FastQCFastQC Report
Fri 10 Feb 2017
ERR1632185.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632185.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1505404
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41870.2781313189017699No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29770.19775422411525412No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28850.19164290781743637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13000.023.338461
ATACGCA450.003826349520.5555576
TCCTATA701.21951605E-418.52
CTTATAC16800.017.3988137
AGACCGT1151.2427445E-616.0869566
GTATAAG2055.456968E-1215.3414631
GTACACT1508.105417E-814.84
TACGATT1001.0937209E-414.87
GTATCAA20550.014.5839422
CGAACGA1405.997899E-714.53571416
GTATAGA1051.6561685E-414.0952381
TCTTATA26950.013.93506437
ATACCGT1601.7842285E-713.8750016
TACTATA1356.5697313E-613.7037032
TATAAGC1501.3061508E-613.5666672
TCTAGCG2051.4242687E-913.53658528
CGTTTAT2051.4242687E-913.53658531
CTAGCGG2051.4242687E-913.53658529
TATACAC5750.013.51304237
ATAACGA1652.596189E-713.45454512
Produced by FastQC (version 0.11.5)