45	0.003826543	20.555555	27
CTTATAC	2280	0.0	18.58114	37
ATACCGG	50	0.0070362184	18.5	6
CTAGACT	160	3.45608E-11	17.34375	4
CTAGATA	195	0.0	17.076923	3
AAACCGT	65	0.0015803507	17.076923	6
TCGTTTA	185	1.8189894E-12	17.0	30
TATACAC	825	0.0	16.593939	37
CCTAGTA	80	3.383745E-4	16.1875	2
GTATTAT	150	4.6748028E-9	16.033333	1
CGATCGC	220	0.0	15.977273	19
CCCTTAC	95	7.06282E-5	15.578948	1
GTATCAA	2090	0.0	15.313396	2
GACGGAC	355	0.0	14.59155	7
ACGGACC	370	0.0	14.5	8
CCTAAAC	180	3.3323886E-9	14.388888	3
CCGGTCG	245	0.0	14.346938	20
TAGACTC	155	1.2110468E-7	14.322581	5
AAGACGG	415	0.0	14.26506	5
>>END_MODULE