FastQCFastQC Report
Fri 10 Feb 2017
ERR1632182.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632182.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68694
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT2700.39304742772294526RNA PCR Primer, Index 26 (100% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT1310.19070078900631787No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC1210.17614347686843101No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA970.14120592773750254No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC840.12228142195824963No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC740.10772410982036278No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC720.1048126473927854No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC710.10335691617899671RNA PCR Primer, Index 26 (100% over 23bp)
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC690.10044545375141935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT401.5712503E-632.37536
TGCGCGC250.00546086329.59999819
TTGCGCG250.00546086329.59999818
ATTTGCG250.00546086329.59999816
ATGCCGT453.9365696E-628.77777734
TATGCCG508.929628E-625.89999833
CCGTCTT508.929628E-625.89999837
TGCCGTC508.929628E-625.89999835
GGTATCA802.6324415E-823.1251
GGAGTGC502.666415E-422.19999923
CTTATAC450.003789828220.55555537
TTTGCGC450.003789828220.55555517
TGGAGTG555.075718E-420.18181822
ACATCTA555.075718E-420.18181824
CGGACAT609.1167947E-418.521
GAGTGCA609.1167947E-418.524
CGTATGC701.1981474E-418.531
ATCTAAG609.1167947E-418.526
TCTTATA801.5809064E-518.537
CATCTAA609.1167947E-418.525