##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632182.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	68694
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.72891373336827	34.0	31.0	34.0	31.0	34.0
2	32.88652575188517	34.0	31.0	34.0	31.0	34.0
3	32.87199755437156	34.0	31.0	34.0	31.0	34.0
4	36.336346696945874	37.0	37.0	37.0	35.0	37.0
5	36.254520045418815	37.0	37.0	37.0	35.0	37.0
6	36.30215157073398	37.0	37.0	37.0	35.0	37.0
7	36.29683815180365	37.0	37.0	37.0	35.0	37.0
8	36.26447724692113	37.0	37.0	37.0	35.0	37.0
9	38.056482371095	39.0	38.0	39.0	35.0	39.0
10	37.992502984248986	39.0	38.0	39.0	35.0	39.0
11	38.11129065129415	39.0	38.0	39.0	37.0	39.0
12	38.0742714065275	39.0	38.0	39.0	35.0	39.0
13	38.10408478178589	39.0	38.0	39.0	37.0	39.0
14	39.46736250618686	40.0	39.0	41.0	37.0	41.0
15	39.495283430867325	40.0	39.0	41.0	37.0	41.0
16	39.38654030919731	40.0	39.0	41.0	37.0	41.0
17	39.40629458176842	40.0	39.0	41.0	37.0	41.0
18	39.438014964916874	40.0	39.0	41.0	37.0	41.0
19	39.47625702390311	40.0	39.0	41.0	37.0	41.0
20	39.4735639211576	41.0	39.0	41.0	37.0	41.0
21	39.439485253442804	40.0	39.0	41.0	37.0	41.0
22	39.39393542376336	40.0	39.0	41.0	37.0	41.0
23	39.33883599732145	40.0	39.0	41.0	36.0	41.0
24	39.283197950330454	40.0	39.0	41.0	36.0	41.0
25	39.2245465397269	40.0	39.0	41.0	36.0	41.0
26	39.15031880513582	40.0	39.0	41.0	36.0	41.0
27	39.067982647683934	40.0	39.0	41.0	36.0	41.0
28	39.00869071534632	40.0	39.0	41.0	35.0	41.0
29	38.88552129734766	40.0	39.0	41.0	35.0	41.0
30	38.81525315165808	40.0	38.0	41.0	35.0	41.0
31	38.771129938568144	40.0	38.0	41.0	35.0	41.0
32	38.66490523189798	40.0	38.0	41.0	35.0	41.0
33	38.54246367950621	40.0	38.0	41.0	35.0	41.0
34	38.53570908667424	40.0	38.0	41.0	35.0	41.0
35	38.50524063236964	40.0	38.0	41.0	35.0	41.0
36	38.440416921419626	40.0	38.0	41.0	34.0	41.0
37	38.408259818907034	40.0	38.0	41.0	34.0	41.0
38	38.34924447550004	40.0	38.0	41.0	34.0	41.0
39	38.184164555856405	40.0	38.0	41.0	34.0	41.0
40	38.169170524354385	40.0	38.0	41.0	34.0	41.0
41	38.17575042944071	40.0	38.0	41.0	34.0	41.0
42	38.06419774652808	40.0	37.0	41.0	34.0	41.0
43	37.15726264302559	39.0	36.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	2.0
20	5.0
21	5.0
22	23.0
23	38.0
24	58.0
25	79.0
26	135.0
27	158.0
28	207.0
29	345.0
30	448.0
31	588.0
32	793.0
33	1115.0
34	1749.0
35	2453.0
36	4257.0
37	7712.0
38	17575.0
39	30946.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.08090954086238	17.522636620374413	12.183014528197514	27.213439310565697
2	21.566657932279384	19.518444114478704	31.378286313215124	27.536611640026788
3	21.343931056569716	19.735348065333216	29.1946894925321	29.726031385564973
4	15.791772207179665	15.518094738987392	32.32596733339157	36.36416572044138
5	17.38579788627828	33.32896613969197	32.756863772673015	16.528372201356742
6	37.60008152094797	31.79608117157248	14.4364864471424	16.167350860337148
7	30.995429003988704	28.26447724692113	19.342300637610272	21.397793111479896
8	29.075319533001426	29.889073281509305	19.64072553643695	21.39488164905232
9	27.108626663172913	13.202026377849593	18.432468628992343	41.256878329985156
10	18.541648470026495	24.53052668355315	31.557341252511133	25.37048359390922
11	37.940722624974526	19.66692869828515	18.71051329082598	23.681835385914347
12	23.572655544880195	24.226278859871314	26.15512271814132	26.04594287710717
13	33.2226977610854	17.0742714065275	22.252307333973857	27.450723498413254
14	25.347191894488603	19.714967828340175	21.777738958278743	33.16010131889248
15	28.679360642850902	24.834774507234982	19.91877019827059	26.56709465164352
16	28.493027047485953	23.390689143156607	21.25076425888724	26.8655195504702
17	25.610679244184354	24.513057908987683	22.61187294377966	27.264389903048304
18	26.10126066323114	21.923312079657613	24.510146446560107	27.46528081055114
19	26.922293067807963	23.018021952426704	23.70949427897633	26.350190700789007
20	27.865606894343024	21.786473345561475	23.01074329635776	27.337176463737734
21	28.896264593705418	22.076163857105424	22.02521326462282	27.002358284566334
22	28.58764957638222	22.294523539173728	22.02521326462282	27.092613619821236
23	27.434710455061577	21.966984016071272	22.221736978484294	28.376568550382856
24	26.893178443532186	23.392144874370395	22.7996622703584	26.91501441173902
25	27.54097883366815	22.601682825283138	22.089265438029525	27.768072903019185
26	26.983433778787084	23.02384487728186	22.17078638600169	27.82193495792937
27	27.77244009666055	22.338195475587387	22.488135790607622	27.40122863714444
28	26.54234722100911	22.263953183684166	23.38923341194282	27.804466183363903
29	25.66017410545317	22.793839345503248	24.109820362768218	27.43616618627537
30	25.8625207441698	23.92057530497569	24.12001048126474	26.096893469589777
31	27.481293853902816	23.702215622907385	21.380324336914434	27.43616618627537
32	25.686377267301364	23.15631641773663	23.8419658194311	27.315340495530904
33	25.936763036073017	22.243572946691124	24.025387952368472	27.794276064867386
34	26.38076105627857	22.43281800448365	24.055958307858035	27.130462631379743
35	26.56709465164352	22.09072116924331	24.84642035694529	26.495763822167874
36	25.732960666142603	23.207267010219233	23.913296648906744	27.14647567473142
37	26.529245640085016	22.093632631670886	24.15494803039567	27.22217369784843
38	25.358837744198908	21.849069787754388	25.543715608350077	27.248376859696627
39	25.987713628555625	21.338108131714563	25.955687541852274	26.718490697877545
40	24.117099018837163	20.86208402480566	27.770984365446765	27.249832590910415
41	23.943867004396306	21.010568608612108	27.56718199551635	27.478382391475236
42	24.101085975485486	20.956706553701924	28.40713890587242	26.53506856494017
43	22.77054764608263	20.52872157684805	28.871517163071008	27.82921361399831
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	3.5
4	6.0
5	6.0
6	7.0
7	8.0
8	7.0
9	6.0
10	11.5
11	17.0
12	17.0
13	27.5
14	38.0
15	63.0
16	88.0
17	79.5
18	71.0
19	71.0
20	101.5
21	132.0
22	127.5
23	123.0
24	157.5
25	192.0
26	192.0
27	251.5
28	311.0
29	428.0
30	545.0
31	698.5
32	852.0
33	852.0
34	1047.0
35	1242.0
36	1451.0
37	1660.0
38	1985.0
39	2310.0
40	2310.0
41	2583.0
42	2856.0
43	3073.0
44	3290.0
45	3661.0
46	4032.0
47	4032.0
48	4429.0
49	4826.0
50	5244.5
51	5663.0
52	6295.0
53	6927.0
54	6927.0
55	6627.5
56	6328.0
57	6425.0
58	6522.0
59	6066.5
60	5611.0
61	5611.0
62	5142.0
63	4673.0
64	4123.5
65	3574.0
66	3024.5
67	2475.0
68	2475.0
69	2068.0
70	1661.0
71	1401.0
72	1141.0
73	857.0
74	573.0
75	573.0
76	441.5
77	310.0
78	282.5
79	255.0
80	219.5
81	184.0
82	184.0
83	136.0
84	88.0
85	64.5
86	41.0
87	38.5
88	36.0
89	36.0
90	27.0
91	18.0
92	12.0
93	6.0
94	3.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	68694.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.03010452150116
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.55624790119772	72.2217369784843
2	3.6920264169247416	5.7617841441756195
3	1.1771948807880304	2.7556991877019827
4	0.654826312451028	2.043846624159315
5	0.42722286481847693	1.6668122397880454
6	0.25931868213872616	1.2140798322997641
7	0.20521622327525094	1.1209130346172884
8	0.16976978470952575	1.0597723236381635
9	0.11380172381627553	0.7991964363699887
>10	0.7126599753740531	9.194398346289342
>50	0.02611842841685012	1.4018691588785046
>100	0.005596806089325025	0.7598916935976942
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT	270	0.39304742772294526	RNA PCR Primer, Index 26 (100% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT	131	0.19070078900631787	No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC	121	0.17614347686843101	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	97	0.14120592773750254	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	84	0.12228142195824963	No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC	74	0.10772410982036278	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	72	0.1048126473927854	No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC	71	0.10335691617899671	RNA PCR Primer, Index 26 (100% over 23bp)
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	69	0.10044545375141935	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.001455731213788686	0.0
27	0.0	0.0	0.0	0.014557312137886861	0.0
28	0.0	0.0	0.0	0.07569802311701168	0.0
29	0.0	0.0	0.0	0.14120592773750254	0.0
30	0.0	0.0	0.0	0.24747430634407663	0.0
31	0.0	0.0	0.0	0.5808367543016857	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGTCT	40	1.5712503E-6	32.375	36
TGCGCGC	25	0.005460863	29.599998	19
TTGCGCG	25	0.005460863	29.599998	18
ATTTGCG	25	0.005460863	29.599998	16
ATGCCGT	45	3.9365696E-6	28.777777	34
TATGCCG	50	8.929628E-6	25.899998	33
CCGTCTT	50	8.929628E-6	25.899998	37
TGCCGTC	50	8.929628E-6	25.899998	35
GGTATCA	80	2.6324415E-8	23.125	1
GGAGTGC	50	2.666415E-4	22.199999	23
CTTATAC	45	0.0037898282	20.555555	37
TTTGCGC	45	0.0037898282	20.555555	17
TGGAGTG	55	5.075718E-4	20.181818	22
ACATCTA	55	5.075718E-4	20.181818	24
CGGACAT	60	9.1167947E-4	18.5	21
GAGTGCA	60	9.1167947E-4	18.5	24
CGTATGC	70	1.1981474E-4	18.5	31
ATCTAAG	60	9.1167947E-4	18.5	26
TCTTATA	80	1.5809064E-5	18.5	37
CATCTAA	60	9.1167947E-4	18.5	25
>>END_MODULE