Basic Statistics
Measure | Value |
---|---|
Filename | ERR1632182.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 68694 |
Sequences flagged as poor quality | 0 |
Sequence length | 43 |
%GC | 53 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT | 270 | 0.39304742772294526 | RNA PCR Primer, Index 26 (100% over 22bp) |
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT | 131 | 0.19070078900631787 | No Hit |
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC | 121 | 0.17614347686843101 | No Hit |
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA | 97 | 0.14120592773750254 | No Hit |
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC | 84 | 0.12228142195824963 | No Hit |
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC | 74 | 0.10772410982036278 | No Hit |
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC | 72 | 0.1048126473927854 | No Hit |
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC | 71 | 0.10335691617899671 | RNA PCR Primer, Index 26 (100% over 23bp) |
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC | 69 | 0.10044545375141935 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCCGTCT | 40 | 1.5712503E-6 | 32.375 | 36 |
TGCGCGC | 25 | 0.005460863 | 29.599998 | 19 |
TTGCGCG | 25 | 0.005460863 | 29.599998 | 18 |
ATTTGCG | 25 | 0.005460863 | 29.599998 | 16 |
ATGCCGT | 45 | 3.9365696E-6 | 28.777777 | 34 |
TATGCCG | 50 | 8.929628E-6 | 25.899998 | 33 |
CCGTCTT | 50 | 8.929628E-6 | 25.899998 | 37 |
TGCCGTC | 50 | 8.929628E-6 | 25.899998 | 35 |
GGTATCA | 80 | 2.6324415E-8 | 23.125 | 1 |
GGAGTGC | 50 | 2.666415E-4 | 22.199999 | 23 |
CTTATAC | 45 | 0.0037898282 | 20.555555 | 37 |
TTTGCGC | 45 | 0.0037898282 | 20.555555 | 17 |
TGGAGTG | 55 | 5.075718E-4 | 20.181818 | 22 |
ACATCTA | 55 | 5.075718E-4 | 20.181818 | 24 |
CGGACAT | 60 | 9.1167947E-4 | 18.5 | 21 |
GAGTGCA | 60 | 9.1167947E-4 | 18.5 | 24 |
CGTATGC | 70 | 1.1981474E-4 | 18.5 | 31 |
ATCTAAG | 60 | 9.1167947E-4 | 18.5 | 26 |
TCTTATA | 80 | 1.5809064E-5 | 18.5 | 37 |
CATCTAA | 60 | 9.1167947E-4 | 18.5 | 25 |