##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632186.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1584548
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.769521024292104	34.0	31.0	34.0	31.0	34.0
2	32.94001443944898	34.0	31.0	34.0	31.0	34.0
3	33.03013540769986	34.0	33.0	34.0	31.0	34.0
4	36.42010150528731	37.0	37.0	37.0	35.0	37.0
5	36.28289139868278	37.0	37.0	37.0	35.0	37.0
6	36.30633783261851	37.0	37.0	37.0	35.0	37.0
7	36.28297407210132	37.0	37.0	37.0	35.0	37.0
8	36.268123780409304	37.0	37.0	37.0	35.0	37.0
9	38.0731003415485	39.0	38.0	39.0	37.0	39.0
10	38.032312684753	39.0	38.0	39.0	35.0	39.0
11	38.10399369410078	39.0	38.0	39.0	37.0	39.0
12	38.06282106947849	39.0	38.0	39.0	35.0	39.0
13	38.08634702135877	39.0	38.0	39.0	37.0	39.0
14	39.483142826850305	40.0	39.0	41.0	37.0	41.0
15	39.49224700040642	40.0	39.0	41.0	37.0	41.0
16	39.48986524863873	40.0	39.0	41.0	37.0	41.0
17	39.47540244915269	40.0	39.0	41.0	37.0	41.0
18	39.465600284749975	40.0	39.0	41.0	37.0	41.0
19	39.48618785925071	40.0	39.0	41.0	37.0	41.0
20	39.457883257559885	40.0	39.0	41.0	37.0	41.0
21	39.4313715961902	40.0	39.0	41.0	37.0	41.0
22	39.39308812355321	40.0	39.0	41.0	37.0	41.0
23	39.34737035419565	40.0	39.0	41.0	37.0	41.0
24	39.29987289750768	40.0	39.0	41.0	36.0	41.0
25	39.241502308544774	40.0	39.0	41.0	36.0	41.0
26	39.168535127998645	40.0	39.0	41.0	36.0	41.0
27	39.08729871231417	40.0	39.0	41.0	36.0	41.0
28	39.02590896583757	40.0	39.0	41.0	36.0	41.0
29	38.964115318690254	40.0	39.0	41.0	35.0	41.0
30	38.89693969510548	40.0	38.0	41.0	35.0	41.0
31	38.83473646743425	40.0	38.0	41.0	35.0	41.0
32	38.75086018220969	40.0	38.0	41.0	35.0	41.0
33	38.66807064222731	40.0	38.0	41.0	35.0	41.0
34	38.59935893390418	40.0	38.0	41.0	35.0	41.0
35	38.58336068077458	40.0	38.0	41.0	35.0	41.0
36	38.542504865740895	40.0	38.0	41.0	35.0	41.0
37	38.487352860247846	40.0	38.0	41.0	34.0	41.0
38	38.44488396691044	40.0	38.0	41.0	34.0	41.0
39	38.393993113493565	40.0	38.0	41.0	34.0	41.0
40	38.3304671111257	40.0	38.0	41.0	34.0	41.0
41	38.28933424547568	40.0	38.0	41.0	34.0	41.0
42	38.24133885499209	40.0	38.0	41.0	34.0	41.0
43	37.34472038713879	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	0.0
12	1.0
13	1.0
14	1.0
15	1.0
16	3.0
17	12.0
18	17.0
19	31.0
20	65.0
21	148.0
22	351.0
23	508.0
24	944.0
25	1403.0
26	2094.0
27	3129.0
28	4773.0
29	6800.0
30	9456.0
31	13216.0
32	18248.0
33	25412.0
34	38171.0
35	57817.0
36	94124.0
37	171176.0
38	415642.0
39	721000.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.26716514741113	17.253816230243576	11.800399861664019	27.678618760681278
2	20.085917245801326	19.33245316645504	32.48623582245536	28.095393765288275
3	20.584355917271044	20.24179766091024	28.293494422384175	30.88035199943454
4	15.942022583096252	14.6823573662647	32.04364904060969	37.331971010029356
5	17.43412001403555	33.427703042129366	32.23550185920527	16.902675084629813
6	37.667019238293825	32.287251632642246	14.508301420973046	15.537427708090886
7	32.04207130361466	27.844786020997788	19.639796333086785	20.47334634230077
8	28.781772467605904	31.2526979302615	18.858374754188574	21.10715484794402
9	27.701716830288508	13.372520112991213	18.11721702340352	40.80854603331676
10	18.46608622774444	24.848915905355977	31.08848706381883	25.59651080308075
11	39.07473929473894	19.72297462746474	19.596313901503773	21.605972176292546
12	24.03221612724891	23.132148726324477	26.924208039137977	25.91142710728864
13	32.818696562047975	17.659420856925763	22.19017663081207	27.331705950214193
14	24.618818742000872	18.568891570340565	22.579814559104552	34.232475128554015
15	27.551074502003093	25.183206819862825	19.999204820554507	27.26651385757958
16	27.54173429899252	23.574230632331744	21.69874311160028	27.185291957075457
17	26.304346728530785	23.93414399563787	22.97866647144801	26.782842804383332
18	26.602665239550966	22.787002981291828	23.69382309655498	26.91650868260223
19	27.081855519681326	23.110060408394066	23.308855269767783	26.499228802156832
20	27.320724900728788	22.937455981137838	23.139848082860222	26.601971035273152
21	27.889719970616227	22.94881568750205	22.761885408330958	26.399578933550767
22	28.019094404208644	22.76144364197235	22.397869928837753	26.821592024981257
23	27.14124154017423	22.73960776196114	22.761759189371354	27.357391508493272
24	27.011425340223205	23.034455251592252	22.65617702966398	27.297942378520563
25	27.258183406245816	22.36782981645239	22.91890179407629	27.455084983225504
26	27.07264153563035	22.81123702153548	22.876744661569106	27.23937678126507
27	26.962767931296494	22.813067196449712	22.839699396925813	27.38446547532798
28	26.619515470657877	22.895614396029657	23.15530990541151	27.329560227900952
29	26.400904232626594	23.21570567758124	23.337317645158116	27.046072444634056
30	26.286108088868243	23.372785172806378	23.2883446888324	27.05276204949298
31	27.083117709277342	23.069417903402105	22.66621143695237	27.18125295036818
32	26.265723726892464	22.987438689140376	23.209331620121322	27.53750596384584
33	26.07077854378662	22.754249161275013	23.776054748735916	27.39891754620245
34	26.767507200791645	22.16726788964424	23.732509207673104	27.332715701891015
35	26.24792685358853	22.300176454105525	24.445520110466834	27.00637658183911
36	26.020417178905277	22.201788775095483	24.38165331690804	27.3961407290912
37	25.82155920805176	21.530303910010932	24.985421710165927	27.662715171771385
38	25.147928620653964	21.455582285926333	26.0325341990271	27.363954894392595
39	25.089426132878273	21.094091185625174	26.561202311321587	27.255280370174965
40	24.63592141102699	21.133219063101908	27.194821488525434	27.036038037345666
41	23.97567003334705	20.815967708141375	28.03695438699238	27.1714078715192
42	23.582939740544305	21.14621961594095	28.152002968669933	27.118837674844816
43	22.816475108358976	20.641091339612306	28.64558221019496	27.896851341833763
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	19.0
2	30.0
3	66.0
4	102.0
5	102.0
6	130.5
7	159.0
8	157.0
9	155.0
10	249.5
11	344.0
12	344.0
13	588.5
14	833.0
15	1232.0
16	1631.0
17	1763.5
18	1896.0
19	1896.0
20	2432.0
21	2968.0
22	3398.5
23	3829.0
24	4967.5
25	6106.0
26	6106.0
27	7671.5
28	9237.0
29	11654.5
30	14072.0
31	17605.0
32	21138.0
33	21138.0
34	26548.0
35	31958.0
36	38300.0
37	44642.0
38	50907.0
39	57172.0
40	57172.0
41	64218.0
42	71264.0
43	78089.0
44	84914.0
45	92880.5
46	100847.0
47	100847.0
48	107488.0
49	114129.0
50	119548.0
51	124967.0
52	128714.5
53	132462.0
54	132462.0
55	132320.0
56	132178.0
57	129835.0
58	127492.0
59	124747.0
60	122002.0
61	122002.0
62	113369.5
63	104737.0
64	93829.5
65	82922.0
66	73745.5
67	64569.0
68	64569.0
69	55100.0
70	45631.0
71	38932.5
72	32234.0
73	25548.5
74	18863.0
75	18863.0
76	15241.0
77	11619.0
78	9532.5
79	7446.0
80	6172.0
81	4898.0
82	4898.0
83	3702.0
84	2506.0
85	1978.0
86	1450.0
87	1071.0
88	692.0
89	692.0
90	523.0
91	354.0
92	212.0
93	70.0
94	42.0
95	14.0
96	14.0
97	10.5
98	7.0
99	4.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1584548.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.641567984795024
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.17021676409976	54.20366140472183
2	8.031261516633979	10.222441516290587
3	2.4853008273067134	4.745053246911225
4	1.196981348847571	3.047110795568573
5	0.6964869106472593	2.21627595372387
6	0.46696557874093364	1.7831052975600166
7	0.328629288421322	1.464013824060226
8	0.2490893482123704	1.268194935083668
9	0.19072796305793258	1.0924403964797598
>10	1.076023719405034	12.574861181953597
>50	0.07166055495014101	3.153367698386574
>100	0.03576460924692439	3.8952065663393225
>500	8.915704301121249E-4	0.33426718292079466
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.262189596023598E-4	0.0
5	0.0	6.31094798011799E-5	0.0	1.262189596023598E-4	0.0
6	0.0	6.31094798011799E-5	0.0	1.262189596023598E-4	0.0
7	0.0	6.31094798011799E-5	0.0	1.262189596023598E-4	0.0
8	0.0	6.31094798011799E-5	0.0	1.262189596023598E-4	0.0
9	0.0	6.31094798011799E-5	0.0	1.262189596023598E-4	0.0
10	0.0	6.31094798011799E-5	0.0	1.262189596023598E-4	0.0
11	0.0	1.262189596023598E-4	0.0	1.262189596023598E-4	0.0
12	0.0	1.262189596023598E-4	0.0	1.262189596023598E-4	0.0
13	0.0	1.262189596023598E-4	0.0	1.8932843940353968E-4	0.0
14	0.0	1.262189596023598E-4	0.0	1.8932843940353968E-4	0.0
15	0.0	1.262189596023598E-4	0.0	3.1554739900589945E-4	0.0
16	0.0	1.262189596023598E-4	0.0	6.310947980117989E-4	0.0
17	0.0	1.262189596023598E-4	0.0	0.0010097516768188784	0.0
18	0.0	1.262189596023598E-4	0.0	0.0010728611566200581	0.0
19	0.0	1.262189596023598E-4	0.0	0.001199080116222418	0.0
20	0.0	1.262189596023598E-4	0.0	0.0015146275152283174	6.31094798011799E-5
21	0.0	1.262189596023598E-4	0.0	0.002019503353637757	6.31094798011799E-5
22	0.0	1.262189596023598E-4	0.0	0.002903036070854275	6.31094798011799E-5
23	0.0	1.262189596023598E-4	0.0	0.004102116187076693	6.31094798011799E-5
24	0.0	1.262189596023598E-4	0.0	0.00599540058111209	6.31094798011799E-5
25	0.0	1.262189596023598E-4	0.0	0.007446918616539228	6.31094798011799E-5
26	0.0	1.262189596023598E-4	0.0	0.010728611566200582	6.31094798011799E-5
27	0.0	1.262189596023598E-4	0.0	0.03067120718337343	6.31094798011799E-5
28	0.0	1.262189596023598E-4	0.0	0.10596081658618105	6.31094798011799E-5
29	0.0	1.8932843940353968E-4	0.0	0.21621307779884233	6.31094798011799E-5
30	0.0	1.8932843940353968E-4	0.0	0.3594715969475207	6.31094798011799E-5
31	0.0	1.8932843940353968E-4	0.0	0.7893102638733569	6.31094798011799E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	630	0.0	24.666668	1
CTAGACA	230	0.0	17.69565	4
CTTATAC	1455	0.0	17.546392	37
GATCTAG	65	0.0015802906	17.076923	1
CTAGAAC	195	0.0	17.076921	3
TATACAC	455	0.0	16.263735	37
TCTTATA	2670	0.0	16.074907	37
TAGAACT	210	0.0	15.857142	4
TAGTACA	105	9.347486E-6	15.857142	4
TCTATAC	130	2.5905683E-7	15.653846	3
GTATCAA	1040	0.0	15.120193	2
TCTAGAC	185	3.0559022E-10	15.0	3
TCTAGCG	150	8.106326E-8	14.8	28
TACACCG	75	0.004105318	14.8	5
TAGGTTG	115	2.2107783E-5	14.47826	5
ATAACGC	205	9.276846E-11	14.439025	3
ACGCAAT	90	8.2764507E-4	14.388889	11
GTTCTAG	220	1.8189894E-11	14.295454	1
GTCTTAT	130	4.4462286E-6	14.230769	1
GTCTAGG	105	1.6562555E-4	14.095238	1
>>END_MODULE