##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632188.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1780146
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.644817335207335	31.0	31.0	34.0	30.0	34.0
2	31.835510682831632	31.0	31.0	34.0	30.0	34.0
3	31.933922273791026	31.0	31.0	34.0	30.0	34.0
4	35.68296813856841	37.0	35.0	37.0	33.0	37.0
5	35.02479347199612	37.0	35.0	37.0	32.0	37.0
6	34.97212981407143	37.0	35.0	37.0	32.0	37.0
7	34.95428970432762	36.0	35.0	37.0	32.0	37.0
8	34.906941902518106	36.0	35.0	37.0	32.0	37.0
9	36.552270431751104	38.0	35.0	39.0	32.0	39.0
10	36.311130098317776	38.0	35.0	39.0	32.0	39.0
11	36.45598731789415	38.0	35.0	39.0	32.0	39.0
12	36.36672385298734	38.0	35.0	39.0	32.0	39.0
13	36.4645972858406	38.0	35.0	39.0	32.0	39.0
14	37.512177091092525	39.0	37.0	40.0	33.0	41.0
15	37.46399227928496	39.0	36.0	40.0	32.0	41.0
16	37.44255471180454	39.0	36.0	40.0	32.0	41.0
17	37.39673206579685	39.0	36.0	40.0	32.0	41.0
18	37.38223662553521	39.0	36.0	40.0	32.0	41.0
19	37.40858671142704	39.0	36.0	40.0	32.0	41.0
20	37.365621696197955	39.0	36.0	40.0	32.0	41.0
21	37.328934817705964	39.0	36.0	40.0	32.0	41.0
22	37.277025030531206	39.0	36.0	40.0	32.0	41.0
23	37.199828553388315	39.0	36.0	40.0	32.0	41.0
24	37.11357326870942	39.0	36.0	40.0	31.0	41.0
25	37.022368951760136	39.0	36.0	40.0	31.0	41.0
26	36.86707382428183	39.0	36.0	40.0	31.0	41.0
27	36.75262253770197	38.0	36.0	40.0	31.0	41.0
28	36.646496972720215	38.0	36.0	40.0	30.0	41.0
29	36.5272084424536	38.0	35.0	40.0	30.0	41.0
30	36.41777191309028	38.0	35.0	40.0	30.0	41.0
31	36.3105762111647	38.0	35.0	40.0	30.0	41.0
32	36.17869377006156	38.0	35.0	40.0	30.0	41.0
33	36.05815365705959	38.0	35.0	40.0	29.0	41.0
34	35.96868234403246	38.0	35.0	40.0	29.0	41.0
35	35.924425861698985	38.0	35.0	40.0	29.0	41.0
36	35.86597728500921	38.0	34.0	40.0	29.0	41.0
37	35.79479716832215	38.0	34.0	40.0	29.0	41.0
38	35.733584773383754	38.0	34.0	40.0	29.0	41.0
39	35.669015350426314	38.0	34.0	40.0	28.0	41.0
40	35.574645001028	38.0	34.0	40.0	28.0	41.0
41	35.511013703370395	38.0	34.0	40.0	28.0	41.0
42	35.44598757629992	38.0	34.0	40.0	28.0	41.0
43	34.41050453165077	37.0	33.0	39.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	3.0
13	1.0
14	6.0
15	25.0
16	38.0
17	90.0
18	169.0
19	354.0
20	650.0
21	1111.0
22	1947.0
23	3088.0
24	4990.0
25	7429.0
26	10652.0
27	15362.0
28	21218.0
29	29400.0
30	39689.0
31	53409.0
32	70815.0
33	95693.0
34	131160.0
35	181053.0
36	250989.0
37	331889.0
38	366648.0
39	162265.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.87693256620525	17.983581121997858	12.010475545264265	27.129010766532634
2	19.848091111627923	20.08812760301683	32.499244443995046	27.564536841360205
3	20.49511669267577	21.409536071760407	28.79151485327608	29.30383238228774
4	15.694611565568216	15.250996266598356	33.006730908588395	36.047661259245025
5	16.566225466899905	34.45430880388463	32.76961552591754	16.209850203297933
6	36.65766740480837	33.59123352803646	14.807380967628497	14.943718099526668
7	31.452364019580415	28.98734148772067	19.966957766385455	19.59333672631346
8	29.21389593887243	31.80205443823147	18.86053166425675	20.123517958639347
9	27.908665918413433	13.492657343835843	17.958920223397406	40.639756514353316
10	17.874432771244606	25.497122146161043	31.433770039086685	25.19467504350767
11	38.92326809149362	20.087565851340283	20.021054452837014	20.968111604329085
12	23.70625780132641	23.801699411171892	27.517068824691908	24.974973962809795
13	32.4641349642108	17.9870078072248	22.689431091607094	26.859426136957303
14	24.11981938560096	18.700263910937643	23.210118720599322	33.96979798286208
15	26.90981526234365	25.729462639581246	20.461243066579932	26.899479031495172
16	27.001886362129845	24.222956993415146	22.107512529871144	26.66764411458386
17	25.864114516449778	24.445691533166382	23.575201135187786	26.114992815196057
18	26.1336991460251	23.411281995971116	24.23054064104854	26.224478216955237
19	26.810946967271228	23.630421324992447	23.88461395863036	25.67401774910597
20	26.95638447632947	23.32016587403505	23.59553654587882	26.127913103756654
21	27.59313000169649	23.44948110997637	23.06108600081117	25.896302887515965
22	27.628408006983697	23.369319145732987	22.961993005068123	26.04027984221519
23	26.799318707566684	23.156415260321346	23.374094034983646	26.670171997128328
24	26.46990752443901	23.5759314123673	23.21186015079662	26.74230091239707
25	26.74319971507955	22.79700653766601	23.510037940708234	26.949755806546204
26	26.445639852012135	23.466895411949356	23.616770759252333	26.470693976786176
27	26.570573424876386	23.27814684862927	23.335333169301844	26.8159465571925
28	26.382611313903464	23.43229150867401	23.733952158980216	26.451145018442308
29	26.09999404543223	23.72282947578457	23.84961682918143	26.327559649601774
30	26.034100573773163	23.886917140504206	23.561044992938783	26.51793729278385
31	26.501758844499275	23.671541547715748	23.262586327188895	26.564113280596086
32	25.741933526800608	23.589975204280996	23.62205122501188	27.046040043906512
33	25.623291572713697	23.341119211570287	24.294018580498452	26.74157063521756
34	26.404126403115253	22.868292825419935	24.173916072052517	26.553664699412295
35	26.099937870264572	23.095409028248245	24.532594517528338	26.272058583958845
36	25.754797640193555	23.179222378389188	24.311264356968472	26.75471562444878
37	25.608517503620487	22.308956681081217	24.960143718548927	27.12238209674937
38	24.93503341860724	22.322944297827256	25.82276959305585	26.919252690509655
39	24.978737699042664	21.74933966090422	26.26200322894864	27.009919411104484
40	24.704322005049022	21.750744040095586	26.923465828083764	26.621468126771624
41	23.979830867805223	21.580701807604544	27.743904151681942	26.695563172908287
42	23.786812991743375	21.576263969359815	27.955122782063942	26.681800256832865
43	23.04642428205327	20.94227102720788	28.483562584192534	27.527742106546317
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	27.0
1	31.0
2	35.0
3	115.5
4	196.0
5	196.0
6	284.5
7	373.0
8	386.0
9	399.0
10	578.0
11	757.0
12	757.0
13	1325.5
14	1894.0
15	3213.5
16	4533.0
17	4336.5
18	4140.0
19	4140.0
20	4846.0
21	5552.0
22	5618.5
23	5685.0
24	7132.5
25	8580.0
26	8580.0
27	10872.5
28	13165.0
29	16566.0
30	19967.0
31	24440.0
32	28913.0
33	28913.0
34	35225.5
35	41538.0
36	49031.5
37	56525.0
38	63994.0
39	71463.0
40	71463.0
41	79443.0
42	87423.0
43	94813.5
44	102204.0
45	108828.0
46	115452.0
47	115452.0
48	122324.5
49	129197.0
50	133655.0
51	138113.0
52	141474.5
53	144836.0
54	144836.0
55	143829.5
56	142823.0
57	139412.0
58	136001.0
59	131185.5
60	126370.0
61	126370.0
62	117115.5
63	107861.0
64	97055.5
65	86250.0
66	76151.0
67	66052.0
68	66052.0
69	56694.0
70	47336.0
71	40624.5
72	33913.0
73	27393.5
74	20874.0
75	20874.0
76	16797.5
77	12721.0
78	10376.5
79	8032.0
80	6603.0
81	5174.0
82	5174.0
83	3992.0
84	2810.0
85	2177.0
86	1544.0
87	1223.0
88	902.0
89	902.0
90	653.5
91	405.0
92	241.0
93	77.0
94	51.0
95	25.0
96	25.0
97	17.0
98	9.0
99	4.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1780146.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.31851862095778
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.62207925718293	58.3127005546075
2	7.72161954506458	10.39615978256763
3	2.20431625844331	4.451739152714878
4	1.0124116895230693	2.7261622069293625
5	0.5990229958015376	2.0162670348623863
6	0.3664047948945051	1.4799496804748373
7	0.26515019639910653	1.2494662903550704
8	0.18637548573747767	1.0037217285686753
9	0.13804705159059574	0.8363810711762855
>10	0.788066681531173	9.867108125851457
>50	0.061339155972505464	2.8294986531016435
>100	0.03399769534411354	4.160981796649821
>500	9.186512618585613E-4	0.37065991092505773
>1k	2.50541253234153E-4	0.29920401121540374
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2294	0.12886583460008338	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.617516765478786E-5	0.0	0.0	0.0
3	0.0	5.617516765478786E-5	0.0	0.0	0.0
4	0.0	5.617516765478786E-5	0.0	0.0	0.0
5	0.0	5.617516765478786E-5	0.0	0.0	0.0
6	0.0	5.617516765478786E-5	0.0	0.0	0.0
7	0.0	5.617516765478786E-5	0.0	0.0	0.0
8	0.0	5.617516765478786E-5	0.0	0.0	0.0
9	0.0	5.617516765478786E-5	0.0	5.617516765478786E-5	0.0
10	0.0	5.617516765478786E-5	0.0	1.1235033530957573E-4	0.0
11	0.0	1.685255029643636E-4	0.0	1.1235033530957573E-4	0.0
12	0.0	1.685255029643636E-4	0.0	1.1235033530957573E-4	0.0
13	0.0	1.685255029643636E-4	0.0	1.1235033530957573E-4	0.0
14	0.0	1.685255029643636E-4	0.0	1.1235033530957573E-4	0.0
15	0.0	1.685255029643636E-4	0.0	1.1235033530957573E-4	0.0
16	0.0	1.685255029643636E-4	0.0	2.8087583827393934E-4	0.0
17	0.0	1.685255029643636E-4	0.0	4.494013412383029E-4	0.0
18	0.0	1.685255029643636E-4	0.0	5.617516765478787E-4	0.0
19	0.0	1.685255029643636E-4	0.0	7.302771795122423E-4	0.0
20	0.0	1.685255029643636E-4	0.0	7.864523471670301E-4	0.0
21	0.0	1.685255029643636E-4	0.0	0.0011235033530957574	0.0
22	0.0	1.685255029643636E-4	0.0	0.002190831538536727	0.0
23	0.0	1.685255029643636E-4	0.0	0.0031458093886681205	0.0
24	0.0	1.685255029643636E-4	0.0	0.004325487909418666	0.0
25	0.0	1.685255029643636E-4	0.0	0.005224290591895272	0.0
26	0.0	1.685255029643636E-4	0.0	0.007808348304015513	0.0
27	0.0	1.685255029643636E-4	0.0	0.024099146923903993	0.0
28	0.0	2.2470067061915145E-4	0.0	0.08656593335602811	0.0
29	0.0	2.2470067061915145E-4	0.0	0.17942348548939244	0.0
30	0.0	2.2470067061915145E-4	0.0	0.3040761825153667	0.0
31	0.0	2.2470067061915145E-4	0.0	0.6923027661776057	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1075	0.0	22.2	1
CTTATAC	1370	0.0	19.715328	37
TCTATCG	75	2.06807E-4	17.266666	31
TATAGAG	100	5.8814367E-6	16.650002	5
TATAGAC	80	3.3838704E-4	16.1875	2
CGTATTA	70	0.0025932437	15.857142	15
GGACCGT	120	1.9368545E-6	15.416667	6
TCTTATA	2290	0.0	14.86463	37
AATCTAT	100	1.0938991E-4	14.8	35
TACTATA	200	6.184564E-11	14.8	2
TAGCGTA	140	5.9996637E-7	14.535714	7
GTATCAA	1670	0.0	14.290419	2
GCGTATA	130	4.4469707E-6	14.230769	9
TACACCG	130	4.4469707E-6	14.230769	5
GTCTAGA	120	3.302358E-5	13.875001	1
GTATGTA	160	1.784847E-7	13.875	1
TATACTG	160	1.784847E-7	13.875	5
TAGTCGG	95	0.0012455432	13.631579	30
CGTATAT	150	1.3065346E-6	13.566667	10
GTACTAT	205	1.4242687E-9	13.536585	1
>>END_MODULE