FastQCFastQC Report
Fri 10 Feb 2017
ERR1632183.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632183.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84993
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12641.4871812972833056No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11071.3024602026049203No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6980.8212441024554963No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT5760.6777028696480887Illumina PCR Primer Index 10 (95% over 22bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4040.4753332627392844No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA3320.3906204040332733No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT3210.3776781617309661No Hit
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC2970.34944054216229575No Hit
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC1970.23178379395950255No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA1850.21766498417516736No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1710.20119303942677633No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG1680.19766333698069252No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC1680.19766333698069252Illumina PCR Primer Index 10 (95% over 23bp)
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC1650.19413363453460875No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA1560.18354452719635733No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC1560.18354452719635733No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA1520.17883825726824562No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA1470.17295541985810597No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG1420.1670725824479663No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA1400.16471944748391046No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG1220.14354123280740766No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC1100.1294224230230725No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT1100.1294224230230725No Hit
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGC1040.1223630181309049No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.1188333156848211No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTC990.11648018072076523No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCG940.11059734331062558No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT940.11059734331062558No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC910.10706764086454179No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT870.10236137093643007No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT860.10118480345440214No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA850.1000082359723742No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTT250.005468089629.62
TTGGAGC250.005468089629.63
TTTGGAG250.005468089629.62
CGAAATT601.3132158E-624.66666813
GCGAAAT601.3132158E-624.66666812
GTCCTAA601.3132158E-624.6666681
AAGGTAG601.3132158E-624.6666686
TCCTAAG601.3132158E-624.6666682
GTAGCGA601.3132158E-624.6666689
AAGTTCC601.3132158E-624.66666831
AGCGAAA601.3132158E-624.66666811
AGTTCCG601.3132158E-624.66666832
TAAGGTA601.3132158E-624.6666685
CTTATAC1002.0008883E-1124.05000137
TCTCAGG400.001916581923.1250022
ATACACA802.651359E-823.1250021
GAAATTC652.6346206E-622.7692314
CGGGTAA652.6346206E-622.7692326
AGGTAGC652.6346206E-622.769237
GGGTAAG652.6346206E-622.7692327
Produced by FastQC (version 0.11.5)