FastQCFastQC Report
Fri 10 Feb 2017
ERR1632189.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632189.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643872
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16020.24880721634113612No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15370.23871204214502262No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10700.1661820983052532No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7370.11446374434670246No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTTAA250.00549457129.5999981
GGTATCA5750.022.1999991
TAAGGCG450.00382400820.5555555
GTCCTAA609.2308E-418.51
GGATAGA500.007031604618.4999981
GTAAGAC752.0654878E-417.2666663
CTTATAC7400.017.037
AGCGTCA1107.794497E-716.8181823
CCAGTAC904.4410714E-516.4444453
TCTACAC803.379675E-416.18753
GTATCAA7950.016.0566042
TAATACT1059.330257E-615.8571424
CCGATAA700.002590833515.8571429
CGATAAC700.002590833515.85714210
TCTTATA12150.015.68312837
CTCTATG1101.4498182E-515.1363641
CGCGGTT750.004101814.810
AGGACCG1252.9536714E-614.7999995
GCGTCAG1252.9536714E-614.7999994
CGTCAGA1152.2067503E-514.4782615
Produced by FastQC (version 0.11.5)