##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632185.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1505404
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.6277331533595	34.0	31.0	34.0	31.0	34.0
2	32.79341957374897	34.0	31.0	34.0	31.0	34.0
3	32.88198716092159	34.0	31.0	34.0	31.0	34.0
4	36.32587398465794	37.0	37.0	37.0	35.0	37.0
5	36.17337538627505	37.0	35.0	37.0	35.0	37.0
6	36.19576671777144	37.0	36.0	37.0	35.0	37.0
7	36.17515630355705	37.0	36.0	37.0	35.0	37.0
8	36.164360530462254	37.0	36.0	37.0	35.0	37.0
9	37.927091996567036	39.0	38.0	39.0	35.0	39.0
10	37.87419456836836	39.0	38.0	39.0	35.0	39.0
11	37.948374655574185	39.0	38.0	39.0	35.0	39.0
12	37.90632082816307	39.0	38.0	39.0	35.0	39.0
13	37.933130907052195	39.0	38.0	39.0	35.0	39.0
14	39.29106870979484	40.0	39.0	41.0	36.0	41.0
15	39.29350659357887	40.0	39.0	41.0	36.0	41.0
16	39.2912467350957	40.0	39.0	41.0	36.0	41.0
17	39.27545097528637	40.0	39.0	41.0	36.0	41.0
18	39.269682424120035	40.0	39.0	41.0	36.0	41.0
19	39.294698964530454	40.0	39.0	41.0	36.0	41.0
20	39.26959075437557	40.0	39.0	41.0	36.0	41.0
21	39.24309155548942	40.0	39.0	41.0	36.0	41.0
22	39.20217496432851	40.0	39.0	41.0	36.0	41.0
23	39.14996771630738	40.0	39.0	41.0	36.0	41.0
24	39.09222640566917	40.0	39.0	41.0	36.0	41.0
25	39.04014935525613	40.0	39.0	41.0	36.0	41.0
26	38.95318798143223	40.0	38.0	41.0	35.0	41.0
27	38.86808192352352	40.0	38.0	41.0	35.0	41.0
28	38.79385600144546	40.0	38.0	41.0	35.0	41.0
29	38.715443827703396	40.0	38.0	41.0	35.0	41.0
30	38.64171876785235	40.0	38.0	41.0	35.0	41.0
31	38.56132041631349	40.0	38.0	41.0	35.0	41.0
32	38.46891332824943	40.0	38.0	41.0	34.0	41.0
33	38.378917553028955	40.0	38.0	41.0	34.0	41.0
34	38.29704717139054	40.0	38.0	41.0	34.0	41.0
35	38.25881225239205	40.0	38.0	41.0	34.0	41.0
36	38.21294881639746	40.0	38.0	41.0	34.0	41.0
37	38.151951901283645	40.0	38.0	41.0	34.0	41.0
38	38.09348786106587	40.0	38.0	41.0	34.0	41.0
39	38.0354987764082	40.0	37.0	41.0	33.0	41.0
40	37.95510241768987	40.0	37.0	41.0	33.0	41.0
41	37.891209270069695	40.0	37.0	41.0	33.0	41.0
42	37.82166780478862	40.0	37.0	41.0	33.0	41.0
43	36.93811760829651	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	2.0
12	1.0
13	0.0
14	6.0
15	1.0
16	5.0
17	10.0
18	23.0
19	42.0
20	99.0
21	229.0
22	389.0
23	674.0
24	1151.0
25	1793.0
26	2686.0
27	4047.0
28	5848.0
29	8343.0
30	11680.0
31	15748.0
32	21321.0
33	29260.0
34	42589.0
35	62481.0
36	99568.0
37	182914.0
38	418214.0
39	596277.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.33640670544253	17.70986393021408	12.069118987328318	26.884610377015072
2	19.033893891606503	19.83261636079086	33.522629141413205	27.610860606189437
3	19.85320883962046	21.25615449407601	29.20936838217515	29.681268284128382
4	14.912408894888015	15.058881203982452	33.43295221747783	36.5957576836517
5	16.320070891269054	34.480179406989755	32.891569306312455	16.308180395428735
6	35.875153779317706	33.90810705963317	15.210534846459819	15.006204314589306
7	30.96271831348927	29.29233614365313	20.217629287553372	19.527316255304225
8	28.772010702774804	32.113904307415154	19.235102337977047	19.878982651832995
9	27.435425972031428	13.73936830246233	18.558739049451177	40.26646667605507
10	17.691994972778073	25.60920523660094	31.622740473653582	25.076059316967402
11	38.152084091712254	20.308103339701503	20.457232742838467	21.08257982574777
12	23.265648291089967	24.132525222465198	27.915895002271817	24.685931484173018
13	31.814649090875275	18.183889507401336	23.308161795770438	26.69329960595295
14	23.72060921852207	19.048906472946797	23.98744788774309	33.243036420788044
15	26.50271953575253	25.97030431698069	21.115859928630453	26.411116218636327
16	26.484784150965456	24.523317328770215	22.731173824435167	26.260724695829158
17	25.2491689938382	24.88979702458609	24.1608897013692	25.700144280206512
18	25.55294127025038	23.68121779934157	24.98957090588307	25.776270024524976
19	26.024376180746167	24.00511756312591	24.81466769053357	25.15583856559435
20	26.323033551126475	23.682347064309646	24.410390831962715	25.58422855260116
21	26.973091608631307	23.78344949262789	23.9782144859453	25.2652444127955
22	27.0460952674498	23.54484244760875	23.91025930580761	25.49880297913384
23	26.108539634543288	23.5237849773217	24.202805359890103	26.16487002824491
24	25.805365204290677	23.91185356223313	24.13458447034816	26.14819676312804
25	26.04809074507574	23.207125794803254	24.355455412633418	26.389328047487588
26	25.709510536706425	23.856586006148518	24.534410696397778	25.899492760747282
27	25.877970299002794	23.604959200320977	24.237081873038733	26.279988627637497
28	25.6913094425151	23.833934279435955	24.628405398152257	25.84635087989669
29	25.24093200230636	24.12973527372054	24.819450459810124	25.809882264162976
30	25.254948173380697	24.409062284941452	24.46260272989842	25.87338681177943
31	25.80503306753536	24.121099718082323	24.030293529178877	26.04357368520344
32	24.965258495393925	23.973697426073002	24.54537120932321	26.51567286920986
33	24.80722782721449	23.640697115193	25.328549678358765	26.22352537923375
34	25.574198022590615	23.195168871611873	25.254416754572194	25.976216351225318
35	25.24671118184886	23.42175256608857	25.646072416441037	25.685463835621537
36	24.771157775587152	23.407072121503596	25.489702432038175	26.332067670871073
37	24.73661555303427	22.571548899830212	26.017799872990903	26.674035674144612
38	24.05287882854038	22.56988821605363	26.907793522536146	26.469439432869844
39	24.048494623370207	22.040130091324322	27.357838825989568	26.553536459315907
40	23.79467571495758	22.1657442121849	28.043767653068546	25.995812419788972
41	23.08303950301713	21.937765543335875	28.845280070997553	26.13391488264944
42	22.887875945593343	22.13445692983412	28.89975049886941	26.077916625703136
43	22.216228998992964	21.412059487021423	29.328074058525154	27.043637455460463
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	97.0
1	117.0
2	137.0
3	372.0
4	607.0
5	607.0
6	747.5
7	888.0
8	865.0
9	842.0
10	1245.0
11	1648.0
12	1648.0
13	2758.5
14	3869.0
15	6022.5
16	8176.0
17	7795.5
18	7415.0
19	7415.0
20	8262.0
21	9109.0
22	8703.5
23	8298.0
24	9853.5
25	11409.0
26	11409.0
27	13803.0
28	16197.0
29	19389.5
30	22582.0
31	26448.5
32	30315.0
33	30315.0
34	35831.5
35	41348.0
36	47220.0
37	53092.0
38	58663.5
39	64235.0
40	64235.0
41	69791.0
42	75347.0
43	79752.5
44	84158.0
45	89503.5
46	94849.0
47	94849.0
48	99193.0
49	103537.0
50	106082.5
51	108628.0
52	110571.0
53	112514.0
54	112514.0
55	111726.0
56	110938.0
57	109325.0
58	107712.0
59	104342.0
60	100972.0
61	100972.0
62	93612.0
63	86252.0
64	78085.0
65	69918.0
66	62172.0
67	54426.0
68	54426.0
69	47175.5
70	39925.0
71	34143.0
72	28361.0
73	23080.5
74	17800.0
75	17800.0
76	14637.0
77	11474.0
78	9416.0
79	7358.0
80	6132.5
81	4907.0
82	4907.0
83	3849.5
84	2792.0
85	2237.5
86	1683.0
87	1368.5
88	1054.0
89	1054.0
90	746.5
91	439.0
92	249.0
93	59.0
94	42.5
95	26.0
96	26.0
97	18.5
98	11.0
99	5.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1505404.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.80747425098595
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.42447854467113	59.28033077823661
2	7.4140818934246235	10.054603341661826
3	1.975083977698287	4.017763678839344
4	0.9402751280964223	2.550307261489627
5	0.5161878785746061	1.7500698142559334
6	0.34065607708880047	1.3859416905384434
7	0.2327745565567949	1.104869832500668
8	0.15369094402168273	0.833711578348798
9	0.13270173929466242	0.8098352793255486
>10	0.7740765072375463	9.75785613102452
>50	0.05977656272917522	2.822233091780797
>100	0.0345522616091179	4.39490001518162
>500	0.0012724162919451917	0.4903984661267885
>1k	3.915127052139052E-4	0.7471790406895906
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4187	0.2781313189017699	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2977	0.19775422411525412	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2885	0.19164290781743637	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	6.642735106323619E-5	0.0
11	0.0	0.0	0.0	1.3285470212647237E-4	0.0
12	0.0	0.0	0.0	1.9928205318970853E-4	0.0
13	0.0	0.0	0.0	1.9928205318970853E-4	0.0
14	0.0	0.0	0.0	2.6570940425294474E-4	0.0
15	0.0	0.0	0.0	3.9856410637941706E-4	0.0
16	0.0	0.0	0.0	7.971282127588341E-4	0.0
17	0.0	0.0	0.0	9.964102659485428E-4	0.0
18	0.0	0.0	0.0	0.0011956923191382514	0.0
19	0.0	0.0	0.0	0.0012621196702014875	0.0
20	0.0	0.0	0.0	0.001527829074454432	0.0
21	0.0	0.0	0.0	0.001859965829770613	6.642735106323619E-5
22	0.0	0.0	0.0	0.002524239340402975	6.642735106323619E-5
23	0.0	0.0	0.0	0.0035870769574147536	6.642735106323619E-5
24	0.0	0.0	0.0	0.005314188085058895	6.642735106323619E-5
25	0.0	0.0	0.0	0.006642735106323618	6.642735106323619E-5
26	0.0	0.0	0.0	0.009764820606295719	6.642735106323619E-5
27	0.0	0.0	0.0	0.03281511142523867	6.642735106323619E-5
28	0.0	0.0	0.0	0.12654410377546493	6.642735106323619E-5
29	0.0	0.0	0.0	0.269163626508233	6.642735106323619E-5
30	0.0	0.0	0.0	0.4584151496873929	6.642735106323619E-5
31	0.0	0.0	0.0	0.9999309155548942	6.642735106323619E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1300	0.0	23.33846	1
ATACGCA	45	0.0038263495	20.555557	6
TCCTATA	70	1.21951605E-4	18.5	2
CTTATAC	1680	0.0	17.39881	37
AGACCGT	115	1.2427445E-6	16.086956	6
GTATAAG	205	5.456968E-12	15.341463	1
GTACACT	150	8.105417E-8	14.8	4
TACGATT	100	1.0937209E-4	14.8	7
GTATCAA	2055	0.0	14.583942	2
CGAACGA	140	5.997899E-7	14.535714	16
GTATAGA	105	1.6561685E-4	14.095238	1
TCTTATA	2695	0.0	13.935064	37
ATACCGT	160	1.7842285E-7	13.875001	6
TACTATA	135	6.5697313E-6	13.703703	2
TATAAGC	150	1.3061508E-6	13.566667	2
TCTAGCG	205	1.4242687E-9	13.536585	28
CGTTTAT	205	1.4242687E-9	13.536585	31
CTAGCGG	205	1.4242687E-9	13.536585	29
TATACAC	575	0.0	13.513042	37
ATAACGA	165	2.596189E-7	13.454545	12
>>END_MODULE