FastQCFastQC Report
Fri 10 Feb 2017
ERR1632184.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632184.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2090220
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT80650.3858445522480887No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT70370.33666312636947304No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48770.2333247217996192No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA33030.1580216436547349No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32940.1575910669690272No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC26300.1258240759345906No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC23550.11266756609352126No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT23300.11147151974433313No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCG21680.10372113940159408No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC21290.10185530709686061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34100.023.3826981
TTTAGCG2250.018.91111226
GTACTAA953.6092442E-617.5263161
CGTAGCG650.001580519317.07692315
GTATCAA49200.016.20632
TAGCGGG2700.015.75925828
CGGTTCT2300.015.28260912
TACTTAT2550.014.50980530
CGAATTA908.278012E-414.38888815
CTTATAC16350.014.3700337
TCTTATA23250.014.16344137
GTGCAAC800.00630089613.8750011
GGACCGT800.00630089613.8750016
GACCTTA800.00630089613.8750016
GACGGTA2800.013.8758
TACGACG2950.013.796615
TTTAGGA5650.013.7522132
CTTATGC3500.013.7428573
CCGAATT950.001245670113.63157914
AATAACG2850.013.6315782