##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632192.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	547225
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.106754991091414	33.0	31.0	34.0	30.0	34.0
2	32.31001142126182	34.0	31.0	34.0	31.0	34.0
3	32.39081730549591	34.0	31.0	34.0	30.0	34.0
4	36.01335648042396	37.0	35.0	37.0	35.0	37.0
5	35.641668417926816	37.0	35.0	37.0	33.0	37.0
6	35.62694321348623	37.0	35.0	37.0	33.0	37.0
7	35.612378820412076	37.0	35.0	37.0	33.0	37.0
8	35.57624925761798	37.0	35.0	37.0	33.0	37.0
9	37.280341724153686	39.0	37.0	39.0	34.0	39.0
10	37.13975055964183	39.0	37.0	39.0	34.0	39.0
11	37.23084288912239	39.0	37.0	39.0	34.0	39.0
12	37.1813440540911	39.0	37.0	39.0	34.0	39.0
13	37.228445337840924	39.0	37.0	39.0	34.0	39.0
14	38.43744894695966	40.0	38.0	41.0	34.0	41.0
15	38.41586550322079	40.0	38.0	41.0	34.0	41.0
16	38.38970989994974	40.0	38.0	41.0	34.0	41.0
17	38.35694275663575	40.0	38.0	41.0	34.0	41.0
18	38.33231851614966	40.0	38.0	41.0	34.0	41.0
19	38.36009319749646	40.0	38.0	41.0	34.0	41.0
20	38.33381150349491	40.0	38.0	41.0	34.0	41.0
21	38.295852711407555	40.0	38.0	41.0	34.0	41.0
22	38.24698250262689	40.0	38.0	41.0	34.0	41.0
23	38.19507880670656	40.0	38.0	41.0	34.0	41.0
24	38.127499657362144	40.0	38.0	41.0	34.0	41.0
25	38.05187811229385	40.0	38.0	41.0	33.0	41.0
26	37.919619900406595	40.0	37.0	41.0	33.0	41.0
27	37.817062451459634	40.0	37.0	41.0	33.0	41.0
28	37.732599936040934	39.0	37.0	41.0	33.0	41.0
29	37.656428343003334	39.0	37.0	40.0	33.0	41.0
30	37.56910228882087	39.0	37.0	40.0	33.0	41.0
31	37.476988441683034	39.0	37.0	40.0	32.0	41.0
32	37.369383708712135	39.0	37.0	40.0	32.0	41.0
33	37.28459226095299	39.0	36.0	40.0	32.0	41.0
34	37.20620037461739	39.0	36.0	40.0	31.0	41.0
35	37.15749828681073	39.0	36.0	40.0	31.0	41.0
36	37.105046370322995	39.0	36.0	40.0	31.0	41.0
37	37.0401699483759	39.0	36.0	40.0	31.0	41.0
38	36.975878295034036	39.0	36.0	40.0	31.0	41.0
39	36.905119466398645	39.0	36.0	40.0	31.0	41.0
40	36.83265567179862	39.0	35.0	40.0	31.0	41.0
41	36.78024761295628	39.0	35.0	40.0	31.0	41.0
42	36.736614737996256	39.0	35.0	40.0	31.0	41.0
43	35.755159212389785	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	3.0
15	1.0
16	6.0
17	15.0
18	33.0
19	52.0
20	102.0
21	204.0
22	359.0
23	539.0
24	819.0
25	1242.0
26	1859.0
27	2708.0
28	3897.0
29	5336.0
30	7202.0
31	9723.0
32	13032.0
33	17790.0
34	25329.0
35	37933.0
36	59563.0
37	100933.0
38	163164.0
39	95379.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.12376079309242	18.008131938416554	12.057014939010461	26.811092329480562
2	20.659143862213895	20.274110283704143	31.987573667138747	27.07917218694321
3	21.0120151674357	21.424094293937596	28.763853990588878	28.800036548037827
4	16.35762255013934	15.470053451505322	32.261501210653755	35.91082278770158
5	16.883000593905614	34.10534971903696	32.67157019507515	16.340079491982273
6	36.638128740463245	32.76403673077802	14.912878614829367	15.684955913929372
7	30.888025949106858	28.34592717803463	20.302252272831105	20.46379460002741
8	28.066334688656404	31.279455434236375	19.606194892411715	21.04801498469551
9	27.17748640869843	13.685047284023938	18.53350998218283	40.6039563250948
10	18.097126410525835	25.429393759422542	31.920690757914933	24.55278907213669
11	37.70715884690941	20.523916122253187	20.02759376855955	21.741331262277857
12	22.99145689615789	23.862944858147927	27.351089588377725	25.794508657316463
13	31.72424505459363	18.368130111014665	23.16597377678286	26.741651057608845
14	24.328201379688426	19.602357348439856	22.84362008314679	33.22582118872493
15	26.870848371328066	25.937776965599156	20.392891406642605	26.798483256430174
16	26.91543697747727	24.417744072365117	22.2336333318105	26.433185618347117
17	25.69528073461556	24.681986385855907	23.4532413541048	26.169491525423727
18	25.541961715930377	23.671433139933303	24.53780437662753	26.248800767508797
19	26.553976883366076	23.758782950340354	24.225318653204805	25.461921513088765
20	26.703092877701128	23.337018593814243	23.768468180364565	26.191420348120058
21	26.542646991639636	23.79624468911325	23.38306912147654	26.27803919777057
22	26.909406551235783	23.527068390515783	23.20836950066243	26.355155557586002
23	26.223217141029743	23.55064187491434	23.510987253871807	26.71515373018411
24	26.46516515144593	23.823655717483668	23.432957193110695	26.278221937959707
25	26.784229521677556	23.228288181278266	23.45104847183517	26.53643382520901
26	26.490566037735846	23.99013202978665	23.476449358125084	26.042852574352416
27	26.66087989401069	23.311800447713463	23.29517109050208	26.732148567773766
28	26.166384942208413	23.652062679884875	23.894010690301066	26.287541687605646
29	25.863036228242496	23.762072273744803	23.956325094796473	26.418566403216225
30	25.684133583078257	24.54328658230161	23.788752341358673	25.983827493261458
31	26.44890127461282	23.76079309242085	23.531819635433322	26.25848599753301
32	25.631869888985335	23.681666590524923	23.864772260039288	26.821691260450454
33	25.554753529169904	23.54424596829458	24.11713646123624	26.78386404129928
34	26.469550915985195	23.17803462926584	23.865320480606698	26.48709397414226
35	26.073187445749006	23.407190826442505	24.55553017497373	25.964091552834756
36	25.607565443830232	23.476266617935952	24.384485357942346	26.53168258029147
37	26.008131938416557	22.349490611722782	24.931061263648406	26.71131618621225
38	25.215587738133312	22.64443327698844	25.747178948330212	26.392800036548035
39	25.174105715199413	22.300150760656038	26.05436520626799	26.47137831787656
40	24.72602677143771	22.248618027319658	26.74567134176984	26.279683859472797
41	24.07199963451962	22.248435287130523	27.258257572296586	26.42130750605327
42	24.54292110192334	22.122527296815754	26.945954589063	26.388597012197906
43	23.715290785325962	22.02147197222349	27.37338389145233	26.889853350998216
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	8.5
2	9.0
3	31.0
4	53.0
5	53.0
6	62.5
7	72.0
8	71.0
9	70.0
10	104.0
11	138.0
12	138.0
13	254.0
14	370.0
15	555.0
16	740.0
17	801.0
18	862.0
19	862.0
20	1165.0
21	1468.0
22	1818.5
23	2169.0
24	2659.5
25	3150.0
26	3150.0
27	3793.0
28	4436.0
29	5847.0
30	7258.0
31	8812.0
32	10366.0
33	10366.0
34	12152.0
35	13938.0
36	16122.5
37	18307.0
38	20934.0
39	23561.0
40	23561.0
41	25661.5
42	27762.0
43	28726.0
44	29690.0
45	32344.0
46	34998.0
47	34998.0
48	36367.5
49	37737.0
50	40396.0
51	43055.0
52	45873.0
53	48691.0
54	48691.0
55	46148.5
56	43606.0
57	42127.0
58	40648.0
59	38281.0
60	35914.0
61	35914.0
62	33802.5
63	31691.0
64	28286.0
65	24881.0
66	22073.5
67	19266.0
68	19266.0
69	16680.0
70	14094.0
71	12380.0
72	10666.0
73	8693.5
74	6721.0
75	6721.0
76	5483.5
77	4246.0
78	3557.5
79	2869.0
80	2291.0
81	1713.0
82	1713.0
83	1319.0
84	925.0
85	739.5
86	554.0
87	435.0
88	316.0
89	316.0
90	238.0
91	160.0
92	98.0
93	36.0
94	22.5
95	9.0
96	9.0
97	5.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	547225.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.71226584188182
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.74048564042805	62.56012591745097
2	5.895778914055212	8.220162140031565
3	1.5932911402219776	3.3321580659200842
4	0.7155321760955392	1.9952547711356967
5	0.42509471130695653	1.4817157761304278
6	0.2579702885003966	1.0790215998747958
7	0.19207246815728082	0.9372864872760718
8	0.1373397125241442	0.7659410040104612
9	0.11014791575491296	0.6910794706532124
>10	0.8044489944462142	11.168914778761469
>50	0.09528790848334602	4.540849610380411
>100	0.03255013002603356	3.227490378374933
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	1.8274018913609576E-4	1.8274018913609576E-4	0.0	0.0	0.0
6	1.8274018913609576E-4	1.8274018913609576E-4	0.0	0.0	0.0
7	1.8274018913609576E-4	1.8274018913609576E-4	0.0	0.0	0.0
8	1.8274018913609576E-4	1.8274018913609576E-4	0.0	0.0	0.0
9	1.8274018913609576E-4	1.8274018913609576E-4	0.0	0.0	0.0
10	1.8274018913609576E-4	1.8274018913609576E-4	0.0	0.0	0.0
11	1.8274018913609576E-4	1.8274018913609576E-4	0.0	0.0	0.0
12	1.8274018913609576E-4	1.8274018913609576E-4	0.0	0.0	0.0
13	1.8274018913609576E-4	1.8274018913609576E-4	0.0	0.0	0.0
14	1.8274018913609576E-4	1.8274018913609576E-4	0.0	0.0	0.0
15	1.8274018913609576E-4	1.8274018913609576E-4	0.0	0.0	0.0
16	1.8274018913609576E-4	1.8274018913609576E-4	0.0	7.30960756544383E-4	0.0
17	1.8274018913609576E-4	1.8274018913609576E-4	0.0	9.137009456804787E-4	0.0
18	1.8274018913609576E-4	3.654803782721915E-4	0.0	9.137009456804787E-4	0.0
19	1.8274018913609576E-4	3.654803782721915E-4	0.0	9.137009456804787E-4	0.0
20	1.8274018913609576E-4	3.654803782721915E-4	0.0	0.0010964411348165744	0.0
21	1.8274018913609576E-4	3.654803782721915E-4	0.0	0.0010964411348165744	0.0
22	1.8274018913609576E-4	3.654803782721915E-4	0.0	0.0025583626479053407	0.0
23	1.8274018913609576E-4	3.654803782721915E-4	0.0	0.0032893234044497235	0.0
24	1.8274018913609576E-4	3.654803782721915E-4	0.0	0.005482205674082872	0.0
25	1.8274018913609576E-4	3.654803782721915E-4	0.0	0.006944127187171639	0.0
26	1.8274018913609576E-4	3.654803782721915E-4	0.0	0.008406048700260405	0.0
27	1.8274018913609576E-4	3.654803782721915E-4	0.0	0.026314587235597788	0.0
28	1.8274018913609576E-4	3.654803782721915E-4	0.0	0.07382703641098269	0.0
29	1.8274018913609576E-4	3.654803782721915E-4	0.0	0.1368724016629357	0.0
30	1.8274018913609576E-4	3.654803782721915E-4	0.0	0.21910548677417882	0.0
31	1.8274018913609576E-4	3.654803782721915E-4	0.0	0.4151857097172096	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACA	60	1.3352692E-6	24.666666	4
CTTAGGA	75	1.3704266E-8	24.666666	2
AGTACAG	50	2.6995956E-4	22.2	5
TAATACT	45	0.0038232848	20.555555	4
TAGTACA	55	5.1383616E-4	20.181818	4
CTTATAC	225	0.0	19.733334	37
GGTATCA	275	0.0	19.509092	1
TTCTATA	50	0.007030287	18.5	2
GACTTAC	50	0.007030287	18.5	7
TGCGTAC	60	9.228389E-4	18.5	16
TTAGGAC	95	3.5994053E-6	17.526316	3
TAAGCTG	75	2.0647727E-4	17.266666	5
TGCACCG	65	0.0015785038	17.076923	5
TACTTAG	70	0.002590167	15.857142	20
TAGAACA	165	9.695214E-10	15.69697	4
AGCGAAA	95	7.0488764E-5	15.578948	17
TCTTATA	405	0.0	15.530864	37
GAGCGAA	85	5.3577765E-4	15.235293	16
AAGGCTA	85	5.3577765E-4	15.235293	5
GCGCAAG	135	3.9607949E-7	15.074075	1
>>END_MODULE