FastQCFastQC Report
Fri 10 Feb 2017
ERR1632187.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632187.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3078873
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT50230.16314411149794097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT40360.1310869269372267No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35430.11507457436536032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18700.023.3475931
TAGACCG450.00382724520.5555575
CTTATAC36600.018.6516437
TCTTATA62900.016.02941137
GTATCAA28300.015.2314492
CGTCTAA1406.003793E-714.5357151
TCTAGCG2800.013.21428628
ATAGGAC6450.012.9069763
TATACAC12150.012.79012337
GACGGAC5350.012.4485987
CCGTTAA1205.12578E-412.33333318
CTCTTAT95150.012.171308537
TATACTG2603.092282E-1012.0961545
CGTTATT3550.011.9859162
ATACACA10700.011.92990637
TACCGTC2951.6370905E-1111.9152557
AGACGGA7650.011.8496746
AAGACGG7500.011.8399995
CGAAAGC5500.011.77272819
TGCGTAA1100.003747496811.77272710
Produced by FastQC (version 0.11.5)