##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632191.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	374927
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5915151482822	34.0	31.0	34.0	31.0	34.0
2	32.76246842718716	34.0	31.0	34.0	31.0	34.0
3	32.845868662432956	34.0	31.0	34.0	31.0	34.0
4	36.30082656090386	37.0	37.0	37.0	35.0	37.0
5	36.14469750111355	37.0	35.0	37.0	35.0	37.0
6	36.16269300423816	37.0	36.0	37.0	35.0	37.0
7	36.14632448449965	37.0	35.0	37.0	35.0	37.0
8	36.12821429238225	37.0	35.0	37.0	35.0	37.0
9	37.89265643711976	39.0	38.0	39.0	35.0	39.0
10	37.83791244695634	39.0	38.0	39.0	35.0	39.0
11	37.90804609964073	39.0	38.0	39.0	35.0	39.0
12	37.87740280107861	39.0	38.0	39.0	35.0	39.0
13	37.89188295321489	39.0	38.0	39.0	35.0	39.0
14	39.25224643730646	40.0	39.0	41.0	36.0	41.0
15	39.246856054645306	40.0	39.0	41.0	36.0	41.0
16	39.23584591133741	40.0	39.0	41.0	36.0	41.0
17	39.23435762161701	40.0	39.0	41.0	36.0	41.0
18	39.21879725919979	40.0	39.0	41.0	36.0	41.0
19	39.24888845028499	40.0	39.0	41.0	36.0	41.0
20	39.228524486099964	40.0	39.0	41.0	36.0	41.0
21	39.1893488599114	40.0	39.0	41.0	36.0	41.0
22	39.15255503071265	40.0	39.0	41.0	36.0	41.0
23	39.10060091697851	40.0	39.0	41.0	36.0	41.0
24	39.04245893200543	40.0	39.0	41.0	36.0	41.0
25	38.996951406540475	40.0	38.0	41.0	36.0	41.0
26	38.88738341063727	40.0	38.0	41.0	35.0	41.0
27	38.81041376054539	40.0	38.0	41.0	35.0	41.0
28	38.733788177431876	40.0	38.0	41.0	35.0	41.0
29	38.67428059328883	40.0	38.0	41.0	35.0	41.0
30	38.590064199164104	40.0	38.0	41.0	35.0	41.0
31	38.50579712850768	40.0	38.0	41.0	35.0	41.0
32	38.41616634704889	40.0	38.0	41.0	34.0	41.0
33	38.345464583772305	40.0	38.0	41.0	34.0	41.0
34	38.266129139805884	40.0	38.0	41.0	34.0	41.0
35	38.22640940769803	40.0	38.0	41.0	34.0	41.0
36	38.18475863301389	40.0	38.0	41.0	34.0	41.0
37	38.134839582105315	40.0	38.0	41.0	34.0	41.0
38	38.073793031710174	40.0	38.0	41.0	34.0	41.0
39	38.02902965110542	40.0	37.0	41.0	33.0	41.0
40	37.94867267494739	40.0	37.0	41.0	33.0	41.0
41	37.897169315626776	40.0	37.0	41.0	33.0	41.0
42	37.8382325092618	40.0	37.0	41.0	33.0	41.0
43	36.92275562976259	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.0
17	1.0
18	6.0
19	23.0
20	35.0
21	61.0
22	144.0
23	224.0
24	305.0
25	474.0
26	704.0
27	1057.0
28	1549.0
29	2027.0
30	2927.0
31	4081.0
32	5566.0
33	7403.0
34	10711.0
35	15608.0
36	25238.0
37	46233.0
38	106396.0
39	144149.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.58108911868177	17.784262003003253	12.761150837363006	26.873498040951972
2	19.380039314319855	20.166592429993038	33.6108095709298	26.8425586847573
3	20.247941599298	21.1702544762047	29.45426709732828	29.12753682716902
4	15.866555356109322	15.512619789985784	32.340428936833035	36.28039591707186
5	16.456536872511183	34.32268148199516	32.89040266505213	16.330378980441527
6	36.18864472283938	32.92747654876816	15.220829654839477	15.663049073552985
7	30.551280649299727	28.94190069000099	20.252209096704156	20.254609563995125
8	27.867558217999772	32.313223640868756	19.467256292558286	20.35196184857319
9	27.172756296559065	14.059803641775598	18.72337815094672	40.04406191071862
10	17.968831265819745	25.821026493157333	31.645893733980213	24.564248507042706
11	37.21337753749396	20.65788806887741	20.55306766383856	21.575666729790065
12	22.954868547743963	24.33060302405535	27.35572524784825	25.35880318035244
13	31.213009465842685	18.3726432078778	23.808901466152076	26.605445860127436
14	24.081487862970658	19.678230695575404	23.316272234328284	32.92400920712566
15	26.55556948419292	26.29338511230186	21.028893624625596	26.12215177887962
16	26.46035094831794	24.842702712794758	22.618269689832953	26.07867664905435
17	25.364670989285916	25.236112629925294	23.784896793242417	25.614319587546376
18	24.931786721148384	23.774761486902783	25.207573741021584	26.085878050927246
19	26.114150221243072	24.072686149570448	24.981663097082897	24.831500532103583
20	26.46035094831794	23.53018054181214	24.546111643066517	25.463356866803405
21	26.575573378284307	24.3207344363036	23.53791538086081	25.565776804551287
22	26.64518692972232	23.68914482019166	23.51924507970885	26.146423170377165
23	25.712205309300213	23.999605256489932	23.910521248136305	26.377668186073556
24	26.157358632480452	24.062017406055045	23.937193106924816	25.843430854539683
25	26.124285527582703	23.415758267609426	24.167637966857548	26.29231823795032
26	26.03626839358062	24.002005723780893	24.107359565995516	25.854366316642974
27	26.114150221243072	23.506175868902478	23.948128569028103	26.431545340826347
28	25.173167043184407	24.101758475649927	24.54904554753325	26.176028933632413
29	25.254249493901483	24.384213460220256	24.68533874594254	25.67619829993572
30	25.037407281950884	24.89818017907486	24.494368237016808	25.570044301957445
31	25.79728853883556	24.184174519306424	24.28419398976334	25.734342952094675
32	24.781090718993298	24.010274000005335	24.53730992966631	26.671325351335064
33	24.847236928788803	23.626465952038664	25.041408060769164	26.484889058403372
34	25.66366252630512	23.18664700061612	24.921117977632978	26.22857249544578
35	25.31799523640602	23.269063044272617	25.562842900084547	25.850098819236813
36	24.611724415686254	23.329341445134652	25.723407489991384	26.335526649187706
37	25.24118028309511	21.954673843174806	26.00319528868286	26.80095058504722
38	24.077220365564497	22.16698183913135	27.409869121188922	26.34592867411523
39	24.05508272277003	21.526057072443436	28.007318758051568	26.411541446734965
40	23.354946429571623	21.63034404030651	28.885089630781458	26.129619899340405
41	22.56519269084382	21.849320000960187	29.53134876922708	26.054138538968918
42	22.845247208123183	21.497251464951844	29.365449807562538	26.292051519362435
43	21.52632379103132	21.51165426869764	29.995972549322936	26.966049390948104
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15.0
1	15.0
2	15.0
3	52.5
4	90.0
5	90.0
6	106.0
7	122.0
8	129.0
9	136.0
10	211.5
11	287.0
12	287.0
13	523.5
14	760.0
15	1250.5
16	1741.0
17	1613.5
18	1486.0
19	1486.0
20	1695.0
21	1904.0
22	1938.0
23	1972.0
24	2251.5
25	2531.0
26	2531.0
27	2993.5
28	3456.0
29	4509.0
30	5562.0
31	6383.0
32	7204.0
33	7204.0
34	8479.5
35	9755.0
36	11006.0
37	12257.0
38	14275.5
39	16294.0
40	16294.0
41	18061.5
42	19829.0
43	20291.0
44	20753.0
45	22603.5
46	24454.0
47	24454.0
48	25819.5
49	27185.0
50	28768.5
51	30352.0
52	32175.0
53	33998.0
54	33998.0
55	31463.5
56	28929.0
57	27667.5
58	26406.0
59	24658.0
60	22910.0
61	22910.0
62	21470.5
63	20031.0
64	17906.5
65	15782.0
66	13981.0
67	12180.0
68	12180.0
69	10513.0
70	8846.0
71	7871.0
72	6896.0
73	5498.0
74	4100.0
75	4100.0
76	3319.0
77	2538.0
78	2127.5
79	1717.0
80	1381.5
81	1046.0
82	1046.0
83	839.5
84	633.0
85	498.5
86	364.0
87	305.0
88	246.0
89	246.0
90	180.5
91	115.0
92	69.0
93	23.0
94	13.5
95	4.0
96	4.0
97	3.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	374927.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.27368400980119
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.57744215338073	60.26204537832779
2	5.54632179389387	7.462429995581796
3	1.620808337660869	3.2711324384474536
4	0.7985830021056071	2.14894482157004
5	0.46633997764010504	1.5686204148449086
6	0.3475721114965531	1.4029473839663098
7	0.24010226336337698	1.1306794656882118
8	0.1707995417695535	0.9192251521619023
9	0.13101852041970946	0.7932688687932496
>10	0.9683966278229558	13.07195029368263
>50	0.1008514380047583	4.593794188464547
>100	0.030573073725347576	2.8642432307405477
>500	0.0011911587165719835	0.5107183677306657
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	790	0.21070768442923554	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	600	0.160031152731065	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	522	0.13922710287602652	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	2.667185878851083E-4	0.0	0.0
11	0.0	0.0	2.667185878851083E-4	0.0	0.0
12	0.0	0.0	2.667185878851083E-4	2.667185878851083E-4	0.0
13	0.0	0.0	2.667185878851083E-4	2.667185878851083E-4	0.0
14	0.0	0.0	2.667185878851083E-4	2.667185878851083E-4	0.0
15	0.0	0.0	2.667185878851083E-4	5.334371757702166E-4	0.0
16	0.0	0.0	2.667185878851083E-4	0.0016003115273106499	0.0
17	0.0	0.0	2.667185878851083E-4	0.0024004672909659747	0.0
18	0.0	0.0	2.667185878851083E-4	0.002933904466736191	0.0
19	0.0	0.0	2.667185878851083E-4	0.0032006230546212998	0.0
20	0.0	0.0	2.667185878851083E-4	0.003467341642506408	0.0
21	0.0	0.0	2.667185878851083E-4	0.004267497406161733	0.0
22	0.0	0.0	2.667185878851083E-4	0.007201401872897924	0.0
23	0.0	0.0	2.667185878851083E-4	0.008001557636553249	0.0
24	0.0	0.0	2.667185878851083E-4	0.011468899279059657	0.0
25	0.0	0.0	2.667185878851083E-4	0.013069210806370307	0.0
26	0.0	0.0	2.667185878851083E-4	0.017603426800417148	0.0
27	0.0	0.0	2.667185878851083E-4	0.03894091383122581	0.0
28	0.0	0.0	2.667185878851083E-4	0.12722476642119665	0.0
29	0.0	0.0	2.667185878851083E-4	0.243780789326989	0.0
30	0.0	0.0	2.667185878851083E-4	0.41608099710076896	0.0
31	0.0	0.0	2.667185878851083E-4	0.9143113192701513	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTTAT	20	0.0018403548	37.0	26
TAGGACA	65	2.6748457E-6	22.76923	4
ATAGGAC	75	3.7268728E-7	22.199999	3
AACGGCT	45	0.0038210726	20.555555	23
TATTAGT	45	0.0038210726	20.555555	4
GTTCTAG	110	1.7444108E-9	20.181818	1
CTCTATG	65	6.8862864E-5	19.923077	1
AGGACCG	95	1.6685954E-7	19.473684	5
GTTCTAT	80	1.6119406E-5	18.5	14
CGCGGTT	70	1.21641846E-4	18.5	10
GGACCGC	110	3.8309736E-8	18.5	6
TGTCCGA	80	1.6119406E-5	18.5	12
CTTATAC	400	0.0	18.0375	37
GACCGCG	95	3.5932917E-6	17.526316	7
GGTTCTA	75	2.062589E-4	17.266666	13
GGTATCA	335	0.0	17.119404	1
ACTATTC	65	0.0015772507	17.076923	8
TCGGTGA	65	0.0015772507	17.076923	13
AGTCGGT	65	0.0015772507	17.076923	11
TCTTATA	720	0.0	16.958334	37
>>END_MODULE