FastQCFastQC Report
Fri 10 Feb 2017
ERR1632191.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632191.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374927
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7900.21070768442923554No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6000.160031152731065No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5220.13922710287602652No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTAT200.001840354837.026
TAGGACA652.6748457E-622.769234
ATAGGAC753.7268728E-722.1999993
AACGGCT450.003821072620.55555523
TATTAGT450.003821072620.5555554
GTTCTAG1101.7444108E-920.1818181
CTCTATG656.8862864E-519.9230771
AGGACCG951.6685954E-719.4736845
GTTCTAT801.6119406E-518.514
CGCGGTT701.21641846E-418.510
GGACCGC1103.8309736E-818.56
TGTCCGA801.6119406E-518.512
CTTATAC4000.018.037537
GACCGCG953.5932917E-617.5263167
GGTTCTA752.062589E-417.26666613
GGTATCA3350.017.1194041
ACTATTC650.001577250717.0769238
TCGGTGA650.001577250717.07692313
AGTCGGT650.001577250717.07692311
TCTTATA7200.016.95833437
Produced by FastQC (version 0.11.5)