FastQCFastQC Report
Fri 10 Feb 2017
ERR1632188.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632188.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1780146
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22940.12886583460008338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10750.022.21
CTTATAC13700.019.71532837
TCTATCG752.06807E-417.26666631
TATAGAG1005.8814367E-616.6500025
TATAGAC803.3838704E-416.18752
CGTATTA700.002593243715.85714215
GGACCGT1201.9368545E-615.4166676
TCTTATA22900.014.8646337
AATCTAT1001.0938991E-414.835
TACTATA2006.184564E-1114.82
TAGCGTA1405.9996637E-714.5357147
GTATCAA16700.014.2904192
GCGTATA1304.4469707E-614.2307699
TACACCG1304.4469707E-614.2307695
GTCTAGA1203.302358E-513.8750011
GTATGTA1601.784847E-713.8751
TATACTG1601.784847E-713.8755
TAGTCGG950.001245543213.63157930
CGTATAT1501.3065346E-613.56666710
GTACTAT2051.4242687E-913.5365851
Produced by FastQC (version 0.11.5)