FastQCFastQC Report
Fri 10 Feb 2017
ERR1632186.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632186.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1584548
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6300.024.6666681
CTAGACA2300.017.695654
CTTATAC14550.017.54639237
GATCTAG650.001580290617.0769231
CTAGAAC1950.017.0769213
TATACAC4550.016.26373537
TCTTATA26700.016.07490737
TAGAACT2100.015.8571424
TAGTACA1059.347486E-615.8571424
TCTATAC1302.5905683E-715.6538463
GTATCAA10400.015.1201932
TCTAGAC1853.0559022E-1015.03
TCTAGCG1508.106326E-814.828
TACACCG750.00410531814.85
TAGGTTG1152.2107783E-514.478265
ATAACGC2059.276846E-1114.4390253
ACGCAAT908.2764507E-414.38888911
GTTCTAG2201.8189894E-1114.2954541
GTCTTAT1304.4462286E-614.2307691
GTCTAGG1051.6562555E-414.0952381