FastQCFastQC Report
Fri 10 Feb 2017
ERR1632192.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632192.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547225
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACA601.3352692E-624.6666664
CTTAGGA751.3704266E-824.6666662
AGTACAG502.6995956E-422.25
TAATACT450.003823284820.5555554
TAGTACA555.1383616E-420.1818184
CTTATAC2250.019.73333437
GGTATCA2750.019.5090921
TTCTATA500.00703028718.52
GACTTAC500.00703028718.57
TGCGTAC609.228389E-418.516
TTAGGAC953.5994053E-617.5263163
TAAGCTG752.0647727E-417.2666665
TGCACCG650.001578503817.0769235
TACTTAG700.00259016715.85714220
TAGAACA1659.695214E-1015.696974
AGCGAAA957.0488764E-515.57894817
TCTTATA4050.015.53086437
GAGCGAA855.3577765E-415.23529316
AAGGCTA855.3577765E-415.2352935
GCGCAAG1353.9607949E-715.0740751
Produced by FastQC (version 0.11.5)